Immunobiological activities of synthetic peptide segments of fimbrial protein from Porphyromonas gingivalis

1991 ◽  
Vol 180 (3) ◽  
pp. 1335-1341 ◽  
Author(s):  
Tomohiko Ogawa ◽  
Yutaka Kusumoto ◽  
Hiroshi Uchida ◽  
Shigeru Nagashima ◽  
Hideji Ogo ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Synthetic Peptide ◽  
Peptide Segments ◽  
Fimbrial Protein

scholarly journals Porphyromonas gingivalis fimbrial protein Mfa5 contains a von Willebrand factor domain and an intramolecular isopeptide

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Thomas V. Heidler ◽  
Karin Ernits ◽  
Agnieszka Ziolkowska ◽  
Rolf Claesson ◽  
Karina Persson
Keyword(s):  
Porphyromonas Gingivalis ◽  
Von Willebrand Factor ◽  
Host Cells ◽  
Negative Bacterium ◽  
Oral Biofilm ◽  
Gram Negative ◽  
Type V ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Fimbrial Protein

AbstractThe Gram-negative bacterium Porphyromonas gingivalis is a secondary colonizer of the oral biofilm and is involved in the onset and progression of periodontitis. Its fimbriae, of type-V, are important for attachment to other microorganisms in the biofilm and for adhesion to host cells. The fimbriae are assembled from five proteins encoded by the mfa1 operon, of which Mfa5 is one of the ancillary tip proteins. Here we report the X-ray structure of the N-terminal half of Mfa5, which reveals a von Willebrand factor domain and two IgG-like domains. One of the IgG-like domains is stabilized by an intramolecular isopeptide bond, which is the first such bond observed in a Gram-negative bacterium. These features make Mfa5 structurally more related to streptococcal adhesins than to the other P. gingivalis Mfa proteins. The structure reported here indicates that horizontal gene transfer has occurred among the bacteria within the oral biofilm.

Novel synthetic peptide derived from Porphyromonas gingivalis Lys-gingipain detects IgG-mediated host response in periodontitis

Anaerobe ◽  
2020 ◽  
Vol 61 ◽  
pp. 102140
Author(s):  
Ellen Karla Nobre dos Santos-Lima ◽  
Kizzes Araújo Paiva Andrade Cardoso ◽  
Patrícia Mares de Miranda ◽  
Paulo Cirino de Carvalho-Filho ◽  
Thaise Passos Rocha ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Synthetic Peptide ◽  
Host Response

Purification and characterization of a putative fimbrial protein/receptor of Porphyromonas gingivalis

1998 ◽  
Vol 43 (1) ◽  
pp. 99-104 ◽  
Author(s):  
S. G. Dashper ◽  
N. M. O'Brien-Simpson ◽  
P. S. Bhogal ◽  
A. D. Franzmann ◽  
E. C. Reynolds
Keyword(s):  
Porphyromonas Gingivalis ◽  
Purification And Characterization ◽  
Protein Receptor ◽  
Fimbrial Protein

scholarly journals Capsular Polysaccharide-Fimbrial Protein Conjugate Vaccine Protects against Porphyromonas gingivalis Infection in SCID Mice Reconstituted with Human Peripheral Blood Lymphocytes

1998 ◽  
Vol 66 (1) ◽  
pp. 391-393 ◽  
Author(s):  
Jeom-Il Choi ◽  
Robert E. Schifferle ◽  
Fuminobu Yoshimura ◽  
Byung-Woo Kim
Keyword(s):  
Conjugate Vaccine ◽  
Porphyromonas Gingivalis ◽  
Capsular Polysaccharide ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Scid Mice ◽  
Protein Conjugate ◽  
Periodontal Infection ◽  
Fimbrial Protein

ABSTRACT The effect of immunization with either a Porphyromonas gingivalis fimbrial protein, a capsular polysaccharide, or a capsular polysaccharide-fimbrial protein conjugate vaccine were compared in hu-PBL-SCID mice. A significantly higher human immunoglobulin G antibody response and the highest degree of in vivo protection against bacterial challenge was observed in the group immunized with the conjugate vaccine. It was concluded that capsular polysaccharide-fimbrial protein conjugate from P. gingivalis could potentially be developed as a vaccine against periodontal infection by P. gingivalis.

Sign in / Sign up

Export Citation Format

Share Document