The stimulated contractile function of aerobic isolated adult rat heart cells was assessed by laser light diffraction. Cells were maintained for up to 8 h by attachment to a cover slip and continuous perfusion in a chamber on the microscope stage. On stimulation such cells beat as though unattached. Cells showed a negative staircase which was reduced by increasing Ca or isoproterenol. Ryanodine caused a positive staircase on stimulation, which was enhanced by increasing Ca or isoproterenol or by using cells from younger rats. For beats of constant contraction duration, there was a linear relationship between the magnitude of cell shortening and the velocity of shortening, independent of the concentration of extracellular Ca. We conclude the following. 1) The beat characteristics of isolated cells are very similar to those of papillary muscle. 2) Attachment of cells need not alter their unloaded shortening characteristics. 3) Cells appear to contract against an internal load, to an extent determined by the degree of myofilament activation. 4) Cells from young rats require less extracellular Ca than those from adult rats for the same beat magnitude.
Changes in cytosolic free calcium induced by metabolic inhibitors or hypoxia in isolated adult rat heart cells
