Retention parameters and eluent composition in thin—layer and high—performance liquid chromatography of aromatic nitro compounds in reversed—phase systems

1981 ◽  
Vol 205 (2) ◽  
pp. 451-456 ◽  
Author(s):  
Marie Beiganowska ◽  
Edward Soczewiński
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
High Performance ◽  
Nitro Compounds ◽  
Reversed Phase ◽  
Aromatic Nitro Compounds ◽  
Phase Systems ◽  
Aromatic Nitro ◽  
Retention Parameters

Chromatographic behaviour of some porphyrins and their complexes with Zn, Pd(II) and Cu(II)

2000 ◽  
Vol 04 (02) ◽  
pp. 209-214 ◽  
Author(s):  
M. I. UVAROVA ◽  
G. D. BRYKINA ◽  
O. A. SHPIGUN
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Zinc Complexes ◽  
Acetate Concentration ◽  
Hydrophobic Compounds ◽  
Retention Parameters

In this work the influence of the porphyrin structure and of the nature of the mobile phase upon retention parameters is examined by means of reversed phase high-performance liquid chromatography (HPLC). Acetonitrile–ethyl acetate and other mixtures were used as eluents. An increase in the retention of azo- and benzosubstituted porphyrins as well as of those containing a large number of carbon atoms as substituents of macrocycles may be noted. A variation in the polarity of the mobile phase affects the retention of the ligands more than that of their zinc complexes. The retention of the most hydrophobic compounds may be well described in coordinates lg k'– lg M. For less hydrophobic porphyrins these dependences are close to linear only within limited intervals of mobile phase ethyl acetate concentration. The best separation of zinc complexes was achieved with acetonitrile as the eluent. The detection limit of porphyrin ligands and complexes with metals is n × 10-8 M.

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