Internal jugular phlebectasia communicating with a cervicomediastinal lipoma: a case report

Venous aneurysms are an atypical presentation of neck masses in the paediatric population. The evaluation and surgical removal of internal jugular vein phlebectasia and a lipoma coexisting are described in this report. Internal jugular vein phlebectasia is theorised as a congenital defect and is becoming more common with advancing imaging technologies. Both phlebectasia and lipomas are considered benign conditions, but clinicians must be aware of tumours producing mass effect.

A framework for multiplex imaging optimization and reproducible analysis

Multiplex imaging technologies are increasingly used for single-cell phenotyping and spatial characterization of tissues; however, transparent methods are needed for comparing the performance of platforms, protocols and analytical pipelines. We developed a python software, jinxif, for reproducible image processing and utilize Jupyter notebooks to share our optimization of signal removal, antibody specificity, background correction and batch normalization of the multiplex imaging with a focus on cyclic immunofluorescence (CyCIF). Our work both improves the CyCIF methodology and provides a framework for multiplexed image analytics that can be easily shared and reproduced.

The Promise of Hyperspectral Imaging for the Early Detection of Crown Rot in Wheat

Crown rot disease is caused by Fusarium pseudograminearum and is one of the major stubble-soil fungal diseases threatening the cereal industry globally. It causes failure of grain establishment, which brings significant yield loss. Screening crops affected by crown rot is one of the key tools to manage crown rot, because it is necessary to understand disease infection conditions, identify the severity of infection, and discover potential resistant varieties. However, screening crown rot is challenging as there are no clear visible symptoms on leaves at early growth stages. Hyperspectral imaging (HSI) technologies have been successfully used to better understand plant health and disease incidence, including light absorption rate, water and nutrient distribution, and disease classification. This suggests HSI imaging technologies may be used to detect crown rot at early growing stages, however, related studies are limited. This paper briefly describes the symptoms of crown rot disease and traditional screening methods with their limitations. It, then, reviews state-of-art imaging technologies for disease detection, from color imaging to hyperspectral imaging. In particular, this paper highlights the suitability of hyperspectral-based screening methods for crown rot disease. A hypothesis is presented that HSI can detect crown-rot-infected plants before clearly visible symptoms on leaves by sensing the changes of photosynthesis, water, and nutrients contents of plants. In addition, it describes our initial experiment to support the hypothesis and further research directions are described.

An end-to-end workflow for multiplexed image processing and analysis

Simultaneous profiling of the spatial distributions of multiple biological molecules at single-cell resolution has recently been enabled by the development of highly multiplexed imaging technologies. Extracting and analyzing biologically relevant information contained in complex imaging data requires the use of a diverse set of computational tools and algorithms. Here, we report the development of a user-friendly, customizable, and interoperable workflow for processing and analyzing data generated by highly multiplexed imaging technologies. The steinbock framework supports image pre-processing, segmentation, feature extraction, and standardized data export. Each step is performed in a reproducible fashion. The imcRtools R/Bioconductor package forms the bridge between image processing and single-cell analysis by directly importing data generated by steinbock. The package further supports spatial data analysis and integrates with tools developed within the Bioconductor project. Together, the tools described in this workflow facilitate analyses of multiplexed imaging raw data at the single-cell and spatial level.