scholarly journals Cloning and characterization of multiple forms of the human kidney ROM-K potassium channel.

1994 ◽  
Vol 269 (39) ◽  
pp. 24261-24270
Author(s):  
M.E. Shuck ◽  
J.H. Bock ◽  
C.W. Benjamin ◽  
T.D. Tsai ◽  
K.S. Lee ◽  
...  
1997 ◽  
Vol 272 (1) ◽  
pp. 586-593 ◽  
Author(s):  
Mary E. Shuck ◽  
Timothy M. Piser ◽  
Jeffery H. Bock ◽  
Jerry L. Slightom ◽  
Kai S. Lee ◽  
...  

1991 ◽  
Author(s):  
James O. Douglass ◽  
◽  
Macdonald Christie ◽  
John P. Adelman ◽  
R. Alan North
Keyword(s):  

Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 139
Author(s):  
Johanna Detzner ◽  
Elisabeth Krojnewski ◽  
Gottfried Pohlentz ◽  
Daniel Steil ◽  
Hans-Ulrich Humpf ◽  
...  

Human kidney epithelial cells are supposed to be directly involved in the pathogenesis of the hemolytic–uremic syndrome (HUS) caused by Shiga toxin (Stx)-producing enterohemorrhagic Escherichia coli (EHEC). The characterization of the major and minor Stx-binding glycosphingolipids (GSLs) globotriaosylceramide (Gb3Cer) and globotetraosylceramide (Gb4Cer), respectively, of primary human renal cortical epithelial cells (pHRCEpiCs) revealed GSLs with Cer (d18:1, C16:0), Cer (d18:1, C22:0), and Cer (d18:1, C24:1/C24:0) as the dominant lipoforms. Using detergent-resistant membranes (DRMs) and non-DRMs, Gb3Cer and Gb4Cer prevailed in the DRM fractions, suggesting their association with microdomains in the liquid-ordered membrane phase. A preference of Gb3Cer and Gb4Cer endowed with C24:0 fatty acid accompanied by minor monounsaturated C24:1-harboring counterparts was observed in DRMs, whereas the C24:1 fatty acid increased in relation to the saturated equivalents in non-DRMs. A shift of the dominant phospholipid phosphatidylcholine with saturated fatty acids in the DRM to unsaturated species in the non-DRM fractions correlated with the GSL distribution. Cytotoxicity assays gave a moderate susceptibility of pHRCEpiCs to the Stx1a and Stx2a subtypes when compared to highly sensitive Vero-B4 cells. The results indicate that presence of Stx-binding GSLs per se and preferred occurrence in microdomains do not necessarily lead to a high cellular susceptibility towards Stx.


Author(s):  
Alessandra Balduini ◽  
Cristiano Fava ◽  
Christian A. Di Buduo ◽  
Vittorio Abbonante ◽  
Alessandra Meneguzzi ◽  
...  

1980 ◽  
Vol 623 (2) ◽  
pp. 317-328 ◽  
Author(s):  
Giuseppina Conio ◽  
Pietro Ghiani ◽  
Eligio Patrone ◽  
Vincenzo Trefiletti ◽  
Biancamaria Uva ◽  
...  

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