thymidine autoradiography
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1992 ◽  
Vol 262 (6) ◽  
pp. G1041-G1046 ◽  
Author(s):  
M. G. Blennerhassett ◽  
P. Vignjevic ◽  
D. L. Vermillion ◽  
S. M. Collins

Inflammation of the rat jejunum with Trichinella spiralis causes altered smooth muscle contractility by day 6 postinfection (PI). We investigated the association of structural change in the smooth muscle layers with inflammation. By day 6 PI, smooth muscle area in cross sections of jejunum increased (P less than 0.05) in longitudinal (LM) and circular (CM) muscle layers. Nuclei counting in cross sections showed that cell number increased two- to threefold in CM and LM, and this increase was not reversed on day 23 PI. Estimation of cell size showed significant hypertrophy by day 6 PI in both muscle layers. [3H]thymidine autoradiography showed that the labeling index (LI) of jejunal LM and CM increased sharply on day 4 PI and peaked on day 6 PI (10- to 15-fold increase). The noninflamed ileum showed a smaller trophic response, with no significant change in area or nuclei number, the LI was increased only on day 6 PI in the ileal CM and was unchanged in LM. Thus extensive hyperplasia and hypertrophy of smooth muscle cells are associated with intestinal inflammation.



1992 ◽  
Vol 175 (5) ◽  
pp. 1157-1167 ◽  
Author(s):  
K Inaba ◽  
R M Steinman ◽  
M W Pack ◽  
H Aya ◽  
M Inaba ◽  
...  

While it has been known that dendritic cells arise from proliferating precursors in situ, it has been difficult to identify progenitors in culture. We find that aggregates of growing dendritic cells develop in cultures of mouse blood that are supplemented with granulocyte/macrophage colony-stimulating factor (GM-CSF) but not other CSFs. The dendritic cell precursor derives from the Ia-negative and nonadherent fraction. The aggregates of developing dendritic cells appear at about 1 wk of culture, with 100 or more such clusters being formed per 10(6) blood leukocytes. The aggregates can be dislodged and subcultured as expanding clusters that are covered with cells having the motile sheet-like processes ("veils") of dendritic cells. By about 2 wk, large numbers of single, major histocompatibility complex (MHC) class II-rich dendritic cells begin to be released into the medium. Combined immunoperoxidase and [3H]thymidine autoradiography show that the cells that proliferate within the aggregate lack certain antigenic markers that are found on mature dendritic cells. However, in pulse-chase protocols, the [3H]thymidine-labeled progeny exhibit many typical dendritic cell features, including abundant MHC class II and a cytoplasmic granular antigen identified by monoclonal antibody 2A1. The progeny dendritic cells are potent stimulators of the mixed leukocyte reaction and can home to the T-dependent areas of lymph node after injection into the footpads. We conclude that mouse blood contains GM-CSF-dependent, proliferating progenitors that give rise to large numbers of dendritic cells with characteristic morphology, mobility, phenotype, and strong T cell stimulatory function.





1991 ◽  
Vol 6 (4) ◽  
pp. 383-391 ◽  
Author(s):  
Danru Zhang ◽  
Hermes H. Yeh

AbstractThis study describes a phenomenon of transient expression of corticotropin releasing factor-like immunoreactivity (CRF-LI) in immature horizontal cells of the developing rat retina. These cells could be distinguished from those destined to become CRF-LI amacrine cells in the adult by their location within the outer portion of the neuroblastic layer (NBL) and by their ontogenetic pattern. Upon initial detection on postnatal day 3 (PD-3), faint CRF-LI cellular profiles were found in the outer portion of the NBL, limited to the central region of the retina. Subsequently, on PD-5, these profiles began to appear in the periphery, forming a single horizontal row along the outermost aspect of the developing inner nuclear layer (INL), concomitant with the establishment of the outer plexiform layer (OPL). The results of our birth-dating study combining immunohistochemistry and [3H]-thymidine autoradiography indicated that these cells were generated between embryonic day 14 and 18. These findings are consistent with them being horizontal cells. Between PD-7 and PD-9, CRF-LI in horizontal cells began to diminish progressively following a center-to-periphery gradient such that only sporadic, faintly immunoreactive patches of cells could be seen by the time of eye opening (PD-15). Around PD-19, it declined to levels below immunohistochemical detection. However, when rats were reared in complete darkness beginning at birth until PD-21, the period of CRF-LI expression in horizontal cells was prolonged and persisted throughout the first three postnatal weeks of development.



1991 ◽  
Vol 260 (3) ◽  
pp. R468-R474
Author(s):  
M. Ochi ◽  
H. Yoshioka ◽  
T. Sawada ◽  
T. Kusunoki ◽  
T. Hattori

Cell kinetic characteristics of the epididymal adipose tissue of mice were examined during refeeding after prolonged food deprivation. Mature mice were given a glucose-electrolyte solution for 20-40 days, and subsequent to a body weight loss of 35%, they were given normal mouse food ad libitum. After refeeding, their body weight and adipose tissue weight returned to the levels before the deprivation. We examined the formation of new adipocytes in the epididymal adipose tissue with [3H]thymidine autoradiography. Flash labeling experiments revealed that cell proliferation was most active on the 6th day after refeeding and decreased thereafter until the 13th day when few labeled cells were seen. Cumulative labeling experiments showed that replicated poorly differentiated mesenchymal cells developed into adipocytes by storing fat droplets. New adipocyte formation was observed in adult mice during refeeding after long-term deprivation.



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