scholarly journals High guanine plus cytosine content in the third letter of codons of an extreme thermophile. DNA sequence of the isopropylmalate dehydrogenase of Thermus thermophilus.

1984 ◽  
Vol 259 (5) ◽  
pp. 2956-2960 ◽  
Author(s):  
Y Kagawa ◽  
H Nojima ◽  
N Nukiwa ◽  
M Ishizuka ◽  
T Nakajima ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Thermus Thermophilus ◽  
Extreme Thermophile ◽  
The Third ◽  
Isopropylmalate Dehydrogenase

Streptomycin-resistant and streptomycin-dependent mutants of the extreme thermophile Thermus thermophilus

2001 ◽  
Vol 309 (2) ◽  
pp. 333-338 ◽  
Author(s):  
Steven T Gregory ◽  
Jamie H.D Cate ◽  
Albert E Dahlberg
Keyword(s):  
Thermus Thermophilus ◽  
Extreme Thermophile

Ornithine Carbamoyltransferase from the Extreme Thermophile Thermus Thermophilus Analysis of the Gene and Characterisation of the Protein

1997 ◽  
Vol 248 (2) ◽  
pp. 466-474 ◽  
Author(s):  
Rony Sanchez ◽  
Margot Baetens ◽  
Mark Casteele ◽  
Martine Roovers ◽  
Christiane Legrain ◽  
...  
Keyword(s):  
Thermus Thermophilus ◽  
Ornithine Carbamoyltransferase ◽  
Extreme Thermophile

scholarly journals Temperature-Dependent Hypermutational Phenotype in recA Mutants of Thermus thermophilus HB27

2003 ◽  
Vol 185 (16) ◽  
pp. 4901-4907 ◽  
Author(s):  
Pablo Castán ◽  
Lorena Casares ◽  
Jordi Barbé ◽  
José Berenguer
Keyword(s):  
Mutation Rate ◽  
Deleterious Effect ◽  
Direct Relationship ◽  
Thermus Thermophilus ◽  
Mutation Rates ◽  
Gene Fragment ◽  
Extreme Thermophile ◽  
Temperature Dependent ◽  
Uv Sensitivity ◽  
Ampicillin Resistance Gene

ABSTRACT The recA gene from Thermus thermophilus HB27 was cloned and engineered to obtain insertion (recA::kat) and deletion (ΔrecA) derivatives. Transcription of recA in this extreme thermophile was induced by mitomycin C, leading to the synthesis of a monocistronic mRNA. This DNA damage-mediated induction was dependent on the integrity of recA. In addition to UV sensitivity, the recA mutants of T. thermophilus showed severe pleiotropic defects, ranging from irregular nucleoid condensation and segregation to a dramatic reduction in viability during culture. An increase in the frequency of both carotenoidless and auxotrophic mutants within surviving cells of the ΔrecA strain indicated a high mutation rate. As RecA is not required for plasmid transformation, we have used the α-lacZ gene fragment and the ampicillin resistance gene from Escherichia coli as passenger reporters to confirm such high mutation rates. Our data support the idea that the absence of RecA results in a hypermutational phenotype in T. thermophilus. Furthermore, a direct relationship is deduced between the growth temperature and mutation rate, which finally has a deleterious effect on cell survival in the absence of RecA.

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