Preparation of short glass microbore columns for liquid chromatography and their properties
1984 ◽
Vol 49
(4)
◽
pp. 764-771
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Keyword(s):
The preparation is described of thick-walled glass columns, inner diameter 0.5 mm and outer diameter 4.5 mm, packed with Separon SI C 18 (dp = 6 and 10 μm) from suspension under pressures of 20-35 MPa in the presence of an ionogenic detergent; the column length ranged from 5 to 30 cm. The minimum value of the height equivalent to a theoretical plate was Hmin = 2.8 - 3.3dp. The effect of the extra-column contributions to the dispersion on the slope of the linear part of the dependence of the height equivalent to a theoretical plate on the mobile phase velocity was examined and found to become significant at higher velocities, thereby imposing limitations on the shortening of the time of analysis.
2006 ◽
Vol 135
(3)
◽
pp. 209-218
◽
2020 ◽
Vol 43
(22)
◽
pp. 4094-4102
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1996 ◽
Vol 61
(s1)
◽
pp. 161-163
◽
1994 ◽
Vol 59
(3)
◽
pp. 569-574
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2017 ◽
Vol 1484
◽
pp. 34-40
◽
1977 ◽
Vol 23
(12)
◽
pp. 2288-2291
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Keyword(s):