A SIMPLIFIED PROCEDURE FOR THE ISOLATION OF HYPOPHYSEAL GROWTH HORMONE

ABSTRACT A simplified procedure is described for radioimmunoassay of human growth hormone (HGH) in serum using 125I as a label and dextrancharcoal separation of antibody-bound from unbound HGH. Serum HGH levels were measured fasting and after insulin induced hypoglycaemia in four normal subjects, five patients with hypopituitarism and seven with acromegaly. These values agreed well with similar reports by other investigators.

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Decision letter for "Neurochemical phenotype of growth hormone‐responsive cells in the mouse paraventricular nucleus of the hypothalamus"

10.1002/cne.25017/v2/decision1 ◽

2020 ◽

Keyword(s):

Growth Hormone ◽

Paraventricular Nucleus

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Association of pituitary adenoma with neuronal christoma: A TEM study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010016889x ◽

1994 ◽

Vol 52 ◽

pp. 232-233

Author(s):

Eva Horvath ◽

Kalman Kovacs ◽

B. W. Scheithauer ◽

R. V. Lloyd ◽

H. S. Smyth

Keyword(s):

Growth Hormone ◽

Pituitary Adenoma ◽

Pituitary Tumor ◽

Nervous Tissue ◽

Alternative Explanation ◽

Growth Hormone Releasing Hormone ◽

Cell Hyperplasia ◽

Releasing Hormone ◽

Secretory Neurons

The association of a pituitary adenoma with nervous tissue consisting of neuron-like cells and neuropil is a rare abnormality. In the majority of cases, the pituitary tumor is a chromophobic adenoma, accompanied by acromegaly. Histology reveals widely variable proportions of endocrine and nervous tissue in alternating or intermingled patterns. The lesion is perceived as a composite one consisting of two histogenetically distinct parts. It has been suggested that the neuronal component, morphologically similar to secretory neurons of the hypothalamus, may initiate adenoma formation by releasing stimulatory substances. Immunoreactivity for growth hormone releasing hormone (GRH) in the neuronal component of some cases supported this view, whereas other findings such as consistent lack of growth hormone (GH) cell hyperplasia in the lesions called for alternative explanation.Fifteen tumors consisting of a pituitary adenoma and a neuronal component have been collected over a 20 yr. period. Acromegaly was present in 11 patients, was equivocal in one, and absent in 3.

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A rapid silver-impregnation technique on ultra-thin sections for Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100147053 ◽

1993 ◽

Vol 51 ◽

pp. 242-243

Author(s):

K. Chien ◽

R.C. Heusser ◽

M.L. Jones ◽

R.L. Van de Velde

Keyword(s):

Electron Microscopy ◽

Silver Nitrate ◽

Silver Impregnation ◽

Sodium Borate ◽

Renal Diseases ◽

Distilled Water ◽

Thin Sections ◽

Impregnation Technique ◽

Centrifuge Tube ◽

Simplified Procedure

Silver impregnation techniques have been used for the demonstration of the complex carbohydrates in electron microscopy. However, the silver stains were believed to be technically sensitive and time consumming to perform. Currently, due to the need to more specifically evaluate immune complex for localization in certain renal diseases, a simplified procedure in conjunction with the use of the microwave has been developed and applied to renal and other biopsies. The procedure is as follows:Preparation of silver methenamine solution:1. 15ml graduated, clear polystyrene centrifuge tube (Falcon, No. 2099) was rinsed once with distilled water.2. 3% hexamethylene tetramine (methenamine) was added into the centrifuge tube to the 6ml mark.3. 3% silver nitrate was added slowly to the methenamine to the 7ml mark while agitating. (Solution will instantly turn milky in color and then clear rapidly by mixing. No precipitate should be formed).4. 2% sodium borate was added to the solution to the 8ml mark, mixed and centrifuged before use.

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