scholarly journals Nucleoprotein complex intermediates in HIV-1 integration

Methods ◽  
2009 ◽  
Vol 47 (4) ◽  
pp. 237-242 ◽  
Author(s):  
Min Li ◽  
Robert Craigie
Keyword(s):  
Nucleoprotein Complex ◽  
Hiv 1

scholarly journals Human Immunodeficiency Virus Type 1 Vif Protein Is Packaged into the Nucleoprotein Complex through an Interaction with Viral Genomic RNA

2001 ◽  
Vol 75 (16) ◽  
pp. 7252-7265 ◽  
Author(s):  
Mohammad A. Khan ◽  
Claudia Aberham ◽  
Sandra Kao ◽  
Hirofumi Akari ◽  
Robert Gorelick ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Genomic Rna ◽  
Immunodeficiency Virus ◽  
Nucleoprotein Complex ◽  
Vif Protein ◽  
Viral Genomic Rna ◽  
Hiv 1

ABSTRACT The human immunodeficiency virus type 1 (HIV-1) Vif protein plays a critical role in the production of infectious virions. Previous studies have demonstrated the presence of small amounts of Vif in virus particles. However, Vif packaging was assumed to be nonspecific, and its functional significance has been questioned. We now report that packaging of Vif is dependent on the packaging of viral genomic RNA in both permissive and restrictive HIV-1 target cells. Mutations in the nucleocapsid zinc finger domains that abrogate packaging of viral genomic RNA abolished packaging of Vif. Additionally, an RNA packaging-defective virus exhibited significantly reduced packaging of Vif. Finally, deletion of a putative RNA-interacting domain in Vif abolished packaging of Vif into virions. Virion-associated Vif was resistant to detergent extraction and copurified with components of the viral nucleoprotein complex and functional reverse transcription complexes. Thus, Vif is specifically packaged into virions as a component of the viral nucleoprotein complex. Our data suggest that the specific association of Vif with the viral nucleoprotein complex might be functionally significant and could be a critical requirement for infectivity of viruses produced from restrictive host cells.

scholarly journals A supramolecular assembly mediates lentiviral DNA integration

Science ◽  
2017 ◽  
Vol 355 (6320) ◽  
pp. 93-95 ◽  
Author(s):  
Allison Ballandras-Colas ◽  
Daniel P. Maskell ◽  
Erik Serrao ◽  
Julia Locke ◽  
Paolo Swuec ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Supramolecular Assembly ◽  
Structural Data ◽  
Higher Order ◽  
Viral Dna ◽  
Dna Integration ◽  
Cryo Electron Microscopy ◽  
Nucleoprotein Complex ◽  
Retroviral Integrase ◽  
Hiv 1

Retroviral integrase (IN) functions within the intasome nucleoprotein complex to catalyze insertion of viral DNA into cellular chromatin. Using cryo–electron microscopy, we now visualize the functional maedi-visna lentivirus intasome at 4.9 angstrom resolution. The intasome comprises a homo-hexadecamer of IN with a tetramer-of-tetramers architecture featuring eight structurally distinct types of IN protomers supporting two catalytically competent subunits. The conserved intasomal core, previously observed in simpler retroviral systems, is formed between two IN tetramers, with a pair of C-terminal domains from flanking tetramers completing the synaptic interface. Our results explain how HIV-1 IN, which self-associates into higher-order multimers, can form a functional intasome, reconcile the bulk of early HIV-1 IN biochemical and structural data, and provide a lentiviral platform for design of HIV-1 IN inhibitors.

Ultrastructural observations of human monocytes infected with HIV-1

1991 ◽  
Vol 49 ◽  
pp. 108-109
Author(s):  
James K. Koehler ◽  
Steven G. Reed ◽  
Joao S. Silva
Keyword(s):  
Gradient Centrifugation ◽  
Virus Production ◽  
Human Monocyte ◽  
Red Cross ◽  
Human Monocytes ◽  
Blood Monocytes ◽  
Hiv Replication ◽  
Electron Microscopic ◽  
Ultrastructural Studies ◽  
Hiv 1

As part of a larger study involving the co-infection of human monocyte cultures with HIV and protozoan parasites, electron microscopic observations were made on the course of HIV replication and infection in these cells. Although several ultrastructural studies of the cytopathology associated with HIV infection have appeared, few studies have shown the details of virus production in “normal,” human monocytes/macrophages, one of the natural targets of the virus, and suspected of being a locus of quiescent virus during its long latent period. In this report, we detail some of the interactions of developing virons with the membranes and organelles of the monocyte host.Peripheral blood monocytes were prepared from buffy coats (Portland Red Cross) by Percoll gradient centrifugation, followed by adherence to cover slips. 90-95% pure monocytes were cultured in RPMI with 5% non-activated human AB serum for four days and infected with 100 TCID50/ml of HIV-1 for four hours, washed and incubated in fresh medium for 14 days.

Astrocytic adhesion molecules are increased in HIV-1-associated cognitive/motor complex

1997 ◽  
Vol 23 (3) ◽  
pp. 83-92 ◽  
Author(s):  
D. Seilhean ◽  
A. Dzia-Lepfoundzou ◽  
V. Sazdovitch ◽  
B. Cannella ◽  
C. S. Raine ◽  
...  
Keyword(s):  
Adhesion Molecules ◽  
Motor Complex ◽  
Hiv 1

Ability of caregivers to read delayed hypersensitivity skin tests in children exposed to and infected by HIV*1

2000 ◽  
Vol 14 (2) ◽  
pp. 50-55
Author(s):  
FORTHEPEDIATRICPULMONARYANDCA ◽  
H COHEN ◽  
X CHEN ◽  
S SUNKLE ◽  
L DAVIS ◽  
...  
Keyword(s):  
Delayed Hypersensitivity ◽  
Skin Tests ◽  
Hypersensitivity Skin ◽  
Hiv 1

Primary HIV-1 infection in users of pre-exposure prophylaxis

2020 ◽  
Author(s):  
Juan Ambrosioni ◽  
Elisa Petit ◽  
Geoffroy Liegeon ◽  
Montserrat Laguno ◽  
José M Miró
Keyword(s):  
Exposure Prophylaxis ◽  
Hiv 1

HIV-Diagnostik in der Schweiz und ärztlicher Abklärungsgang von HIV-Infizierten

2014 ◽  
Vol 71 (8) ◽  
pp. 451-460
Author(s):  
Jörg Schüpbach ◽  
Christoph Berger ◽  
Jürg Böni ◽  
Roberto F. Speck
Keyword(s):  
Hiv Test ◽  
Hiv 1

„HIV-positiv“ ist auch heute noch eine belastende Diagnose. Falsch positive wie falsch negative HIV-Test-Resultate herauszugeben, muss unbedingt vermieden werden. Das Bundesamt für Gesundheit (BAG) hat ein HIV-Testkonzept entworfen, das dieser Anforderung gerecht wird und darüber hinaus das Virus im betroffenen Patienten detailliert für eine optimale medizinische Betreuung charakterisiert. Das Testkonzept fordert hierzu die Beantwortung der folgenden vier Fragen: 1. Ist die getestete Person tatsächlich HIV-infiziert? 2. Handelt es sich um eine Infektion mit HIV-1 oder HIV-2, und im Falle von HIV-1, um welche Virusgruppe, M oder O? Sind Resistenzen gegenüber den antiretroviralen Medikamenten vorhanden? 3. Wie hoch ist die Viruslast? 4. Wie hoch ist der Anteil frischer HIV-Infektionen an den neu gemeldeten Fällen? In diesem Artikel werden wir das Testkonzept besprechen. Abschliessend werden wir in einer kurzen Übersicht darlegen, was der Arzt bei einer Erst- und Folgekonsultation bei einem HIV-infizierten Patienten abklären sollte.

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