Metabolic compartmentalization in yeast mitochondria: Burden and solution for squalene overproduction

2021 ◽  
Author(s):  
Zhan-Tao Zhu ◽  
Meng-Meng Du ◽  
Bei Gao ◽  
Xin-Yi Tao ◽  
Ming Zhao ◽  
...  
Keyword(s):  
Yeast Mitochondria

scholarly journals Functional expression of a yeast mitochondrial intron-encoded protein requires RNA processing at a conserved dodecamer sequence at the 3' end of the gene.

1989 ◽  
Vol 9 (4) ◽  
pp. 1507-1512 ◽  
Author(s):  
H Zhu ◽  
H Conrad-Webb ◽  
X S Liao ◽  
P S Perlman ◽  
R A Butow
Keyword(s):  
Genetic Analysis ◽  
Rna Processing ◽  
Fold Increase ◽  
Functional Expression ◽  
Rrna Gene ◽  
Yeast Mitochondria ◽  
Wild Type ◽  
Site Specific ◽  
Var1 Gene ◽  
A Site

All mRNAs of yeast mitochondria are processed at their 3' ends within a conserved dodecamer sequence, 5'-AAUAAUAUUCUU-3'. A dominant nuclear suppressor, SUV3-I, was previously isolated because it suppresses a dodecamer deletion at the 3' end of the var1 gene. We have tested the effects of SUV3-1 on a mutant containing two adjacent transversions within a dodecamer at the 3' end of fit1, a gene located within the 1,143-base-pair intron of the 21S rRNA gene, whose product is a site-specific endonuclease required in crosses for the quantitative transmission of that intron to 21S alleles that lack it. The fit1 dodecamer mutations blocked both intron transmission and dodecamer cleavage, neither of which was suppressed by SUV3-1 when present in heterozygous or homozygous configurations. Unexpectedly, we found that SUV3-1 completely blocked cleavage of the wild-type fit1 dodecamer and, in SUV3-1 homozygous crosses, intron conversion. In addition, SUV3-1 resulted in at least a 40-fold increase in the amount of excised intron accumulated. Genetic analysis showed that these phenotypes resulted from the same mutation. We conclude that cleavage of a wild-type dodecamer sequence at the 3' end of the fit1 gene is essential for fit1 expression.

scholarly journals A maturase-like subunit of the sequence-specific endonuclease endo.SceI from yeast mitochondria

1991 ◽  
Vol 266 (3) ◽  
pp. 1977-1984
Author(s):  
K Nakagawa ◽  
N Morishima ◽  
T Shibata
Keyword(s):  
Yeast Mitochondria

Discriminate Raman/GFP Spectra of Yeast Mitochondria Using Convolutional Neural Network

2020 ◽  
Author(s):  
Nungnit Wattanavichean ◽  
Jirasin Boonchai ◽  
Sasithon Yodthong ◽  
Chakkrit Preuksakarn ◽  
Scott C.-H. Huang ◽  
...  
Keyword(s):  
Neural Network ◽  
Convolutional Neural Network ◽  
Yeast Mitochondria

Mitochondrial upstream promoter sequences modulate in vivo the transcription of a gene in yeast mitochondria

Mitochondrion ◽  
2006 ◽  
Vol 6 (6) ◽  
pp. 289-298 ◽  
Author(s):  
A. Pfeuty ◽  
C. Dufresne ◽  
M. Gueride ◽  
G. Lecellier
Keyword(s):  
Yeast Mitochondria ◽  
Promoter Sequences ◽  
Upstream Promoter
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