Su2001 Altered Expression and Activation of the CXCR3/CXCL10 Chemokine System in Irritable Bowel Syndrome (IBS) Mucosal Biopsy Tissue

2012 ◽  
Vol 142 (5) ◽  
pp. S-557
Author(s):  
Gerard Moloney ◽  
Aine Fanning ◽  
John Mac Sharry ◽  
Lindsay J. Hall ◽  
Fergus Shanahan ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Mucosal Biopsy ◽  
Altered Expression ◽  
Biopsy Tissue ◽  
Irritable Bowel

scholarly journals Stimulation of T-Cells in Irritable Bowel Syndrome (IBS) Mucosal Biopsy Tissue Releases Cytokines Which Selectively Activate Submucosal Neurons

2011 ◽  
Vol 140 (5) ◽  
pp. S-129
Author(s):  
Gerard Moloney ◽  
Aine Fanning ◽  
Dervla O'Malley ◽  
Niamh Scanlan ◽  
John F. Cryan ◽  
...  
Keyword(s):  
T Cells ◽  
Irritable Bowel Syndrome ◽  
Mucosal Biopsy ◽  
Biopsy Tissue ◽  
Irritable Bowel ◽  
Stimulation Of

390 Impairment of Human Colonic Smooth Muscle Contractility Following Exposure to Mucosal Biopsy Supernatants Obtained From Irritable Bowel Syndrome Patients

2014 ◽  
Vol 146 (5) ◽  
pp. S-83
Author(s):  
Michele Pier Luca Guarino ◽  
Giovanni Barbara ◽  
Annamaria Altomare ◽  
M. Raffaella Barbaro ◽  
Silvia Cocca ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Smooth Muscle ◽  
Mucosal Biopsy ◽  
Muscle Contractility ◽  
Smooth Muscle Contractility ◽  
Irritable Bowel

Submucous rather than myenteric neurons are activated by mucosal biopsy supernatants from irritable bowel syndrome patients

2012 ◽  
Vol 24 (12) ◽  
pp. 1134-e572 ◽  
Author(s):  
S. Buhner ◽  
Q. Li ◽  
T. Berger ◽  
S. Vignali ◽  
G. Barbara ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Mucosal Biopsy ◽  
Myenteric Neurons ◽  
Irritable Bowel

Neuronal activation by mucosal biopsy supernatants from irritable bowel syndrome patients is linked to visceral sensitivity

2014 ◽  
Vol 99 (10) ◽  
pp. 1299-1311 ◽  
Author(s):  
Sabine Buhner ◽  
Breg Braak ◽  
Qin Li ◽  
Eva Maria Kugler ◽  
Tamira Klooker ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Mucosal Biopsy ◽  
Neuronal Activation ◽  
Visceral Sensitivity ◽  
Irritable Bowel

Regulation of serum microRNA expression by acupuncture in patients with diarrhea-predominant irritable bowel syndrome

2021 ◽  
pp. 096452842110278
Author(s):  
Jing Guo ◽  
Gao Lu ◽  
Lu Chen ◽  
Hao Geng ◽  
Xiaoliang Wu ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Abdominal Pain ◽  
Pcr Analysis ◽  
Altered Expression ◽  
Qrt Pcr ◽  
Serum Microrna ◽  
Symptom Severity Scale ◽  
Before And After ◽  
Irritable Bowel ◽  
Healthy Participants

Objective: To screen for differentially expressed serum microRNAs (miRNAs) in patients with diarrhea-predominant irritable bowel syndrome (IBS-D) compared with healthy participants and explore the mechanism of acupuncture in the treatment of IBS-D based on miRNAs. Methods: IBS-D patients that met the Rome III diagnostic criteria and age- and sex-matched healthy participants were enrolled between April 2017 and December 2017. Serum miRNA levels were initially determined using a TaqMan low-density array (TLDA) in pooled samples. Markedly altered miRNAs in IBS-D patients were subsequently validated using quantitative real-time polymerase chain reaction (qRT-PCR) on individual samples. All IBS-D patients accepted the acupuncture therapy for 6 weeks. The disease severity was assessed using the IBS symptom severity scale (IBS-SSS) questionnaire before and after treatment. After acupuncture, the patients’ serum was re-analyzed for altered expression of the miRNAs by qRT-PCR. Results: TLDA and qRT-PCR analysis revealed six upregulated miRNAs (miR-1305, miR-575, miR-149-5p, miR-190a-5p, miR-135a-5p, and miR-148a-3p; P < 0.05) and two downregulated miRNAs (miR-194-5p, miR-127-5p; P < 0.05) in IBS-D patients compared with healthy controls. Post acupuncture treatment, total IBS-SSS scores, severity of abdominal pain, duration of abdominal pain, severity of abdominal distention, dissatisfaction with bowel habits and disruption in quality of life decreased significantly ( P < 0.001). Furthermore, the upregulated miR-148a-3p levels in IBS-D patients also decreased significantly after acupuncture ( P < 0.05). Conclusions: The over-expression or reduced expression of several miRNAs may contribute to IBS-D pathogenesis. Acupuncture might downregulate miR-148a-3p through multiple pathways to alleviate or relieve IBS-D symptoms. Trial registration number: ChiCTR-IOR-17010860 (Chinese Clinical Trials Registry)

scholarly journals Alterations in Expression of p11 and SERT in Mucosal Biopsy Specimens of Patients With Irritable Bowel Syndrome

2007 ◽  
Vol 132 (1) ◽  
pp. 17-25 ◽  
Author(s):  
Michael Camilleri ◽  
Christopher N. Andrews ◽  
Adil E. Bharucha ◽  
Paula J. Carlson ◽  
Irene Ferber ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Mucosal Biopsy ◽  
Biopsy Specimens ◽  
Irritable Bowel

scholarly journals Increased Expression of Colonic Mucosal Melatonin in Patients with Irritable Bowel Syndrome Correlated with Gut Dysbiosis

2020 ◽  
Author(s):  
Ben Wang ◽  
Shiwei Zhu ◽  
Zuojing Liu ◽  
Hui Wei ◽  
Lu Zhang ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Gut Microbiota ◽  
Fecal Microbiota ◽  
Protective Mechanism ◽  
Altered Expression ◽  
Promising Target ◽  
Gut Hormone ◽  
Rdna Sequencing ◽  
Irritable Bowel ◽  
Butyrate Production

AbstractDysregulation of the gut microbiota/gut hormone axis contributes to the pathogenesis of irritable bowel syndrome (IBS). Melatonin plays a beneficial role in gut motility and immunity. However, altered expression of local mucosal melatonin in IBS and its relationship with the gut microbiota remain unclear. Therefore, we aimed to detect the colonic melatonin levels and microbiota profiles in patients with diarrhea-predominant IBS (IBS-D) and explore their relationship in germ-free (GF) rats and BON-1 cells. Thirty-two IBS-D patients and twenty-eight healthy controls (HC) were recruited. Fecal specimens from IBS-D patients and HCs were separately transplanted into GF rats by gavage. The levels of colon mucosal melatonin were assessed by immunohistochemical methods, and fecal microbiota communities were analyzed using 16S rDNA sequencing. The effect of butyrate on melatonin synthesis in BON-1 cells was evaluated by ELISA. Melatonin levels were significantly increased in IBS-D patients compared with HC and were significantly negatively correlated with visceral sensitivity in IBS-D patients. GF rats inoculated with fecal microbiota from IBS-D patients had high colonic melatonin levels. Butyrate-producing Clostridium cluster XIVa species, such as Roseburia species and Lachnospira species, were positively related to colonic mucosal melatonin expression. Butyrate significantly increased melatonin secretion in BON-1 cells. Increased melatonin expression may be an adaptive protective mechanism in the development of IBS-D. Moreover, some Clostridium cluster XIVa species could increase melatonin expression via butyrate production. Modulation of the gut hormone/gut microbiota axis offers a promising target of interest for IBS in the future.

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