Liquid chromatographic determination of cyadox in medicated feeds and in the contents of the porcine gastrointestinal tract with fluorescence detection

Abstract A rapid, reliable separation and quantitation of zoalene (3,5-dinitroo-toluamide) from feeds is accomplished by using reverse phase liquid chromatography (LC) and ultraviolet detection. An extraction technique which is similar to the present AOAC official colorimetric method is used before chromatographic analysis. This extraction is followed by an activated alumina cleanup and LC to separate zoalene from feed matrix. The methodology was applied to a variety of spiked feed matrices, and yielded good recoveries. Liquid chromatographic results were shown to correlate with colorimetric determinations.

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High-performance liquid chromatographic determination of 1,2,3,4-tetrahydroisoquinoline in rat brain with fluorescence detection

Analytical Biochemistry ◽

10.1016/0003-2697(91)90313-i ◽

1991 ◽

Vol 195 (1) ◽

pp. 168-171 ◽

Cited By ~ 9

Author(s):

Junichi Ishida ◽

Masatoshi Yamaguchi ◽

Masaru Nakamura

Keyword(s):

Rat Brain ◽

Fluorescence Detection ◽

High Performance ◽

Chromatographic Determination ◽

High Performance Liquid Chromatographic ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

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Liquid Chromatographic Determination of Ivermectin in Feed

Journal of AOAC International ◽

10.1093/jaoac/81.4.869 ◽

1998 ◽

Vol 81 (4) ◽

pp. 869-872 ◽

Cited By ~ 4

Author(s):

Stephen J Doherty ◽

Allen Fox ◽

David W Fink

Keyword(s):

Solid Phase ◽

Chromatographic Determination ◽

Phase Extraction ◽

Coefficients Of Variation ◽

Significant Difference ◽

Liquid Chromatographic Determination ◽

Chromatographic Measurement ◽

Liquid Chromatographic ◽

Medicated Feeds

Abstract An analytical method for determining ivermectin in feed at 0.50- 3 ppm is presented. The method is based on liquid chromatographic measurement after sample preparation by adsorption chromatography on alumina and solid-phase extraction. Two complete, final, finished medicated feeds and the corresponding control feeds used in their preparation were analyzed. Recoveries from feeds fortified at 50-150% of the 2 ppm ivermectin use concentration also were determined. Mean recoveries from replicate analyses ranged from 90 to 100%, and coefficients of variation (CVs) were less than 4.5%. No significant interferences were found in control feeds. The pooled distribution of individual analytical results (n = 100) gave a mean recovery of 100%, a recovery range of 90-111%, and an overall CV of 5.5%. Resolution of the total variance into its 2 components gave a withinlaboratory CV of 4.1% and a between-laboratory CV of 3.4%. There was no significant difference in recoveries among laboratories, days, concentrations, and feed base or between fortified and medicated feeds (P > 0.2)

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Liquid Chromatographic Determination of Zearalenone and Zearalenol in Animal Feeds and Grains, Using Fluorescence Detection

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/69.5.894 ◽

1986 ◽

Vol 69 (5) ◽

pp. 894-898 ◽

Cited By ~ 3

Author(s):

Renee W Bagneris ◽

Jean A Gaul ◽

George M Ware

Keyword(s):

Fluorescence Detection ◽

Limit Of Detection ◽

Chromatographic Determination ◽

Excitation Wavelength ◽

Animal Feeds ◽

Coefficients Of Variation ◽

Liquid Chromatographic Determination ◽

Sorghum Silage ◽

Liquid Chromatographic

Abstract A liquid chromatographic (LC) method was developed for the determination of zearalenone and zearalenol in grains and mixed animal feeds. Samples are extracted with chloroform and purified by a baseacid liquid-liquid partition. Zearalenone and zearalenol are separated by reverse phase LC and determined by fluorescence detection, excitation wavelength 236 nm with a 418 nm cutoff filter. The method was applied to the determination of zearalenone and zearalenol in 395 survey samples of corn, oats, barley, sorghum, silage, and finished feeds. The limit of detection is 10 ng/g for both toxins. The range of naturally occurring toxins found was 10-4000 ng/g. Average recoveries were 84% for zearalenone and 69% for zearalenol. Coefficients of variation were 24.6% for zearalenone and 30.8% for zearalenol for crop year 1980, and 28.3% for zearalenone and 22.0% for zearalenol for crop year 1981.

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