scholarly journals Efficacy of Testicular Sperm Retrieval by Percutaneous Fine Needle Aspiration in Men with Obstructive Azoospermia

2000 ◽  
Vol 74 (3) ◽  
pp. S76-S77
Author(s):  
A. Tallarini ◽  
A. Borini ◽  
A. Maccolini ◽  
M.A. Bonu ◽  
R. Sciajno ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Obstructive Azoospermia ◽  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Fine Needle ◽  
Testicular Sperm Retrieval

scholarly journals Successful Targeted Testicular Sperm Extraction Using Microsurgical Technique (microTESE) Following Fine Needle Aspiration (FNA) Mapping in a Non-Obstructive Azoospermia (NOA) Patient: A Case Report

2020 ◽  
Author(s):  
Ponco Birowo ◽  
William Tendi ◽  
Nur Rasyid ◽  
Paul J Turek ◽  
Ivan Rizal Sini ◽  
...  
Keyword(s):  
Male Infertility ◽  
Success Rate ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Testicular Sperm Extraction ◽  
Obstructive Azoospermia ◽  
Current Guideline ◽  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Fine Needle

Background: Management for male infertility can be difficult for some cases. Surgical intervention has long been thought as the last resort to help married couples to conceive. The current guideline recommends testicular sperm extraction with microsurgery technique (microTESE) in severe cases of male infertility. However, the success rate still varies. Thus, a new strategy was needed to further increase the sperm retrieval success rate. Case Presentation: A 39-year-old male with a history of failed sperm extraction, non-obstructive azoospermia (NOA) and Y-chromosomal microdeletion came to the fertility center to undergo sperm retrieval. Fine needle aspiration (FNA) Mapping was performed prior to microTESE to increase the accuracy of sperm retrieval. After further examination with laser assisted immotile sperm selection (LAISS), five spermatozoa were found. Conclusion: The combination of FNA Mapping and microTESE increases the chance of a successful sperm extraction.

scholarly journals Testicular fine needle aspiration: the alternative method for sperm retrieval in non-obstructive azoospermia

1999 ◽  
Vol 14 (7) ◽  
pp. 1785-1790 ◽  
Author(s):  
Aby Lewin ◽  
Benjamin Reubinoff ◽  
Anat Porat-Katz ◽  
David Weiss ◽  
Vered Eisenberg ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Obstructive Azoospermia ◽  
Sperm Retrieval ◽  
Fine Needle ◽  
Alternative Method

Relevance of testicular histopathology on prediction of sperm retrieval rates in case of non-obstructive and obstructive azoospermia

2018 ◽  
Vol 85 (2) ◽  
pp. 60-67 ◽  
Author(s):  
Gianmartin Cito ◽  
Maria E Coccia ◽  
Sara Dabizzi ◽  
Simone Morselli ◽  
Pier A Della Camera ◽  
...  
Keyword(s):  
Follicle Stimulating Hormone ◽  
Needle Aspiration ◽  
Testicular Sperm Extraction ◽  
Total Testosterone ◽  
Obstructive Azoospermia ◽  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Maturation Arrest ◽  
Fine Needle ◽  
Normal Spermatogenesis

Introduction: The aim of our research was to establish the relevance of testicular histopathology on sperm retrieval after testicular sperm extraction in patients with non-obstructive azoospermia and in patients with obstructive azoospermia, who already underwent a previous failure testicular fine needle aspiration. Methods: We evaluated a total of 82 azoospermic men, underwent testicular sperm extraction, referring to the Assisted Reproductive Technology Centre of the University of Florence, Italy between January 2008 and March 2017. A general and genital physical examination, scrotal and trans-rectal ultrasound, semen analysis, hormone measurements, including follicle-stimulating hormone, luteinizing hormone and total testosterone, were collected. Results: Successful sperm retrieval was obtained in 36 men of total (43.9%). Successful sperm retrieval was 29.5% in non-obstructive azoospermia patients, while men with obstructive azoospermia, who, underwent a previous failure testicular fine needle aspiration, had sperm retrieval in 86% of cases. Mean luteinizing hormone was 6.55 IU/L, total testosterone 4.70 ng/mL, right testicular volume 13.7 mL and left testicular volume 13.6 mL. Mean Follicle-stimulating hormone was 13.45 IU/L in patients with negative sperm retrieval and 8.18 IU/L in men with successful sperm retrieval. According to histology, 20.7% had normal spermatogenesis, 35.3% hypospermatogenesis, 35.3% maturation arrest and 8.5% Sertoli cell-only syndrome. Successful sperm retrieval was 88.2% in patients with normal spermatogenesis, 24.1% in the maturation arrest group and 48.27% in patients with hypospermatogenesis, while negative sperm retrieval was reported in Sertoli cell-only syndrome patients. Seven cases with maturation arrest showed a successful sperm retrieval. Conclusion: Testicular histopathology after testicular sperm extraction offers important information on prediction of sperm retrieval and can guide the surgeon in choosing the more suitable therapeutic practice.

Successful pregnancy in double factor infertility: Sperm in vitro culture by modified testicular fine-needle aspiration

2019 ◽  
Vol 86 (3) ◽  
pp. 141-144
Author(s):  
Gianmartin Cito ◽  
Maria Elisabetta Coccia ◽  
Francesco Bertocci ◽  
Rita Picone ◽  
Andrea Cocci ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Sperm Motility ◽  
Fine Needle Aspiration ◽  
Intracytoplasmic Sperm Injection ◽  
Vas Deferens ◽  
Needle Aspiration ◽  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Fine Needle

Introduction: Infertility may depend up to 27% of couples on both partners. In patients with obstructive azoospermia, testicular fine-needle aspiration represents a good option to retrieve spermatozoa, in order to perform an assisted reproductive treatment. In vitro maturation of testicular spermatozoa could be the better choice of treatment in view of the increased motility, improving fertilization and pregnancy rates. Case description: A 34-year-old azoospermic man and his 33-year-old partner referred for treatment of simultaneous male and female infertility factor. The woman presented a diminished ovarian reserve, with serum follicle stimulating hormone value of 27.15 IU/L. The man underwent trans-rectal and testicular ultrasounds that detected the congenital absence of proximal vas deferens on the right side and the absence of seminal vesicle and distal vas deferens on the left side. We proposed a chance to have their own biological child. The man underwent modified testicular fine-needle aspiration using a 18-gauge butterfly needle. Sperm retrieval was successful with 0.001 × 106 spermatozoa/mL and absence of motility. Testicular sperm suspension was cultured for 24 h to identify sperm viability, achieving 10% of sperm motility. Two metaphase II oocytes were retrieved and processed with intracytoplasmic sperm injection. Clinical pregnancy with live birth was obtained. Conclusion: Performing modified testicular fine-needle aspiration increases successful sperm retrieval. Testicular sperm in vitro culture for 24 h proved to be a real and practical technique to increase sperm motility, in order to select mature and viable spermatozoa and improve successful intracytoplasmic sperm injection outcomes.

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