High-performance gel permeation chromatography of bovine eye lens proteins in combination with low-angle laser light scattering

1982 ◽  
Vol 252 ◽  
pp. 255-267 ◽  
Author(s):  
Jacques G. Bindels ◽  
Ben M. De Man ◽  
Herman J. Hoenders
Keyword(s):  
Light Scattering ◽  
High Performance ◽  
Laser Light ◽  
Gel Permeation Chromatography ◽  
Laser Light Scattering ◽  
Eye Lens ◽  
Lens Proteins ◽  
Gel Permeation ◽  
Eye Lens Proteins

Detection of serum proteins by high-pressure gel-permeation chromatography with low-angle laser light scattering, compared with analytical ultracentrifugation.

1986 ◽  
Vol 32 (2) ◽  
pp. 363-367 ◽  
Author(s):  
W Flapper ◽  
P J van den Oetelaar ◽  
C P Breed ◽  
J Steenbergen ◽  
H J Hoenders
Keyword(s):  
High Pressure ◽  
Light Scattering ◽  
Analytical Ultracentrifugation ◽  
Laser Light ◽  
Serum Proteins ◽  
Gel Permeation Chromatography ◽  
Laser Light Scattering ◽  
Gel Permeation ◽  
Human Sera ◽  
Molecular Masses

Abstract Human sera were subjected to analytical ultracentrifugation and "high-pressure" gel-permeation chromatography on a system of combined TSK Gel G5000 PW and G3000 SW columns. The chromatographic method produced remarkably superior resolution of the proteins, especially those exceeding 100 000 Da. We calculated the molecular masses of eluted fractions on the basis of their detection by low-angle laser light scattering and their differential refractive index. We discuss the results in relation to the clinical data.

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