The formation of fatty acyl thioesters during 14C-1-acetate incorporation into long chain fatty acids by isolated spinach chloroplasts

1971 ◽  
Vol 44 (6) ◽  
pp. 1544-1551 ◽  
Author(s):  
C. Gamini Kannangara ◽  
P.K. Stumpf
Keyword(s):  
Fatty Acids ◽  
Fatty Acyl ◽  
Acetate Incorporation ◽  
Long Chain Fatty Acids ◽  
Spinach Chloroplasts ◽  
Long Chain ◽  
Chain Fatty Acids

Candida albicans fatty acyl-CoA synthetase, CaFaa4p, is involved in the uptake of exogenous long-chain fatty acids and cell activity in the biofilm

2017 ◽  
Vol 64 (2) ◽  
pp. 429-441 ◽  
Author(s):  
Kengo Tejima ◽  
Masanori Ishiai ◽  
Somay O. Murayama ◽  
Shun Iwatani ◽  
Susumu Kajiwara
Keyword(s):  
Fatty Acids ◽  
Candida Albicans ◽  
Cell Activity ◽  
Fatty Acyl ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids

scholarly journals Transmembrane Movement of Exogenous Long-Chain Fatty Acids: Proteins, Enzymes, and Vectorial Esterification

2003 ◽  
Vol 67 (3) ◽  
pp. 454-472 ◽  
Author(s):  
Paul N. Black ◽  
Concetta C. DiRusso
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Molecular Genetics ◽  
Fatty Acyl ◽  
Model Systems ◽  
Fatty Acid Transport ◽  
Long Chain Fatty Acids ◽  
Acid Transport ◽  
Long Chain ◽  
Chain Fatty Acids

SUMMARY The processes that govern the regulated transport of long-chain fatty acids across the plasma membrane are quite distinct compared to counterparts involved in the transport of hydrophilic solutes such as sugars and amino acids. These differences stem from the unique physical and chemical properties of long-chain fatty acids. To date, several distinct classes of proteins have been shown to participate in the transport of exogenous long-chain fatty acids across the membrane. More recent work is consistent with the hypothesis that in addition to the role played by proteins in this process, there is a diffusional component which must also be considered. Central to the development of this hypothesis are the appropriate experimental systems, which can be manipulated using the tools of molecular genetics. Escherichia coli and Saccharomyces cerevisiae are ideally suited as model systems to study this process in that both (i) exhibit saturable long-chain fatty acid transport at low ligand concentrations, (ii) have specific membrane-bound and membrane-associated proteins that are components of the transport apparatus, and (iii) can be easily manipulated using the tools of molecular genetics. In both systems, central players in the process of fatty acid transport are fatty acid transport proteins (FadL or Fat1p) and fatty acyl coenzyme A (CoA) synthetase (FACS; fatty acid CoA ligase [AMP forming] [EC 6.2.1.3]). FACS appears to function in concert with FadL (bacteria) or Fat1p (yeast) in the conversion of the free fatty acid to CoA thioesters concomitant with transport, thereby rendering this process unidirectional. This process of trapping transported fatty acids represents one fundamental mechanism operational in the transport of exogenous fatty acids.

X-Linked adrenoleukodystrophy: Defective peroxisomal oxidation of very long chain fatty acids but not of very long chain fatty acyl-CoA esters

1987 ◽  
Vol 165 (2-3) ◽  
pp. 321-329 ◽  
Author(s):  
R.J.A. Wanders ◽  
C.W.T. van Roermund ◽  
M.J.A. van Wijland ◽  
A.A. Nijenhuis ◽  
A. Tromp ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acyl ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids

scholarly journals Peroxisomal β-oxidation of long-chain fatty acids possessing different extents of unsaturation

1987 ◽  
Vol 247 (3) ◽  
pp. 531-535 ◽  
Author(s):  
R Hovik ◽  
H Osmundsen
Keyword(s):  
Fatty Acids ◽  
Substrate Inhibition ◽  
Fatty Acyl ◽  
Long Chain Fatty Acids ◽  
Beta Oxidation ◽  
Long Chain ◽  
Myristoyl Coa ◽  
Chain Fatty Acids

Rates of peroxisomal beta-oxidation were measured as fatty acyl-CoA-dependent NAD+ reduction, by using solubilized peroxisomal fractions isolated from livers of rats treated with clofibrate. Medium- to long-chain saturated fatty acyl-CoA esters as well as long-chain polyunsaturated fatty acyl-CoA esters were used. Peroxisomal beta-oxidation shows optimal specificity towards long-chain polyunsaturated acyl-CoA esters. Eicosa-8,11,14-trienoyl-CoA, eicosa-11,14,17-trienoyl-CoA and docosa-7,10,13,16-tetraenoyl-CoA all gave Vmax. values of about 150% of that obtained with palmitoyl-CoA. The Km values obtained with these fatty acyl-CoA esters were 17 +/- 6, 13 +/- 4 and 22 +/- 3 microM respectively, which are in the same range as the value for palmitoyl-CoA (13.8 +/- 1 microM). Myristoyl-CoA gave the higher Vmax. (110% of the palmitoyl-CoA value) of the saturated fatty acyl-CoAs tested. Substrate inhibition was mostly observed with acyl-CoA esters giving Vmax. values higher than 50% of that given by palmitoyl-CoA.

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