Ovarian stimulation in oocyte donors is associated with high expression of mRNA coding for apoptotic markers in cumulus cells

2016 ◽  
Author(s):  
Rocio Nunez - Calonge
Keyword(s):  
Ovarian Stimulation ◽  
Cumulus Cells ◽  
High Expression ◽  
Apoptotic Markers ◽  
Oocyte Donors

scholarly journals Comparative protein expression profiling in human cumulus cells in relation to oocyte fertilization and ovarian stimulation protocol

2006 ◽  
Vol 13 (6) ◽  
pp. 807-814 ◽  
Author(s):  
Samir Hamamah ◽  
Valérie Matha ◽  
Cyril Berthenet ◽  
Tal Anahory ◽  
Vanessa Loup ◽  
...  
Keyword(s):  
Protein Expression ◽  
Ovarian Stimulation ◽  
Expression Profiling ◽  
Cumulus Cells ◽  
Stimulation Protocol ◽  
Oocyte Fertilization

scholarly journals Acceptability and results of corifollitropin alfa (FSH-CFT) and desogestrel (DSG) for ovarian stimulation (COH) in oocyte donors (OD)

2017 ◽  
Vol 108 (3) ◽  
pp. e232 ◽  
Author(s):  
F. Martinez ◽  
D. Rodriguez-Barredo ◽  
J. Rodriguez-Purata ◽  
E. Clua ◽  
I. Rodriguez ◽  
...  
Keyword(s):  
Ovarian Stimulation ◽  
Corifollitropin Alfa ◽  
Oocyte Donors

scholarly journals Zinc concentrations in serum and follicular fluid during ovarian stimulation and expression of Zn2+ transporters in human oocytes and cumulus cells

2011 ◽  
Vol 22 (6) ◽  
pp. 647-652 ◽  
Author(s):  
Yves Ménézo ◽  
Laurène Pluntz ◽  
Jacques Chouteau ◽  
Timur Gurgan ◽  
Aygul Demirol ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Ovarian Stimulation ◽  
Cumulus Cells ◽  
Human Oocytes ◽  
Zinc Concentrations

P-631 Embryo euploidy rates following follicular or luteal start ovarian stimulation. A prospective study with repeated ovarian stimulation ovarian stimulation cycles

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
F Martinez ◽  
E Clua ◽  
M Roca ◽  
S Garcia ◽  
M Parriego ◽  
...  
Keyword(s):  
Prospective Study ◽  
Ovarian Stimulation ◽  
Luteal Phase ◽  
Gnrh Antagonist ◽  
Infertile Women ◽  
Oocyte Donors ◽  
A Prospective Study ◽  
Antagonist Protocol ◽  
Euploidy Rates ◽  
Euploidy Rate

Abstract Study question Is there any difference in embryo euploidy rates following luteal phase phase (LS) and follicular phase (FS) start ovarian stimulation. Summary answer The number of euploid blastocysts and embryo euploidy rate are comparable when comparing FS and LS. What is known already Random start ovarian stimulation (starting at any time of the cycle) has been traditionally used in women undergoing urgent fertility preservation for medical reason. Although there is accumulating evidence that in infertile women, LS can result in equivalent number of oocytes and embryos as compared with FS, no study has evaluated the effect of luteal phase start ovarian stimulation on embryo euploidy rates. The current study is the first prospective study designed to evaluate embryo euploidy rates in donors undergoing two identical consecutive ovarian stimulation protocols within a period of 6 months starting either in the (FS), or (LS). Study design, size, duration In a prospective study, conducted between May 2018 and January 2020, 40 oocyte donors underwent two consecutive ovarian stimulation protocols within a period of 6 months with an identical fixed GnRH antagonist protocol starting either in the early follicular (FS), or and luteal menstrual cycle phase (LS). Participants/materials, setting, methods All participants underwent two identical consecutive ovarian stimulation cycles with 150μg corifollitropin alfa followed by 200 IU rFSH in a fixed GnRH antagonist protocol either in the FS or LS. Six MII oocytes from the same oocyte donor, from each stimulation cycle, were allocated to the recipients and were inseminated with the same sperm sample (recipients partner sperm or donor sperm). Embryos were cultivated to blastocyst stage followed by preimplantation genetic testing for aneuploidies (PGT-A). Main results and the role of chance When comparing FP with LP, the duration of ovarian stimulation was significantly shorter (9.68± 2.09 vs 10.93± 1.55 days), 95% CI [-1.95; -0.55] and a higher total additional dose of daily recFSH was significantly lower (526.14± 338.94 IU vs 726.14± 366.27), 95% CI [-315,12; -84,88] when CPT was administered in the luteal phase. . There were no differences in the hormone values on the triggering day (Estradiol 2137.61±1198.25 pg/ml vs 2362.96±1472.89); 95% CI [-1160.45;709.76]. Overall no differences were observed in the number of oocytes (24.84± 11.200 vs 24.27± 9.08); 95% CI[-2,61; 3.75] and MII oocytes (21.41±10.19 vs 21.59± 8.81), 95%CI [-2.72; 2.35] retrieved between FP and LP cycles in the oocytes donors. Following oocyte allocation and fertilization to the recipients, a total of 245 blastocysts were biopsied (blastocyst formation rate 245/408, 60.05%), 117 in FP group and 128 in LP group. The overall blastocyst euploidy rate was 59.18% . There were no differences in the number of euploid embryos between FS (1.59±1.32) and LS (1.70±1.29), mean difference 0.11, 95%CI [-0.65; 0.46]. Finally, there were no differences in the percentage of euploid embryos per oocytes inseminated between FS [70/287 (24.4%)] and LP [75/278 (24.7%), mean difference -0.027, 95%CI [-0.11; 0.06]. Limitations, reasons for caution The study was performed in oocyte derived from potentially fertile young oocyte donors thus caution is needed when extrapolating the results in oocytes derived from infertile women of older age. Wider implications of the findings Luteal phase stimulation does not alter embryo euploidy status as compared with follicular phase stimulation and thus it appears that it can be safely used not only in cases of urgent medical fertility preservation but also in patients undergoing ovarian stimulation for IVF/ICSI. Trial registration number Clinical Trials Gov (NCT03555942).

scholarly journals OC06.01: Follicular tracking with three-dimensional ultrasound increases ovarian stimulation efficiency in oocyte donors

2012 ◽  
Vol 40 (S1) ◽  
pp. 11-11
Author(s):  
A. Rodriguez ◽  
J. Guillen ◽  
A. Blazquez ◽  
O. Coll ◽  
R. Vassena ◽  
...  
Keyword(s):  
Ovarian Stimulation ◽  
Three Dimensional ◽  
Oocyte Donors ◽  
Stimulation Efficiency

scholarly journals Impact of vitamin D levels on ovarian reserve and ovarian response to ovarian stimulation in oocyte donors

2017 ◽  
Vol 35 (2) ◽  
pp. 139-144 ◽  
Author(s):  
Alberta Maria Fabris ◽  
Maria Cruz ◽  
Carlos Iglesias ◽  
Alberto Pacheco ◽  
Azadeh Patel ◽  
...  
Keyword(s):  
Vitamin D ◽  
Ovarian Reserve ◽  
Ovarian Stimulation ◽  
Ovarian Response ◽  
Vitamin D Levels ◽  
Oocyte Donors

scholarly journals Pilot study: Alternative spliced variants of the Follicle-Stimulating Hormone Receptor gene in Greek women undergoing In Vitro Fertilisation (IVF)

2019 ◽  
Vol 18 (4) ◽  
pp. 155-162
Author(s):  
Eudoxia Mamas ◽  
Despoina Mavrogianni ◽  
Rami Raouasnte ◽  
Spyros Karkatzoulis ◽  
Emmanouela Liokari ◽  
...  
Keyword(s):  
Ovarian Stimulation ◽  
Hormone Receptor ◽  
Splice Variants ◽  
Receptor Gene ◽  
Ovarian Response ◽  
Cumulus Cells ◽  
Response To Treatment ◽  
In Vitro Fertilisation ◽  
Nucleotide Polymorphisms

Assisted Reproduction Technology (ART) has proven to be a valuable tool for infertile couples. Unfortunately, no perfect ovarian stimulation protocol has been designed and ovarian response shows great variability among women. Pharmacogenomics aims at detecting genetic markers so as to individualize protocols in order to maximize ovarian response to treatment. Follicle Stimulation hormone receptor (FSHR) mutations and single nucleotide polymorphisms have been extensively studied. Splice variants of the FSHR have been detected in women undergoing in vitro fertilisation (IVF). This study aims to determine the presence FSH splice variants in Greek women undergoing IVF. RNA was extracted from cumulus cells from 35 women and analysed by real time PCR. Splice variants were detected by gel electrophoresis. Three cases of deletion of exon 9 and 2 cases of insertion of intron 8 were detected in our study group. No association between the presence of splice variants and response to ovarian stimulation was detected. Two live births were detected one in each variant group. Even though these two types of splice variant detected do not show any clinical correlation it is believed that variants of the FSHR may be associated with poor or high response to exogenous gonadotrophin so further research is necessary.

scholarly journals DNA methylation-based age prediction and telomere length in white blood cells and cumulus cells of infertile women with normal or poor response to ovarian stimulation

Aging ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 3761-3773 ◽  
Author(s):  
Scott J. Morin ◽  
Xin Tao ◽  
Diego Marin ◽  
Yiping Zhan ◽  
Jessica Landis ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Telomere Length ◽  
Ovarian Stimulation ◽  
Blood Cells ◽  
White Blood Cells ◽  
Poor Response ◽  
Cumulus Cells ◽  
Infertile Women ◽  
Age Prediction
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