Visualizing the effect of hypoxia on fluorescence kinetics in living HeLa cells using the fluorescent ubiquitination-based cell cycle indicator (Fucci)

2012 ◽  
Vol 318 (3) ◽  
pp. 288-297 ◽  
Author(s):  
Atsushi Kaida ◽  
Masahiko Miura
Keyword(s):  
Cell Cycle ◽  
Hela Cells ◽  
Fluorescence Kinetics ◽  
Cycle Indicator

scholarly journals Visualization of cell-cycle modification by ionizing irradiation in single HeLa cells using fluorescent ubiquitination-based cell-cycle indicator

2014 ◽  
Vol 55 (suppl 1) ◽  
pp. i127-i128
Author(s):  
K. Kaminaga ◽  
Y. Sakamoto ◽  
Y. Kanari ◽  
M. Noguchi ◽  
A. Yokoya
Keyword(s):  
Cell Cycle ◽  
Hela Cells ◽  
Ionizing Irradiation ◽  
Cycle Indicator

scholarly journals Screening of cytotoxic or cytostatic flavonoids with quantitative Fluorescent Ubiquitination-based Cell Cycle Indicator-based cell cycle assay

2018 ◽  
Vol 5 (12) ◽  
pp. 181303 ◽  
Author(s):  
Young-Hyun Go ◽  
Hyo-Ju Lee ◽  
Hyeon-Joon Kong ◽  
Ho-Chang Jeong ◽  
Dong Young Lee ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Real Time ◽  
Hela Cells ◽  
Live Cells ◽  
Specific Cell ◽  
Study Gene Expression ◽  
Single Clone ◽  
Cytostatic Compounds ◽  
Cycle Monitoring ◽  
Cycle Indicator

The Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) system can be used not only to study gene expression at a specific cell cycle stage, but also to monitor cell cycle transitions in real time. In this study, we used a single clone of FUCCI-expressing HeLa cells (FUCCI-HeLa cells) and monitored the cell cycle in individual live cells over time by determining the ratios between red fluorescence (RF) of RFP-Cdt1 and green fluorescence (GF) of GFP-Geminin. Cytotoxic and cytostatic compounds, the latter of which induced G2 or mitotic arrest, were identified based on periodic cycling of the RF/GF and GF/RF ratios in FUCCI-HeLa cells treated with anti-cancer drugs. With this cell cycle monitoring system, ten flavonoids were screened. Of these, apigenin and luteolin, which have a flavone backbone, were cytotoxic, whereas kaempferol, which has a flavonol backbone, was cytostatic and induced G2 arrest. In summary, we developed a system to quantitatively monitor the cell cycle in real time. This system can be used to identify novel compounds that modulate the cell cycle and to investigate structure–activity relationships.

Radiosensitivity of Early and Late M-Phase HeLa Cells Isolated by a Combination of Fluorescent Ubiquitination-Based Cell Cycle Indicator (Fucci) and Mitotic Shake-Off

2011 ◽  
Vol 176 (3) ◽  
pp. 407-411 ◽  
Author(s):  
Motoshi Nakayama ◽  
Atsushi Kaida ◽  
Shifumi Deguchi ◽  
Kengo Sakaguchi ◽  
Masahiko Miura
Keyword(s):  
Cell Cycle ◽  
Hela Cells ◽  
M Phase ◽  
Cycle Indicator

scholarly journals Apoptotic effect of novel pyrazolone-based derivative [Cu(PMPP-SAL)(EtOH)] on HeLa cells and its mechanism

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Delizhaer Reheman ◽  
Jing Zhao ◽  
Shan Guan ◽  
Guan-Cheng Xu ◽  
Yi-Jie Li ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Hela Cells ◽  
Copper Complexes ◽  
Antibacterial Properties ◽  
Inhibitory Effect ◽  
Parp Cleavage ◽  
Potential Candidate ◽  
Tnf Α

Abstract Pyrazolone complexes have strong anti-tumor and antibacterial properties, but the anti-tumor mechanism of pyrazolone-based copper complexes has not been fully understood. In this study, the possible mechanism and the inhibitory effect of a novel pyrazolone-based derivative compound [Cu(PMPP-SAL)(EtOH)] on human cervical cancer cells (HeLa cells) was investigated. [Cu(PMPP-SAL)(EtOH)] effectively inhibited proliferation of HeLa cells in vitro with an IC50 value of 2.082 after treatment for 72 h. Cell cycle analysis showed apoptosis was induced by blocking the cell cycle in the S phase. [Cu(PMPP-SAL)(EtOH)] promoted the loss of mitochondrial membrane potential, release of cytochrome c, PARP cleavage, and activation of caspase-3/9 in HeLa cells. Additionally, [Cu(PMPP-SAL)(EtOH)] inhibited the PI3K/AKT pathway and activated the P38/MAPK, and JNK/MAPK pathways. [Cu(PMPP-SAL)(EtOH)] also inhibited the phosphorylation of Iκ-Bα in the NF-κB pathway activated by TNF-α, thus restricting the proliferation of HeLa cells which were activated by TNF-α. In conclusion, [Cu(PMPP-SAL)(EtOH)] inhibited the growth of HeLa cells and induced apoptosis possibly via the caspase-dependent mitochondria-mediated pathway. These results suggest that [Cu(PMPP-SAL)(EtOH)] can be a potential candidate for the treatment of cervical cancer.

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