scholarly journals THE DETERMINATION OF SUGAR IN BLOOD AND IN NORMAL URINE

1926 ◽  
Vol 67 (2) ◽  
pp. 357-370 ◽  
Author(s):  
Otto Folin
Keyword(s):  
Normal Urine

A Note on the Determination and Identification of Cyanide in Biologic Materials

1959 ◽  
Vol 5 (6) ◽  
pp. 587-591 ◽  
Author(s):  
S L Tompsett
Keyword(s):  
Normal Urine

Abstract A procedure is described for the determination of cyanide in biologic fluids. Distinction can be made between simple cyanides, ferrocyanides and ferricyanides, nitroprussides, and cyanogenetic substances, such as amygdalin. Traces of cyanogenetic substances resembling amygdalin could be detected in normal urine. Other types of cyanide-like material could not be detected.

Interference of ofloxacin with determination of urinary porphyrins

1994 ◽  
Vol 40 (3) ◽  
pp. 417-419 ◽  
Author(s):  
N Schoenfeld ◽  
R Mamet
Keyword(s):  
Fluorescence Spectra ◽  
First Generation ◽  
Marked Effect ◽  
Treated Patient ◽  
Fold Increase ◽  
False Positive Diagnosis ◽  
Normal Urine ◽  
Drug Free ◽  
Urinary Porphyrins

Abstract The second-generation quinolone ofloxacin interferes with the screening test of porphyrins. We observed a 20-fold increase in the porphyrin concentration measured in urine of an ofloxacin-treated patient, compared with drug-free normal urine. Two other fluorinated 4-quinolones tested, norfloxacin and ciprofloxacin, had a less marked effect (a twofold increase), whereas the first-generation quinolone, nalidixic acid, did not affect the measured porphyrin concentration at all. The interference is probably due to the overlap in the emission fluorescence spectra of ofloxacin and urinary porphyrins at approximately 600 nm. To avoid a false-positive diagnosis of porphyria, we suggest using HPLC to separate ofloxacin (10-min retention time) from urinary porphyrins (which only start to elute at 12 min). Nonetheless, given a threefold increase in urinary porphyrins observed in the urine of an ofloxacin-treated patient, we also discuss a possible interference of the drug with the metabolism of porphyrins.

Quantitative Determination of Albumin in Normal Urine by an Immunochemical Methoc

1963 ◽  
Vol 15 (3) ◽  
pp. 266-272 ◽  
Author(s):  
R. H. Glass ◽  
C. Risinger ◽  
L. Wide ◽  
C. A. Gemzell
Keyword(s):  
Quantitative Determination ◽  
Normal Urine

A Simple and Rapid Procedure for the Determination of α Amino-nitrogen and Ammonia in Urine

1969 ◽  
Vol 15 (6) ◽  
pp. 433-437 ◽  
Author(s):  
T W Clarkson ◽  
L Ferraio
Keyword(s):  
Urine Sample ◽  
Amino Nitrogen ◽  
Urine Samples ◽  
Rapid Procedure ◽  
Normal Urine ◽  
Colorimetric Procedure

Abstract The ninhydrin-colorimetric procedure for the determination of urinary α amino-nitrogen has been simplified by the use of the Conway microdiffusion unit to remove ammonia from the sample. In this way, urinary ammonia is measured, and at the same time an ammonia-free urine sample is ready for α amino-nitrogen analysis. Values for total and free α amino-nitrogen in normal urine samples were found to be similar to those previously reported by more complicated procedures.

A Biuret Method for Determination of Protein in Normal Urine

1968 ◽  
Vol 14 (12) ◽  
pp. 1160-1171 ◽  
Author(s):  
John Savory ◽  
Pin H Pu ◽  
F William Sunderman
Keyword(s):  
Staining Method ◽  
Close Correlation ◽  
New Drugs ◽  
Day Range ◽  
Quantitative Measurements ◽  
Two Samples ◽  
Amido Black ◽  
Biuret Method ◽  
Normal Urine

Abstract A biuret method has been developed which provides quantitative measurements of protein in normal urine without interference from drugs or pigments. This method is intended for use in monitoring clinical trials of new drugs—to detect nephrotoxicity. Protein is precipitated from duplicate samples of urine by addition of cold ethanolic phosphotungstic acid. The protein precipitates are separated by centrifugation and washed with ethanol. Protein from one of the duplicate samples is dissolvel in biuret reagent. Protein from the second sample is dissolved in an alkaline tartrate reagent which is identical to the biuret reagent, excepting that copper sulfate has been omitted. After 20 min., the differential absorbance of the two samples is measured at 540 mµ. The limit of sensitivity for detection of protein in urine is 0.5 mg./100 ml. The coefficient of variation of replicate analyses of protein in normal urine is 4.2%. The recovery of protein added to urine averages 103 ± 3%. Analyses of urinary protein by the biuret procedure provide close correlation with measurements by an amido black staining method. Systematic search has failed to reveal interference from urinary pigments, compounds, or drugs which are normally or occasionally encountered in hospitalized patients. In 24-hr. collections of urine from 28 healthy adults, the protein concentrations averaged 6.2 mg./100 ml. (range 3.0-12.2), and the protein excretions averaged 77 mg./day (range 40-150).

scholarly journals A METHOD FOR THE DETERMINATION OF SUGAR IN NORMAL URINE

1918 ◽  
Vol 34 (1) ◽  
pp. 195-201
Author(s):  
Stanley R. Benedict ◽  
Emil Osterberg
Keyword(s):  
Normal Urine

Determination of Inorganic Nitrate in Serum and Urine by a Kinetic Cadmium-Reduction Method

1990 ◽  
Vol 36 (8) ◽  
pp. 1440-1443 ◽  
Author(s):  
Najwa K Cortas ◽  
Nabll W Wakid
Keyword(s):  
Reduction Method ◽  
Renal Dialysis ◽  
Time Interval ◽  
Dialysis Patients ◽  
Pseudo First Order Reaction ◽  
First Order ◽  
Inorganic Nitrate ◽  
Normal Urine ◽  
Serum And Urine

Abstract Nitrate in serum and urine was assayed by a modification of the cadmium-reduction method; the nitrite produced was determined by diazotization of sulfanilamide and coupling to naphthylethylene diamine. After samples were deproteinized with Somogyi reagent, the nitrate was reduced by Cu-coated Cd in glycine buffer at pH 9.7 (2.5 to 3 g of Cd granules for a 4-mL reaction mixture). The reduction followed pseudo-first-order reaction kinetics, a convenient time interval for assay being 75 to 90 min. Maximum reduction (85%) occurred at about 2 h. Detection limits in urine or serum were 2 to 250 µmol/L. This method does not require the reaction to go to completion, does not require expensive reagents or equipment, and can assay several samples simultaneously. Repeated assays of two serum pools gave CVs of 9.0% and 4.7% for nitrate concentrations of 31.4 and 80.2 µmol/L, nitrate was 1704.0 ± 1294 (SD) µmol/L (n = 21) in untimed normal urine, 81.8 ± 50.1 µmol/L in serum of 38 renal dialysis patients, and 51.2 ± 26.4 µmol/L in serum of 38 controls.

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