Two-dimensional thin-layer chromatography of Dns-amino acids on reversed-phase silica gel

1980 ◽  
Vol 193 (3) ◽  
pp. 421-426 ◽  
Author(s):  
K. Macek ◽  
Z. Deyl ◽  
M. Smrž
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Reversed Phase ◽  
Two Dimensional ◽  
Layer Chromatography

scholarly journals Normal and reversed phase thin-layer chromatography of new 16, 17-secoestrone derivatives

2003 ◽  
Vol 68 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Marijana Acanski ◽  
Suzana Jovanovic-Santa ◽  
Lidija Jevric
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Reversed Phase ◽  
Retention Behavior ◽  
Mobile Phases ◽  
Layer Chromatography

The retention behavior and separation ability of a series of new 16,17-secoestrone derivatives has been studied on silica gel, alumina and C-18 silica gel layers with non-aqueous and aqueous-organic mobile phases. The retention behavior and separation ability are discussed in terms of the nature of the solute, eluent and stationary phase.

Identification and Quantitation of Panthenol and Pantothenic Acid in Pharmaceutical Preparations by Thin-Layer Chromatography and Densitometry

1992 ◽  
Vol 75 (5) ◽  
pp. 898-901 ◽  
Author(s):  
Saurendra S Nag ◽  
Saroj K Das
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Benzyl Alcohol ◽  
Silica Gel ◽  
Pantothenic Acid ◽  
Pharmaceutical Preparations ◽  
Ninhydrin Reaction ◽  
Layer Chromatography

Abstract A rapid, simple, and specific thin-layer chromatographic (TLC) method has been developed for the estimation of panthenol and pantothenic acid in pharmaceutical preparations containing other vitamins, amino acids, syrups, enzymes, etc. The vitamin is extracted with ethanol (tablets and capsules) or benzyl alcohol (liquid oral preparations) and isolated from other ingredients by TLC on silica gel with isopropanol-water (85 + 15, v/v) as developing solvent. β-Alanine (pantothenate) or β-alanol (panthenol) is liberated by heating for 20 min at 160°C. The liberated amines are visualized by ninhydrin reaction and estimated by spectrodensitometry at 490 nm. Recoveries for panthenol and pantothenic acid were 99.8 ± 2.25% and 100.2 ± 1.7%, respectively.

Carbonyl Compounds in Salted Cod.: I. Thin-Layer Chromatography of Aliphatic Monocarbonyl 2,4-Dinitrophenylhydrazones

1965 ◽  
Vol 22 (1) ◽  
pp. 17-25 ◽  
Author(s):  
M. Yurkowski
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Propylene Glycol ◽  
Silica Gel ◽  
Carbonyl Compounds ◽  
Two Dimensional ◽  
Layer Chromatography

Methods are described for the separation of 2,4-dinitrophenylhydrazones of normal aliphatic monocarbonyl compounds by thin-layer chromatography. Magnesia-cellulose thin-layer plates are used to separate mixtures of these compounds into classes (n-alkanals, n-alkan-2-ones, n-alk-2-enals, and n-alka-2,4-dienal). Propylene glycol- and petrolatum-impregnated silica gel plates were also used to separate each class into homologues. The former impregnant resolved the compounds with less than seven carbons and the latter with more than six carbons. Two-dimensional separation was accomplished by resolving a mixture of these compounds into classes on magnesia-cellulose in the first dimension and the classes into homologues on either petrolatumor propylene glycol-impregnated silica gel plates in the second dimension.

Two-Dimensional Thin-Layer Chromatography on Two-Layer Plates of Amino Acids

1970 ◽  
Vol 16 (8) ◽  
pp. 662-666 ◽  
Author(s):  
F Kraffczyk ◽  
R Helger ◽  
H Lang
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Two Dimensions ◽  
Two Dimensional ◽  
One Dimensional ◽  
Acid Cation ◽  
Solvent Systems ◽  
Strong Acids ◽  
Layer Chromatography

Abstract Separation of the amino acids in urine by use of thin-layer chromatography (TLC) has hitherto required that the specimen be first desalted and then chromatographed in two dimensions with at least two pairs of developing solvent systems. We wished to simplify both steps. The customary method of desalting on a column is replaced by desalting on a plate that supports a strongly acid cation-exchanger and a cellulose layer. This method, originally developed for one-dimensional TLC, is used here for two-dimensional TLC. Urine is applied to the ion-exchange layer and strong acids and neutral substances are removed with water. The amino acids are then chromatographed into the cellulose layer, and are separated there two dimensionally with a newly devised pair of developing solutions. This pair of solvents separates nearly all of the amino acids in urine.

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