scholarly journals Type I Procollagen N-proteinase from Chick Embryo Tendons

1989 ◽  
Vol 264 (19) ◽  
pp. 11336-11345 ◽  
Author(s):  
Y Hojima ◽  
J A McKenzie ◽  
M van der Rest ◽  
D J Prockop
Keyword(s):  
Chick Embryo ◽  
Type I ◽  
Type I Procollagen

scholarly journals Developmental changes in the type I procollagen processing pathway in chick-embryo cornea

1991 ◽  
Vol 276 (3) ◽  
pp. 777-784 ◽  
Author(s):  
S J Mellor ◽  
G L Atkins ◽  
D J S Hulmes
Keyword(s):  
Chick Embryo ◽  
Kinetic Model ◽  
Rate Constants ◽  
Collagen Fibril ◽  
Electrophoretic Analysis ◽  
Developmental Changes ◽  
Type I ◽  
Electron Microscopic ◽  
Type I Procollagen ◽  
Stage Of Development

Type I procollagen processing in chick-embryo corneas was studied at days 12, 14 and 17 of development. Pulse-chase experiments and electrophoretic analysis of salt-soluble extracts showed developmental changes in the processing pathway. A kinetic model was fitted to the data to determine rate constants for processing of both N- and C-propeptides. Data for pro alpha 1(I)-chain processing and pro alpha 2(I)-chain processing were fitted separately (where pro means procollagen). Between days 12 and 17 the relative flux through the pC-collagen (procollagen chain lacking the N-propeptide) and pN-collagen (procollagen chain lacking the C-propeptide) pathways increased approx. 4-fold. Pro alpha 1(I) chains and pro alpha 2(I) chains were processed by slightly different routes. Variations in the rate constants were compared with electron-microscopic measurements of collagen fibril diameters at each stage of development. Diameters increased by less than 10% over the period from 12 to 17 days. It was concluded that fibril diameters are relatively insensitive to the pathway of procollagen processing in the salt-soluble pool.

A Kinetic Analysis of Type I Procollagen Processing in Developing Chick Embryo Cornea

1990 ◽  
Vol 580 (1 Structure, Mo) ◽  
pp. 484-488
Author(s):  
SALLY J. MELLOR ◽  
GORDON L. ATKINS ◽  
DAVID J. S. HULMES
Keyword(s):  
Chick Embryo ◽  
Kinetic Analysis ◽  
Type I ◽  
Type I Procollagen
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