A deoxyribonucleic acid unwinding protein isolated from regenerating rat liver. Physical and functional properties.

Deoxycytidylate deaminase activity and net synthesis of deoxyribonucleic acid (DNA) in vivo were found to increase at approximately the same time during the early stages of liver regeneration. However, deaminase activity in the regenerating liver remained at a high level for 1 day after DNA synthesis had slowed down again during the later stages of regeneration. The increase in deaminase activity was restricted as a result of exposure to 600 r X radiation during early regeneration, but this effect only became evident 11–16 hours after the irradiation. Irradiation on the second day after partial hepatectomy, when deaminase levels in control regenerating livers were relatively constant, failed to affect the deaminase activity immediately but did produce a 40–50% decrease in activity 11–16 hours later. Other antimitotic agents, e.g., colchicine, had little effect on deaminase activity.

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DEOXYRIBONUCLEIC ACID SYNTHESIS IN REGENERATING RAT LIVER STUDIED BY CYTOFLUOROMETRY IN COMBINATION WITH TRITIATED THYMIDINE AUTORADIOGRAPHY

ACTA HISTOCHEMICA ET CYTOCHEMICA ◽

10.1267/ahc.7.105 ◽

1974 ◽

Vol 7 (2) ◽

pp. 105-111 ◽

Cited By ~ 3

Author(s):

SETSUYA FUJITA ◽

TSUKASA ASHIHARA ◽

MASARU FUKUDA ◽

OSAMU TAKEOKA

Keyword(s):

Rat Liver ◽

Deoxyribonucleic Acid ◽

Tritiated Thymidine ◽

Acid Synthesis ◽

Regenerating Rat Liver ◽

Deoxyribonucleic Acid Synthesis ◽

Thymidine Autoradiography

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Alterations in Deoxyribonucleic Acid from Regenerating Rat Liver after X-radiation in vivo

Nature ◽

10.1038/194883a0 ◽

1962 ◽

Vol 194 (4831) ◽

pp. 883-884 ◽

Cited By ~ 5

Author(s):

R. FOSTER ◽

MARGERY G. ORD

Keyword(s):

Rat Liver ◽

Deoxyribonucleic Acid ◽

Regenerating Rat Liver

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Inhibition of polyamine accumulation and deoxyribonucleic acid synthesis in regenerating rat liver

Biochemical Journal ◽

10.1042/bj1580485 ◽

1976 ◽

Vol 158 (2) ◽

pp. 485-488 ◽

Cited By ~ 58

Author(s):

H Pösö ◽

J Jänne

Keyword(s):

Partial Hepatectomy ◽

Ornithine Decarboxylase ◽

Rat Liver ◽

Deoxyribonucleic Acid ◽

Acid Synthesis ◽

Liver Protein ◽

Polyamine Synthesis ◽

Total Liver ◽

Regenerating Rat Liver ◽

Deoxyribonucleic Acid Synthesis

Repeated injections of 1,3-diaminopropane into rats after partial hepatectomy caused a repression-type inhibiton of liver ornithine decarboxylase (EC 4.1.1.17) and totally prevented the marked increases in liver putrescine and spermidine concentrations that normally occur in response to partial hepatectomy. The inhibition of polyamine synthesis by diaminopropane was accompanied by a profound decrease (about 80%) in the synthesis of DNA in the regenerating rat liver without any changes in the synthesis of RNA and total liver protein.

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