scholarly journals Isolation and characterization of the Saccharomyces cerevisiae MIS1 gene encoding mitochondrial C1-tetrahydrofolate synthase.

1988 ◽  
Vol 263 (16) ◽  
pp. 7717-7725 ◽  
Author(s):  
K W Shannon ◽  
J C Rabinowitz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Encoding ◽  
Isolation And Characterization ◽  
Tetrahydrofolate Synthase

scholarly journals Isolation and characterization of extragenic suppressors of mutations in the SSA hsp70 genes of Saccharomyces cerevisiae.

Genetics ◽  
1992 ◽  
Vol 131 (2) ◽  
pp. 277-285 ◽  
Author(s):  
R J Nelson ◽  
M F Heschl ◽  
E A Craig
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Stress Response ◽  
Regulatory Protein ◽  
Null Alleles ◽  
Temperature Sensitive ◽  
Gene Encoding ◽  
Isolation And Characterization ◽  
Transcriptional Regulatory ◽  
Extragenic Suppressors

Abstract Saccharomyces cerevisiae strains that contain null alleles of two hsp70 genes, SSA1 and SSA2, are temperature sensitive for growth. In this study, extragenic suppressors of ssa1 ssa2 have been isolated. Suppression is due to mutations at nuclear loci designated EXA1, EXA2 and EXA3 for EXtragenic suppressor hsp70 subfamily A. Two of the four EXA1 alleles are dominant as is EXA3-1. The other two EXA1 alleles as well as the sole EXA2 allele are recessive. EXA1 mutations lead to accumulation of a previously uncharacterized form of hsp70. EXA2 and EXA3 mutations affect the regulation of the stress response. In exa2-1 ssa1 ssa2 strains the gene products of the remaining SSA hsp70 genes, SSA3 and SSA4 (Ssa3/4p), accumulate to higher levels. The EXA3-1 mutation results in increased accumulation of both Ssa3/4p and the hsp70s encoded by the SSB1 and SSB2 genes (Ssb1/2p), suggesting that the EXA3 gene product plays a central role in the yeast stress response. Consistent with this hypothesis, EXA3-1 is tightly linked to HSF1, the gene encoding the transcriptional regulatory protein known as "heat shock factor." All of the genes identified in this study seem to be involved in regulating the expression of SSA3 and SSA4 or the activity of their protein products.

Isolation and Characterization of the Cryptococcus neoformans MATa Pheromone Gene

Genetics ◽  
2002 ◽  
Vol 160 (3) ◽  
pp. 935-947
Author(s):  
Carol M McClelland ◽  
Jianmin Fu ◽  
Gay L Woodlee ◽  
Tara S Seymour ◽  
Brian L Wickes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acids ◽  
Cryptococcus Neoformans ◽  
Mating Types ◽  
Filament Formation ◽  
Gene Encoding ◽  
Conserved Sequence ◽  
Isolation And Characterization ◽  
Conserved Genes

Abstract Cryptococcus neoformans is a heterothallic basidiomycete with two mating types, MATa and MATα. The mating pathway of this fungus has a number of conserved genes, including a MATα-specific pheromone (MFα1). A modified differential display strategy was used to identify a gene encoding the MATa pheromone. The gene, designated MFa1, is 42 amino acids in length and contains a conserved farnesylation motif. MFa1 is present in three linked copies that span a 20-kb fragment of MATa-specific DNA and maps to the MAT-containing chromosome. Transformation studies showed that MFa1 induced filament formation only in MATα cells, demonstrating that MFa1 is functionally conserved. Sequence analysis of the predicted Mfa1 and Mfα1 proteins revealed that, in contrast to other fungi such as Saccharomyces cerevisiae, the C. neoformans pheromone genes are structurally and functionally conserved. However, unlike the MFα1 gene, which is found in MATα strains of both varieties of C. neoformans, MFa1 is specific for the neoformans variety of C. neoformans.

Sign in / Sign up

Export Citation Format

Share Document