A high-throughput method to quantify shape differences for three-dimensional imaging technologies

2006 ◽  
Vol 39 ◽  
pp. S651
Author(s):  
E. Kristensen ◽  
T.E. Parsons ◽  
B. Hallgrimsson ◽  
S. Boyd
Keyword(s):  
High Throughput ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Imaging Technologies ◽  
High Throughput Method

scholarly journals Diel Growth Cycle of Isolated Leaf Discs Analyzed with a Novel, High-Throughput Three-Dimensional Imaging Method Is Identical to That of Intact Leaves

2009 ◽  
Vol 149 (3) ◽  
pp. 1452-1461 ◽  
Author(s):  
Bernhard Biskup ◽  
Hanno Scharr ◽  
Andreas Fischbach ◽  
Anika Wiese-Klinkenberg ◽  
Ulrich Schurr ◽  
...  
Keyword(s):  
High Throughput ◽  
Three Dimensional ◽  
Growth Cycle ◽  
Imaging Method ◽  
Three Dimensional Imaging ◽  
Leaf Discs ◽  
Intact Leaves

scholarly journals Cameraless high-throughput three-dimensional imaging flow cytometry

Optica ◽  
2019 ◽  
Vol 6 (10) ◽  
pp. 1297 ◽  
Author(s):  
Yuanyuan Han ◽  
Rui Tang ◽  
Yi Gu ◽  
Alex Ce Zhang ◽  
Wei Cai ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Imaging Flow Cytometry

High throughput method for prototyping three-dimensional, paper-based microfluidic devices

Lab on a Chip ◽  
2012 ◽  
Vol 12 (15) ◽  
pp. 2630 ◽  
Author(s):  
Gregory G. Lewis ◽  
Matthew J. DiTucci ◽  
Matthew S. Baker ◽  
Scott T. Phillips
Keyword(s):  
High Throughput ◽  
Three Dimensional ◽  
Microfluidic Devices ◽  
High Throughput Method

scholarly journals Rapid Optical Clearing for High-Throughput Analysis of Tumour Spheroids

2021 ◽  
Author(s):  
Gency Gunasingh ◽  
Alexander Browning ◽  
Nikolas Haass
Keyword(s):  
High Throughput ◽  
Three Dimensional ◽  
Quality Data ◽  
High Quality ◽  
Size Distortion ◽  
High Throughput Analysis ◽  
High Quality Data ◽  
Tumour Spheroids ◽  
High Throughput Method ◽  
Significant Size

Tumour spheroids are fast becoming commonplace in basic cancer research and drug development. Obtaining high-quality data relating to the inner structure of spheroids is important for analysis, yet existing techniques often use equipment that is not commonly available, are expensive, laborious, cause significant size distortion, or are limited to relatively small spheroids. We present a high-throughput method of mounting, clearing, and imaging tumour spheroids that causes minimal size distortion. Spheroids are mounted in an agarose gel to prevent movement, cleared using a solution prepared from commonly available materials, and imaged using confocal microscopy. We find that our method yields high quality two- and three-dimensional images that provide information about the inner structure of spheroids.

scholarly journals New views on three-dimensional imaging technologies for glaucoma

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Maria A. Guzman Aparicio ◽  
Teresa C. Chen
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Imaging Technologies

scholarly journals Automated High-Throughput Light-Sheet Fluorescence Microscopy of Larval Zebrafish

2018 ◽  
Author(s):  
Savannah L. Logan ◽  
Christopher Dudley ◽  
Ryan P. Baker ◽  
Michael J. Taormina ◽  
Edouard A. Hay ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
High Throughput ◽  
Three Dimensional ◽  
Light Sheet ◽  
Individual Variability ◽  
Three Dimensional Imaging ◽  
Larval Zebrafish ◽  
Neutrophil Number ◽  
Spatially Resolved ◽  
Light Sheet Fluorescence Microscopy

ABSTRACTLight sheet fluorescence microscopy enables fast, minimally phototoxic, three-dimensional imaging of live specimens, but is currently limited by low throughput and tedious sample preparation. Here, we describe an automated high-throughput light sheet fluorescence microscope in which specimens are positioned by and imaged within a fluidic system integrated with the sheet excitation and detection optics. We demonstrate the ability of the instrument to rapidly examine live specimens with minimal manual intervention by imaging fluorescent neutrophils over a nearly 0.3 mm3 volume in dozens of larval zebrafish. In addition to revealing considerable inter-individual variability in neutrophil number, known previously from labor-intensive methods, three-dimensional imaging allows assessment of the correlation between the bulk measure of total cellular fluorescence and the spatially resolved measure of actual neutrophil number per animal. We suggest that our simple experimental design should considerably expand the scope and impact of light sheet imaging in the life sciences.

Three-dimensional imaging technologies: a priority for the advancement of tissue engineering and a challenge for the imaging community

2016 ◽  
Vol 10 (1) ◽  
pp. 24-45 ◽  
Author(s):  
Laura Teodori ◽  
Annunziata Crupi ◽  
Alessandra Costa ◽  
Alberto Diaspro ◽  
Susanne Melzer ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Imaging Technologies ◽  
A Priority
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