Optimization of eluent composition and column length in liquid chromatography

1986 ◽  
Vol 353 ◽  
pp. 139-152 ◽  
Author(s):  
Vratislav Svoboda ◽  
Jana Voc̆ková
Keyword(s):  
Liquid Chromatography ◽  
Column Length ◽  
Eluent Composition

Preparation of short glass microbore columns for liquid chromatography and their properties

1984 ◽  
Vol 49 (4) ◽  
pp. 764-771 ◽  
Author(s):  
Danuše Kouřilová ◽  
Karel Šlais ◽  
Miloš Krejčí
Keyword(s):  
Liquid Chromatography ◽  
Mobile Phase ◽  
Linear Part ◽  
Theoretical Plate ◽  
Outer Diameter ◽  
Column Length ◽  
Microbore Columns ◽  
Inner Diameter ◽  
Glass Columns ◽  
Short Glass

The preparation is described of thick-walled glass columns, inner diameter 0.5 mm and outer diameter 4.5 mm, packed with Separon SI C 18 (dp = 6 and 10 μm) from suspension under pressures of 20-35 MPa in the presence of an ionogenic detergent; the column length ranged from 5 to 30 cm. The minimum value of the height equivalent to a theoretical plate was Hmin = 2.8 - 3.3dp. The effect of the extra-column contributions to the dispersion on the slope of the linear part of the dependence of the height equivalent to a theoretical plate on the mobile phase velocity was examined and found to become significant at higher velocities, thereby imposing limitations on the shortening of the time of analysis.

Effects of column length, particle size, gradient length and flow rate on peak capacity of nano-scale liquid chromatography for peptide separations

2007 ◽  
Vol 1147 (1) ◽  
pp. 30-36 ◽  
Author(s):  
Hongji Liu ◽  
Jeffrey W. Finch ◽  
Michael J. Lavallee ◽  
Robert A. Collamati ◽  
Christopher C. Benevides ◽  
...  
Keyword(s):  
Particle Size ◽  
Liquid Chromatography ◽  
Flow Rate ◽  
Peak Capacity ◽  
Column Length ◽  
Nano Scale ◽  
Peptide Separations ◽  
Size Gradient
Sign in / Sign up

Export Citation Format

Share Document