Mechanisms of tolerance induction after rat liver transplantation: Intrahepatic CD4+T cells produce different cytokines during rejection and tolerance in response to stimulation

Abstract Background Orthotopic rat liver transplantation (OLT) is widely used; it remains to be challenging although many renovations have been made. Methods For tolerance induction, we performed OLT including whole graft OLT from close Sprague Dawley (SD) rat to SD (whole graft group, control group, n = 21) and 30 minute anhepatic time (AHT) group (AHT group, n = 11). For good outcomes of AHT group and generalization of this maneuver, partial OLT was performed from 50% Lewis allograft to Brown Norway (BN) rats to induce tolerance (half graft group, n = 28), Cyclosporine A was injected once daily for 14 days. Results For whole graft group, 30-day survival rate was 85.5% (18/21), the reasons of death were gas embolism due to the missed suturing in 2 cases, blood loss in 1 case. For AHT group and tolerance group, 30-day survival rate was 72.7% (8/11), 64.3% (18/28), the causes of death were diverse. There were no differences in survival in 3 groups (p = 0.289). Conclusion The anhepatic time can be extended simply through the change of clamping the diaphragm, which facilitates its application in the research.

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The Simple Maneuver to Safely Extend the Anhepatic Time to 30 Minutes for Rat Liver Transplantation

10.21203/rs.3.rs-118171/v1 ◽

2020 ◽

Author(s):

Gaofeng Tang ◽

Jiabin Zhang ◽

Huibo Zhao ◽

Sidong Wei ◽

shaotang zhou ◽

...

Keyword(s):

Liver Transplantation ◽

Survival Rate ◽

Rat Liver ◽

Tolerance Induction ◽

Brown Norway ◽

Control Group ◽

Sprague Dawley ◽

Once Daily ◽

Rat Liver Transplantation ◽

Group Control Group

Abstract Background: Orthotopic rat liver transplantation (OLT) is widely used; it remains to be challenging although many renovations have been made. Methods: For tolerance induction, we performed OLT including whole graft OLT from close Sprague Dawley (SD) rat to SD (whole graft group, control group, n=21) and 30 minute anhepatic time (AHT) group (AHT group, n=11). For good outcomes of AHT group and generalization of this maneuver, partial OLT was performed from 50% Lewis allograft to Brown Norway (BN) rats to induce tolerance (half graft group, n=28), Cyclosporine A was injected once daily for 14 days. Results: For whole graft group, 30-day survival rate was 85.5% (18/21), the reasons of death were gas embolism due to the missed suturing in 2 cases, blood loss in 1 case. For AHT group and tolerance group, 30-day survival rate was 72.7% (8/11), 64.3% (18/28), the causes of death were diverse. There were no differences in survival in 3 groups (p=0.289). Conclusion: The anhepatic time can be extended simply through the change of clamping the diaphragm, which facilitates its application in the research.

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The simple surgery to safely extend the anhepatic time to 30 minutes for rat liver transplantation

10.21203/rs.3.rs-38945/v1 ◽

2020 ◽

Author(s):

Gaofeng Tang ◽

Jiabin Zhang ◽

Huibo Zhao ◽

Sidong Wei ◽

shaotang Zhou ◽

...

Keyword(s):

Liver Transplantation ◽

Survival Rate ◽

Rat Liver ◽

Tolerance Induction ◽

Wound Dehiscence ◽

Brown Norway ◽

Sprague Dawley ◽

Once Daily ◽

Sd Rat ◽

Rat Liver Transplantation

Abstract Background Orthotopic rat liver transplantation (OLT) is widely used, it remains complicated although many renovations have been made. Methods OLT was performed for tolerance induction, including whole graft OLTs from close male Sprague Dawley (SD) rat to male SD (whole graft group, n = 21) and 30 minute anhepatic time group (AHT group, n = 11), OLT was performed from 50% male Lewis allograft to male Brown Norway (BN) rats to induce tolerance (half graft group, n = 28), Cyclosporine A was injected once daily for 14 days. Results For whole graft group, 30-day survival rate was 85.5% (18/21), the reasons of death were gas embolism due to the missed suturing in 2 cases, blood loss in 1 case. For AHT group, 30-day survival rate 72.7% (8/11), the causes of death were respiratory failure in 1 case, unknown in 2 cases. For tolerance group, 30-day survival rate was 64.3% (18/28), the reasons of death were diarrhea in 4 cases, wound dehiscence in 6 cases. There were no differences in survival in 3 groups (p = 0.289). Conclusion The anhepatic time can be extended simply through the change of clamping the diaphragm, which facilitates its application in the research.

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