Genes expressed in non-transformed human intestinal primary epithelial cell (HIPEC) lines derived from patients with IBD and controls

2001 ◽  
Vol 120 (5) ◽  
pp. A695-A696
Author(s):  
A JOSEPH ◽  
A PANJA
Keyword(s):  
Epithelial Cell ◽  
Primary Epithelial Cell

Establishment of primary epithelial cell culture from elutriated rat gastric mucosal cells

1995 ◽  
Vol 30 (2) ◽  
pp. 135-141 ◽  
Author(s):  
Yoshikazu Kinoshita ◽  
Sazzad Hassan ◽  
Hirohisa Nakata ◽  
Masakyo Asahara ◽  
Yumi Matsushima ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Epithelial Cell Culture ◽  
Gastric Mucosal Cells ◽  
Primary Epithelial Cell ◽  
Mucosal Cells

Primary epithelial cell culture of adult rat kidney, enhancement of cell growth by ammonium acetate

1979 ◽  
Vol 121 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Jules Berman ◽  
Alan Perantoni ◽  
Hester Marie Jackson ◽  
Elizabeth Kingsbury
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Epithelial Cell ◽  
Ammonium Acetate ◽  
Rat Kidney ◽  
Epithelial Cell Culture ◽  
Adult Rat ◽  
Primary Epithelial Cell

Passive siRNA transfection method for gene knockdown in air-liquid interface airway epithelial cell cultures

2021 ◽  
Author(s):  
Colleen M Bartman ◽  
Kimberly E Stelzig ◽  
David R Linden ◽  
Y. S. Prakash ◽  
Sergio E Chiarella
Keyword(s):  
Epithelial Cell ◽  
Liquid Interface ◽  
Airway Epithelial ◽  
Loss Of Function ◽  
Sirna Transfection ◽  
Simple Method ◽  
Primary Epithelial Cell ◽  
Transfection Protocol ◽  
Air Liquid Interface

Differentiation of human bronchial epithelial cells (HBEs) in air-liquid interface (ALI) cultures recapitulates organotypic modeling of the in vivo environment. Although ALI cultures are invaluable for studying the respiratory epithelial barrier, loss-of-function studies are limited by potentially cytotoxic reagents in classical transfection methods, the length of the differentiation protocol, and the number of primary epithelial cell passages. Here, we present the efficacy and utility of a simple method for siRNA transfection of HBEs in ALI cultures that does not require potentially cytotoxic transfection reagents and does not detrimentally alter the physiology of HBEs during the differentiation process. This transfection protocol introduces a reproducible and efficient method for loss-of-function studies in HBE ALI cultures that can be leveraged for modeling the respiratory system and airway diseases.

scholarly journals Method for Primary Epithelial Cell Culture from the Rat Choroid Plexus

BIO-PROTOCOL ◽  
2020 ◽  
Vol 10 (4) ◽  
Author(s):  
Valeria Lallai ◽  
Amina Ahmed ◽  
Christie Fowler
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Choroid Plexus ◽  
Epithelial Cell Culture ◽  
Primary Epithelial Cell

An improved and easy protocol for primary epithelial cell culture from atretic tissue in biliary atresia

2019 ◽  
Vol 56 ◽  
pp. 83-89
Author(s):  
Sanghamitra Machhua ◽  
Yashwant Kumar ◽  
Ravi Prakash Kanojia ◽  
Alka Bhatia ◽  
Babu Ram Thapa ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Biliary Atresia ◽  
Epithelial Cell Culture ◽  
Primary Epithelial Cell

Primary epithelial cell cultures derived from canine prostate: Isolation, culture, and characterization

1982 ◽  
Vol 164 (4) ◽  
pp. 311-332 ◽  
Author(s):  
Louis Terracio ◽  
William H. J. Douglas ◽  
Dominic Pennachio ◽  
Richard L. Vena ◽  
Peter Ofner
Keyword(s):  
Epithelial Cell ◽  
Cell Cultures ◽  
Epithelial Cell Cultures ◽  
Primary Epithelial Cell

Cytokeratin expression in primary epithelial cell culture from bovine conjunctiva

2004 ◽  
Vol 36 (5) ◽  
pp. 323-332 ◽  
Author(s):  
G. Paladino ◽  
C. Marino ◽  
S. La Terra Mulè ◽  
C. Civiale ◽  
D. Rusciano ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Epithelial Cell Culture ◽  
Primary Epithelial Cell ◽  
Cytokeratin Expression
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