scholarly journals Stimulation of T-Cells in Irritable Bowel Syndrome (IBS) Mucosal Biopsy Tissue Releases Cytokines Which Selectively Activate Submucosal Neurons

2011 ◽  
Vol 140 (5) ◽  
pp. S-129
Author(s):  
Gerard Moloney ◽  
Aine Fanning ◽  
Dervla O'Malley ◽  
Niamh Scanlan ◽  
John F. Cryan ◽  
...  
Keyword(s):  
T Cells ◽  
Irritable Bowel Syndrome ◽  
Mucosal Biopsy ◽  
Biopsy Tissue ◽  
Irritable Bowel ◽  
Stimulation Of

Su2001 Altered Expression and Activation of the CXCR3/CXCL10 Chemokine System in Irritable Bowel Syndrome (IBS) Mucosal Biopsy Tissue

2012 ◽  
Vol 142 (5) ◽  
pp. S-557
Author(s):  
Gerard Moloney ◽  
Aine Fanning ◽  
John Mac Sharry ◽  
Lindsay J. Hall ◽  
Fergus Shanahan ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Mucosal Biopsy ◽  
Altered Expression ◽  
Biopsy Tissue ◽  
Irritable Bowel

390 Impairment of Human Colonic Smooth Muscle Contractility Following Exposure to Mucosal Biopsy Supernatants Obtained From Irritable Bowel Syndrome Patients

2014 ◽  
Vol 146 (5) ◽  
pp. S-83
Author(s):  
Michele Pier Luca Guarino ◽  
Giovanni Barbara ◽  
Annamaria Altomare ◽  
M. Raffaella Barbaro ◽  
Silvia Cocca ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Smooth Muscle ◽  
Mucosal Biopsy ◽  
Muscle Contractility ◽  
Smooth Muscle Contractility ◽  
Irritable Bowel

Submucous rather than myenteric neurons are activated by mucosal biopsy supernatants from irritable bowel syndrome patients

2012 ◽  
Vol 24 (12) ◽  
pp. 1134-e572 ◽  
Author(s):  
S. Buhner ◽  
Q. Li ◽  
T. Berger ◽  
S. Vignali ◽  
G. Barbara ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Mucosal Biopsy ◽  
Myenteric Neurons ◽  
Irritable Bowel

scholarly journals Increased Vδ1γδT cells Predominantly Contributed to IL-17 Production in the Development of Adult Human Post-infectious Irritable Bowel Syndrome

2020 ◽  
Author(s):  
liwei dong ◽  
xiaoning sun ◽  
zhichao ma ◽  
jiao fu ◽  
fujin liu ◽  
...  
Keyword(s):  
T Cells ◽  
Irritable Bowel Syndrome ◽  
Peripheral Blood ◽  
Γδ T Cells ◽  
Γδt Cells ◽  
Peripheral T Cells ◽  
Confocal Fluorescence ◽  
Irritable Bowel ◽  
Post Infectious

Abstract Background: γδT cells play an important role in the mucosa inflammation and immunity-associated disorders. Our previous study reported that γδT cells producing IL-17 were involved in the pathogenesis of post-infectious irritable bowel syndrome (PI-IBS). However, their subset characteristic profile in this kind of disease remains unclear. Thus the current study’s aim is to investigate the functionally predominant subset and its role in PI-IBS. Methods: The total T cells were collected from the peripheral blood of patients with PI-IBS. The peripheral proportion of Vδ1 and Vδ2 subset was detected by FACS after stained with anti δ1-PE and anti δ2-APC. The local colonic proportion of this two subsets were measured under laser confocal fluorescence microscope. Vδ1 γδ T cells were enriched from the total peripheral T cells by minoantibody-immuno-microbeads (MACS) method. and cultured, functionally evaluated by CCK-8 assay (proliferation)，CD69/CD62L molecules expression assay (activation) and ELISA (IL-17 production) respectively.Results: 1. Vδ1 γδ T cells significantly increased while Vδ2 γδ T cells remained unchanged in both the peripheral blood and local colonic tissue from PI-IBS patients (P<0.05). 2. When cultured in vitro, the Vδ1 γδ T cells remarkably proliferated, activated and produced IL-17 (P<0.05). Conclusions: Our results suggest that Vδ1 γδ T cells was the predominant γδ T cells subset in both peripheral and intestinal tissue, and was the major IL-17 producing γδ T cells in PI-IBS.

scholarly journals Quantitation of Colonic Cells as Severity Markers in Patients with Irritable Bowel Syndrome

2018 ◽  
Vol 7 ◽  
pp. e1063
Author(s):  
Mohammad Bagher Miri ◽  
Amir Sadeghi ◽  
Afshin Moradi ◽  
Mohammad Rostami-Nejad ◽  
Mohammad-Javad Ehsani-Ardekani ◽  
...  
Keyword(s):  
T Cells ◽  
Irritable Bowel Syndrome ◽  
Mast Cells ◽  
Inflammatory Cells ◽  
Normal Subjects ◽  
Intraepithelial Lymphocytes ◽  
Control Group ◽  
Case Group ◽  
Cross Sectional ◽  
Irritable Bowel

Background: Irritable bowel syndrome (IBS) is the most common gastrointestinal syndrome. Routine histopathology and immunohistochemistry (IHC) evaluations have shown an increase in the number of different inflammatory cells in the colon of IBS patients. In this study, we have compared the number of intraepithelial lymphocytes (IELs), eosinophils, mast cells and CD3+ T cells, in IBS patients and normal subjects. Materials and Methods: In 2016, seventy-nine patients with IBS and seventy-nine healthy subjects who underwent colonoscopy for other non-specific causes and with no pathologic findings, were enrolled in this cross-sectional study. Biopsy specimens obtained from the colon were stained, using IHC methods to determine the number of IELs, eosinophils, mast cells and CD3+ T cells. Quantitative and qualitative variables were compared between the two groups, using a Chi-square test and Student’s t-test. Results: Seventy-nine patients with IBS, 79.7% females with a mean age of 42.5±14.6 years, were recruited, as the case group, and seventy-nine individuals, 51.9% females with a mean age of 39.7±18.9 years, were enrolled as controls. The average number of IELs per high power fields (hpf) was found to be higher in the IBS group, and this difference was statistically significant (32.8±11.8 vs. 28.6±12.9; P=0.034). Also, the mean count/hpf of CD3+ T lymphocytes (23.1±7.9 vs. 20.2±8.1; P=0.024) and mast cells (7.6±3.1 vs. 6.6±3.0; P=0.041) were significantly higher in the IBS group, compared to the control group. The number of eosinophils was higher in the IBS group, but the differences were not statistically significant (P=0.066). Conclusion: According to the results, we suggest that analysis of immune cells and IELs in intestinal biopsies might be an appropriate method for diagnosis of IBS. [GMJ.2018;7:e1063]

scholarly journals Increased Vδ1 γδT cells Predominantly Contributed to IL-17 Production in the Development of Adult Human Post-infectious Irritable Bowel Syndrome

2020 ◽  
Author(s):  
liwei dong ◽  
xiaoning sun ◽  
zhichao ma ◽  
jiao fu ◽  
fujin liu ◽  
...  
Keyword(s):  
T Cells ◽  
Irritable Bowel Syndrome ◽  
Peripheral Blood ◽  
Γδ T Cells ◽  
Γδt Cells ◽  
Peripheral T Cells ◽  
Confocal Fluorescence ◽  
Irritable Bowel ◽  
Post Infectious

Abstract Background: γδT cells play an important role in the mucosa inflammation and immunity-associated disorders. Our previous study reported that γδT cells producing IL-17 were involved in the pathogenesis of post-infectious irritable bowel syndrome (PI-IBS). However, their subset characteristic profile in this kind of disease remains unclear. Thus the current study’s aim is to investigate the functionally predominant subset and its role in PI-IBS. Methods: The total T cells were collected from the peripheral blood of patients with PI-IBS. The peripheral proportion of Vδ1 and Vδ2 subset was detected by FACS after stained with anti δ1-PE and anti δ2-APC. The local colonic proportion of this two subsets were measured under laser confocal fluorescence microscope. Vδ1 γδT cells were enriched from the total peripheral T cells by minoantibody-immuno-microbeads (MACS) method. and cultured, functionally evaluated by CCK-8 assay (proliferation)，CD69/CD62L molecules expression assay (activation) and ELISA (IL-17 production) respectively. Results: 1. Vδ1 γδ T cells significantly increased while Vδ2 gd T cells remained unchanged in both the peripheral blood and local colonic tissue from PI-IBS patients (P<0.05). 2. When cultured in vitro, the Vδ1 gd T cells remarkably proliferated, activated and produced IL-17 (P<0.05）. Conclusions: Our results suggest that Vδ1 γδ T cells was the predominant γδ T cells subset in both peripheral and intestinal tissue, and was the major IL-17 producing γδ T cells in PI-IBS.

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