scholarly journals Follicular phase cycle programming using estradiol in oocyte donors – A convenient and effective approach

F&S Reports ◽  
2022 ◽  
Author(s):  
Manish Banker ◽  
Parul Arora ◽  
Jwal Banker ◽  
Reena Gupta ◽  
Sandeep Shah
2007 ◽  
Vol 292 (3) ◽  
pp. E900-E906 ◽  
Author(s):  
Christopher R. McCartney ◽  
Susan K. Blank ◽  
John C. Marshall

Progesterone (P) is the primary effector of LH (and by inference gonadotropin-releasing hormone) pulse frequency slowing in cycling women, but the time course of this action is unclear. We hypothesized that P administration to estradiol (E2)-pretreated women would slow LH pulse frequency within 12 h. We studied eight normally cycling women in two separate cycles (follicular phase, cycle days 7–11). After 3 days of E2 pretreatment (0.2 mg/day via transdermal patches), a 25-h blood sampling protocol (starting at 0800) was performed to define LH pulsatility. Oral micronized P (100 mg) or placebo (PBO) was administered at 1800 in a randomized, double-blind fashion, with treatment crossover occurring during a subsequent cycle. The 10-h mean P concentration increased from 0.6 ± 0.1 ng/ml before P (0800–1800) to 3.9 ± 0.3 ng/ml after P administration (2200–0800, P < 0.01). Ten-hour mean LH interpulse interval increased significantly after both P and PBO administration, with no significant difference between P and PBO. In contrast, mean LH, LH amplitude, and mean FSH increased significantly within 4 h of P administration, but not after PBO. We conclude that, in E2-pretreated women in the late follicular phase, 1) nocturnal LH pulse frequency is not acutely (within 12 h) influenced by P administration; 2) an acute increase in P causes pronounced augmentation of gonadotropin pulse amplitude within 4 h; and 3) LH pulse frequency slows overnight during the second half of the follicular phase.


2008 ◽  
Vol 53 (No. 2) ◽  
pp. 70-76 ◽  
Author(s):  
M. Machatkova ◽  
P. Hulinska ◽  
J. Horakova ◽  
Z. Reckova ◽  
K. Hanzalova

The objective of the study was to characterize the effect of the oestrous cycle stage on the yield, morphology and meiotic competence of porcine oocytes. A total of 46 cycling gilts, at 8.5–9 months of age, were used as oocyte donors. Their oestrous cycle was synchronized by Regumate and the onset of oestrus was checked (Day 0). The gilts were slaughtered at the early (Days 1–5), middle (6–10) and late (11–14) luteal or early (Days 15–16), middle (17–19) and late (20–21) follicular phase. Oocytes were isolated separately from medium (5–9 mm) and small (≤ 4 mm) follicles. Cumulus-oocyte complexes with dark, evenly granulated cytoplasm and at least two compact layers of cumulus cells were selected as useful for maturation. They were matured by a standard protocol, denuded from cumulus cells, fixed in glutaraldehyde, stained with 33258-Hoechst and examined by epifluorescence. The oocytes collected from small and medium follicles differed in their yield, morphology and meiotic competence regardless of the phase. The mean number (± S.E.M.) of oocytes isolated per donor was higher (187.7 ± 48.4 vs. 16.9 ± 6.1) but the mean percentage (± S.E.M.) of useful oocytes was lower (22.4 ± 7.5% vs. 80.2 ± 6.8%; <I>P</I> < 0.01) for small than for medium follicles. The mean number (± S.E.M.) of useful oocytes per donor was significantly (<I>P</I> < 0.01) higher (42.1 ± 16.8 vs. 11.9 ± 4.3) but the mean percentage of matured oocytes was significantly (<I>P</I> < 0.01) lower (48.4 ± 17.8% vs. 79.9 ± 7.9%) for small than for medium follicles. The oocyte population collected from small follicles varied during the oestrous cycle. The mean number (± S.E.M.) of oocytes isolated per donor from small follicles increased during the luteal and decreased during the follicular phase, except for the late follicular phase when it increased again. The mean percentage (± S.E.M.) of useful oocytes did not differ too much during this period, except for the late follicular phase when it decreased significantly (<I>P</I> < 0.01). The mean number (± S.E.M.) of useful oocytes per donor increased during the luteal and decreased during the follicular phase, but the differences were not significant except for the late follicular phase (<I>P</I> < 0.01). Similarly, the mean percentage (± S.E.M.) of matured oocytes increased during the luteal and decreased during the follicular phase, and the differences were significant. Compared with the oocyte population from small follicles, the oocyte population from medium follicles was less variable in the period from the middle luteal to middle follicular phase, when these follicles were present on the ovaries. It can be concluded that the porcine oocyte population changes in terms of quantity, morphological quality and meiotic competence according to the stage of follicular development. The late luteal and early follicular phases appeared to be most productive for oocyte recovery, because more morphologically normal oocytes with greater meiotic competence were collected, as compared with the other stages of the oestrous cycle.


1998 ◽  
Vol 5 (1) ◽  
pp. 93A-93A
Author(s):  
E CHEN ◽  
M LUTHER ◽  
A MORENO ◽  
T KING ◽  
R SCHENKEN

Author(s):  
Shehnaz Shaikh

Introduction: Menstrual cycle or menstruation involved discharge of sanguinous fluid and a sloughing of uterine wall. In women menstruation occurs at regular intervals on an average of 28 days, although most women gave a history of regular intervals of 28 to 30 days. About 10% -15% of women showed cycle at the precise 28 ± 2 days intervals when menstrual calendar was utilized. Normally in young women in different phases of ovarian cycles the plasma levels of estrogen vary. Ovulation occurs in the first 12-13th day of menstrual cycle, which is termed estrogen surge and second occurs in mid-luteal phase. During mid cycle or follicular phase of menstrual cycle the plasma concentration of progesterone is very low about 0.9 ng/mL. its level starts rising owing to secretion from the granulose cells. During luteal phase progesterone level reaches its peak value of 18 ng/mL and its level fall to a minimum value toward the end of the cycle. Estrogen affects local and systemic vasodilation. The menstrual cycle envelops two fundamental stages, the follicular stage (FP) and the luteal stage (LP). The follicular stage can part advance into two substages; the early FP, which is characterised with moo concentrations of both the key hormones estrogen and progesterone; and the mid FP where estrogen is tall autonomously from progesterone. The LP is epitomized by tall concentration of both estrogen and progesterone. These two fundamental stages are isolated by a soak surge in luteinizing hormone activating ovulation. These recurrent changes are said to be frequency unsurprising while long time. Aim: The main aim of this study is to evaluate the Cardiorespiratory functions changes during different Phases of Menstrual Cycle.   Material and methods: In this study, 20 with normal weight, 20 with obese and 20 with overage were included and taken them as a sample size. In this study all the young women those were recruited as a sample size are unmarried, undergraduate female student with the between the age group of 18-22years, having regular 28+6 days menstrual cycle for at least last 6months prior to this study. For the collection of data all the participants were instructed to attend the physiology lab department during each of three different phases. Day-2 during menstrual phase, Day-7, during follicular phase and Day-22 during luteal phase and the following parameters were recorded as Anthropometric measurements, measuring of pulse rate and blood pressure and cardiac efficiency test. Result: In general, work out proficiency changed essentially amid the distinctive stages of the menstrual cycle with the most elevated amid luteal stage and least amid menstrualo stage. There was no critical contrast in impact test amid menstrual stage, follicular stage and luteal stage of menstrual cycle among three bunches of people. Conclusion: We have watched noteworthy increment in cardiac and respiratory proficiency within the luteal stage of the menstrual cycle in ordinary weight people. Lower wellness levels were watched in overweight and stout females. In this manner hone of customary work out and admissions of solid slim down which offer assistance in lessening the weight and in turn the BMI will offer assistance in improving the physical wellness of the people. Keywords: Cardiorespiratory, Menstrual cycle, expiratory blast test


1965 ◽  
Vol 49 (2) ◽  
pp. 248-261 ◽  
Author(s):  
S. Mancuso ◽  
Francesca P. Mancuso ◽  
K.-G. Tillinger ◽  
E. Diczfalusy

ABSTRACT Two amenorrhoeic women were given a course of 10 injections of human menopausal gonadotrophin (HMG) in daily doses corresponding to 260 IU of follicle stimulating hormone (FSH) activity and 165 IU of interstitial cell stimulating hormone (ICSH) activity. In both patients an extensive ovarian stimulation was observed as indicated by the greatly increased urinary excretion of oestrone, 17β-oestradiol and oestriol. When HMG-treatment was followed subsequently by the administration of human chorionic gonadotrophin (HCG) for 5 days in a total dose of 18 000 and 30 000 IU, respectively, functional corpus luteum tissue was formed in both patients as evidenced by a huge rise in urinary pregnane-3α,20α-diol excretion and by the secretory transformation of a previously atrophic endometrium. At the approximate height of the follicular phase tracer doses of 3H-labelled dehydroepiandrosterone sulphate (DHAS) and 14C-labelled dehydroepiandrosterone (DHA) were administered to both patients in the form of a continuous intravenous infusion of 10 hours' duration. Infusion of the same dose was repeated under identical experimental conditions at the approximate height of the luteal phase. In both patients, very little radioactive material was associated with oestrone and 17β-oestradiol and none with oestriol isolated from 96-hours' urine specimens obtained at both phases of ovarian stimulation. It is concluded that — in contradistinction to the situation in pregnant women — circulating DHAS is not a significant precursor of urinary oestrogens in non-pregnant women.


1979 ◽  
Vol 90 (2) ◽  
pp. 372-384 ◽  
Author(s):  
N. P. Goncharov ◽  
A. G. Taranov ◽  
A. V. Antonichev ◽  
V. M. Gorlushkin ◽  
T. Aso ◽  
...  

ABSTRACT Adult baboons (5 males and 5 females) were exposed to immobilization stress by being strapped to a table in a horizontal position for 2 h. In females the experiment was performed during both the follicular and luteal phase. Peripheral blood was withdrawn at frequent intervals, the first sample just before immobilization, and the last one 3 days later. A number of steroids were measured in blood plasma samples by radioimmunoassay (17-hydroxypregnenolone, 17-hydroxyprogesterone, pregnenolone, testosterone, dihydrotestosterone, progesterone, 20α-dihydroprogesterone, oestrone, oestradiol) or competitive protein binding (cortisol) techniques. The cortisol levels exhibited a marked increase in both sexes. This increase was observed already during the immobilization and lasted for approximately 24 h. A similar, even more pronounced increase was seen in 17-hydroxypregnenolone, 17-hydroxyprogesterone and pregnenolone levels. A marked, long-lasting (72 h) decrease of testosterone and dihydrotestosterone levels was a consistent finding in male baboons. This was not observed in the females which, on the other hand, exhibited a marked decrease (duration 48 h) of progesterone and 20α-dihydroprogesterone levels during the luteal phase, and a significant decrease (duration > 24 h) of oestradiol and oestrone concentrations during the follicular phase. It is concluded that stress has a marked inhibitory action on gonadal function both in male and female baboons. In females the inhibition of steroidogenetic function is exerted both on the ovarian follicles and on the corpus luteum.


2009 ◽  
Vol E92-B (9) ◽  
pp. 2823-2837 ◽  
Author(s):  
Hye Kyung LEE ◽  
Won-Jin YOON ◽  
Tae-Jin LEE ◽  
Hyunseung CHOO ◽  
Min Young CHUNG

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