scholarly journals Characterization and epitope mapping of monoclonal antibodies directed against the beta' subunit of the Escherichia coli RNA polymerase.

1992 ◽  
Vol 267 (25) ◽  
pp. 18175-18181
Author(s):  
J Luo ◽  
J.S. Krakow
Keyword(s):  
Escherichia Coli ◽  
Monoclonal Antibodies ◽  
Rna Polymerase ◽  
Epitope Mapping ◽  
Beta Subunit

An amber mutation in the gene encoding the beta' subunit of Escherichia coli RNA polymerase

1982 ◽  
Vol 152 (2) ◽  
pp. 736-746
Author(s):  
S P Ridley ◽  
M P Oeschger
Keyword(s):  
Escherichia Coli ◽  
High Temperature ◽  
Rna Polymerase ◽  
Coli Strain ◽  
Beta Subunit ◽  
Temperature Sensitive ◽  
Amber Mutation ◽  
Gene Encoding ◽  
Amber Suppressor

An Escherichia coli strain carrying an amber mutation (UAG) in rpoC, the gene encoding the beta prime subunit of RNA polymerase, was isolated after mutagenesis with nitrosoguanidine. The mutation was moved into an unmutagenized strain carrying the supD43,74 allele, which encodes a temperature-sensitive su1 amber suppressor, and sue alleles, which enhance the efficiency of the suppressor. In this background, beta prime is not synthesized at high temperature. Suppression of the mutation by the non-temperature-sensitive amber suppressor su1+ yields a protein which is functional at all temperatures examined (30, 37, and 42 degrees C).

scholarly journals Assembly of functional Escherichia coli RNA polymerase containing beta subunit fragments.

1995 ◽  
Vol 92 (10) ◽  
pp. 4591-4595 ◽  
Author(s):  
K. Severinov ◽  
A. Mustaev ◽  
E. Severinova ◽  
I. Bass ◽  
M. Kashlev ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Beta Subunit
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