Blockade of NK1 receptors in the spinal cord reduces the hypersensitivity associated with colorectal distension in an animal model of the irritable bowel syndrome

2000 ◽  
Vol 118 (4) ◽  
pp. A841 ◽  
Author(s):  
Motohiro Kawasaki ◽  
Pankaj J. Pasricha ◽  
Elie D. Al-Chaer
Keyword(s):  
Spinal Cord ◽  
Animal Model ◽  
Irritable Bowel Syndrome ◽  
Colorectal Distension ◽  
Nk1 Receptors ◽  
Irritable Bowel

scholarly journals Mild Moxibustion Decreases the Expression of Prokineticin 2 and Prokineticin Receptor 2 in the Colon and Spinal Cord of Rats with Irritable Bowel Syndrome

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Cili Zhou ◽  
Jimeng Zhao ◽  
Luyi Wu ◽  
Renjia Huang ◽  
Yin Shi ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Irritable Bowel Syndrome ◽  
Rat Model ◽  
Rat Colon ◽  
Spinal Cord Tissue ◽  
Model Group ◽  
Colorectal Distension ◽  
Prokineticin 2 ◽  
Mild Moxibustion ◽  
Irritable Bowel

It has been proven that prokineticin 2 (PK2) and its receptor PKR2 play an important role in hyperalgesia, while mild moxibustion can relieve visceral hypersensitivity in a rat model of irritable bowel syndrome (IBS). The goal of the present study was to determine the effects of mild moxibustion on the expression of PK2 and PKR2 in colon and spinal cord in IBS rat model, which was induced by colorectal distension using inflatable balloons. After mild moxibustion treatment, abdominal withdrawal reflex (AWR) scores were assessed by colorectal distension; protein and mRNA expression of PK2 and PKR2 in rat colon and spinal cord was determined by immunohistochemistry and fluorescence quantitative PCR. Compared with normal rats, the AWR scores of rats and the expressions of PK2/PKR2 proteins and mRNAs in colon and spinal cord tissue were significantly increased in the model group; compared with the model group, the AWR scores of rats and the expressions of PK2/PKR2 proteins and mRNAs in colon and spinal cord tissue were significantly decreased in the mild moxibustion group. These findings suggest that the analgesia effect of mild moxibustion may be associated with the reduction of the abnormally increased expression of the PK2/PKR2 proteins and mRNAs in the colon and spinal cord.

scholarly journals Effect of moxibustion on the expression of GDNF and its receptor GFRα3 in the colon and spinal cord of rats with irritable bowel syndrome

2019 ◽  
Vol 37 (4) ◽  
pp. 244-251 ◽  
Author(s):  
Qin Qi ◽  
Huangan Wu ◽  
Xiaoming Jin ◽  
Duiyin Jin ◽  
Yuanyuan Wang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Irritable Bowel Syndrome ◽  
Rat Model ◽  
Normal Group ◽  
Mustard Oil ◽  
Group Model ◽  
Model Group ◽  
Moxibustion Group ◽  
Mild Moxibustion ◽  
Irritable Bowel

Background: Moxibustion treatment has been found to ameliorate clinical symptoms including abdominal pain, diarrhoea and constipation in patients with irritable bowel syndrome (IBS). Herein we investigated the mechanisms underlying the use of moxibustion in a rat model of IBS. Methods: In our study, an IBS model was established in rats by colorectal distension (CRD) stimulus and mustard oil enema. The rats were randomly divided into a normal group, model group, mild moxibustion group, electroacupuncture group, probiotic group and dicetel group. Abdominal withdrawal reflex (AWR) scores were determined within 90 min of the last treatment. The expression of GDNF/GFRα3 protein and mRNA in the colon and spinal cord were detected by immunohistochemistry and quantitative real-time-PCR, respectively. Results: The IBS model rats had significantly higher AWR scores than the normal group ( P<0.01). After mild moxibustion treatment, the AWR score was significantly reduced (20 mm Hg, P<0.05; 40 mm Hg, 60 mm Hg and 80 mm Hg, P<0.01). The model group showed significantly more colonic glial cell line-derived neurotrophic factor (GDNF/GFRα3 (GDNF family receptor α3) protein and mRNA expression in the colon and spinal cord than the normal group ( P<0.01). Compared with the model group, the expression of GDNF/GFRα3 protein and mRNA in the colon and spinal cord of the rats were significantly decreased in the mild moxibustion group (colon: GDNF and GFRα3 protein, P<0.01; GDNF and GFRα3 mRNA, P<0.01; spinal cord: GDNF and GFRα3 protein, P<0.01; GDNF mRNA, P<0.05, GFRα3 mRNA, P<0.01). Conclusions: Our data suggest that moxibustion therapy may mitigate CRD-induced increases in the expression of GDNF and its receptor GFRα3 in the colon and spinal cord in a rat model of IBS.

Strain-dependent effects of probiotics on intestinal muscle dysfunction in an animal model of post-infective irritable bowel syndrome

2003 ◽  
Vol 124 (4) ◽  
pp. A29 ◽  
Author(s):  
Elena F. Verdu ◽  
Premysl Bercik ◽  
Patricia Blennerhassett ◽  
Xian Xi Huang ◽  
Gabriela Bergonzelli ◽  
...  
Keyword(s):  
Animal Model ◽  
Irritable Bowel Syndrome ◽  
Muscle Dysfunction ◽  
Irritable Bowel ◽  
Strain Dependent

scholarly journals Study on the Mechanism Underlying the Regulation of the NMDA Receptor Pathway in Spinal Dorsal Horns of Visceral Hypersensitivity Rats by Moxibustion

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
L. D. Wang ◽  
J. M. Zhao ◽  
R. J. Huang ◽  
L. Y. Tan ◽  
Z. H. Hu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Irritable Bowel Syndrome ◽  
Visceral Pain ◽  
Intrathecal Injection ◽  
Visceral Hypersensitivity ◽  
Regulatory Function ◽  
Visceral Hyperalgesia ◽  
Spinal Dorsal ◽  
Dorsal Horns ◽  
Irritable Bowel

Visceral hypersensitivity is enhanced in irritable bowel syndrome (IBS) patients. Treatment of IBS visceral pain by moxibustion methods has a long history and rich clinical experience. In the clinic, moxibustion on the Tianshu (ST25) and Shangjuxu (ST37) acupoints can effectively treat bowel disease with visceral pain and diarrhea symptoms. To investigate the regulatory function of moxibustion on the Tianshu (ST25) and Shangjuxu (ST37) acupoints on spinal cord NR1, NR2B, and PKCεprotein and mRNA expression in irritable bowel syndrome (IBS) visceral hypersensitivity rats, we did some research. In the study, we found that moxibustion effectively relieved the IBS visceral hyperalgesia status of rats. Analgesic effect of moxibustion was similar to intrathecal injection of Ro 25-6981. The expression of NR1, NR2B, and PKCεin the spinal dorsal horns of IBS visceral hyperalgesia rats increased. Moxibustion on the Tianshu and Shangjuxu acupoints might inhibit the visceral hypersensitivity, simultaneously decreasing the expression of NR1, NR2B, and PKCεin spinal cord of IBS visceral hyperalgesia rats. Based on the above experimental results, we hypothesized NR1, NR2B, and PKCεof spinal cord could play an important role in moxibustion inhibiting the process of central sensitization and visceral hyperalgesia state.

scholarly journals Effect of Electroacupuncture on Visceral Hyperalgesia, Serotonin and Fos Expression in an Animal Model of Irritable Bowel Syndrome

2010 ◽  
Vol 16 (3) ◽  
pp. 306-314 ◽  
Author(s):  
Justin CY Wu ◽  
Eric TC Ziea ◽  
Lixing Lao ◽  
Emma FC Lam ◽  
Catherine SM Chan ◽  
...  
Keyword(s):  
Animal Model ◽  
Irritable Bowel Syndrome ◽  
Visceral Hyperalgesia ◽  
Fos Expression ◽  
Irritable Bowel

scholarly journals Simmering Innards: Does Irritable Bowl Syndrome Have an Immunological Basis?

2004 ◽  
Vol 18 (10) ◽  
pp. 601-603 ◽  
Author(s):  
Christopher N Andrews ◽  
Eldon A Shaffer
Keyword(s):  
Spinal Cord ◽  
Irritable Bowel Syndrome ◽  
Low Grade ◽  
Irritable Bowel ◽  
Low Grade Inflammation ◽  
Brain And Spinal Cord ◽  
The Brain

Not so long ago, physicians construed the irritable bowel syndrome (IBS) as being a neurotic trait: it was all in the head. Today most clinicians believe that the main abnormality lies in the brain (and spinal cord), which reacts abnormally to stimuli from the gut. Recent studies are identifying a basis for these neural changes - low grade inflammation in the gut - which may play a key role in IBS.

Effect ofLactobacillus plantarum299v treatment in an animal model of irritable bowel syndrome

2009 ◽  
Vol 21 (1) ◽  
pp. 33-37 ◽  
Author(s):  
Alistair W.G. Waugh ◽  
Rae Foshaug ◽  
Sarah Macfarlane ◽  
Jason SG Doyle ◽  
Thomas A. Churchill ◽  
...  
Keyword(s):  
Animal Model ◽  
Irritable Bowel Syndrome ◽  
Irritable Bowel
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