Pesticide Residue Analysis from Winter Vegetables Collected from Six Markets of Rajshahi Bangladesh

Pesticide residues in vegetables have become a major concern associated with food safety issues. Five types of winter vegetables like bean, brinjal, tomato, cauliflower and cabbage were collected from six markets of Rajshahi District during January, , 2019 in Bangladesh. Residues of commonly used pesticides (Acephate, Dimethoate, Diazinon, Fenotrothion, Malathion, Chlorpyrifos and Quinalphos) in winter vegetable samples were determined using Gas Chromatography-Flame Thermionic Detector (GC-FTD) procedures. A quick, easy, cheap, effective, rugged and safe (QuEChERS) method with acetate buffering (AOAC Official Method 2007.01) was used for sample preparation. Out of 30 samples, pesticide residues were found only in one brinjal and two tomato samples. The result showed that tomato samples collected from Shaheb bazar and Kharkhari bazar of Rajshahi City contained 0.047 mg/kg and 0.139 mg/kg dimethoate residue, respectively. Brinjal sample collected from Shalbagan bazar, Rajshahi, contained 0.052 mg/kg dimethoate residues. The detected pesticide residue dimethoate of these three samples was higher than maximum residue level (0.01 mg/kg). Environ. Sci. & Natural Resources, 12(1&2): 43-50, 2019

APLICACIÓN DE BACTERIAS ÁCIDO-LÁCTICAS PROVENIENTES DEL MUCILAGO DE CACAO COMO AGENTE DE CONSERVACIÓN DE LA PAPAYA

El objetivo de esta investigación fue evaluar la conservación de la papaya con aplicación de bacterias ácidos lácticas provenientes del mucilago de cacao, se utilizaron diferentes porcentajes de aplicación (0, 5, 10 %) por aspersión a temperatura ambiente 35°C, se valoró el cambio en las características físicas, químicas, y microbiológicas en un lapso de 14 días, el peso de la papaya fluctuó entre 1 – 1.4 kg, considerando una madurez de cosecha. Se realizó un Diseño Completamente al Azar (DCA) con arreglo bifactorial AxB, para la comparación de medias de los tratamientos a estudiar se utilizó la prueba de rangos de Tukey al 5%. Las variables evaluadas (pH, pérdida de peso, acidez) dio como resultado que el tratamiento T9 (10% BAL; 14 Días) fue el que mantuvo mejores propiedades de almacenamiento transcurrido el tiempo de 14 días de conservación, el color de la fruta se mantuvo en niveles adecuados. Palabras Clave: características sensoriales, frutas frescas, procesamiento de frutas, tiempo de vida útil. Referencias [1]Organización de las Naciones Unidas para la Agricultura y la Alimentación FAO, “Procesamiento de frutas y verduras,” Roma, 2014. [2]E. Rodríguez-Sauceda, “Uso de agentes antimicrobianos naturales en la conservación de frutas y hortalizas,” Ra Ximhai, vol. 7, pp. 153–170, 2011, doi: 10.35197/rx.07.01.2011.14.er. [3]R. Raybaudi-Massilia, R. Soliva, y O. Martín, “Simposio Iberoamericano de Hortalizas Frescas , 1 / Congreso Nacional de Procesamiento Mínimo de Frutas y Hortalizas , 4 ( 2006 ),” 2006. [4]M. Valle, “Aplicación de recubrimientos comestibles para mantener la calidad de frutillas congeladas,” p. 211, 2012. [5]A. Garcia Figueroa, A. Ayala-Aponte, y M. I. Sánchez-Tamayo, “Efecto de recubrimientos comestibles de Aloe vera y alginato de sodio sobre la calidad poscosecha de fresa,” Rev. U.D.C.A Actual. Divulg. Científica, vol. 22, no. 2, 2019, doi: 10.31910/rudca.v22.n2.2019.1320. [6]K. Córdova y A. Loor, “Prolongación de la vida útil de la papaya ( Carica papaya ) en percha por inmersión en soluciones de propóleo en etanol,” 2014. [7]I. M. Brasil, C. Gomes, A. Puerta-Gomez, M. E. Castell-Perez, y R. G. Moreira, “Polysaccharide-based multilayered antimicrobial edible coating enhances quality of fresh-cut papaya,” LWT - Food Sci. Technol., vol. 47, no. 1, pp. 39–45, 2012, doi: 10.1016/j.lwt.2012.01.005. [8]Z. Kalvatchev, D. Garzaro, y F. Guerra Cedezo, “Theobroma cacao L.: Un nuevo enfoque para nutrición y salud,” Rev. Agroaliment., vol. 4, no. 6, pp. 23–25,1998. [9]S. Vasquez, H. Suárez, y S. Zapata, “Utilización de sustancias antimicrobianas producidas por bacterias acido lácticas en la conservación de la carne,” Rev. Chil. Nutr., vol. 36, no. 1, pp. 64–71, 2009, doi: 10.1097/PHH.0000000000000678. [10]R. Rivera-Rebollar, S. Cabrera-Calderón, A. Lira-Vargas, M. Trejo-Márquez, y S. Pascual-Bustamante, “Efecto de recubrimiento de carboximetilcelulosaadicionado con extracto de epazote en el control de hongos postcosecha de papaya, jitomate y chile,” Investig. y Desarro. en Cienc. y Tecnol. Aliment., vol.1, no. 2, pp. 379–384, 2016. [11]A. Sañudo, J. Siller, T. Osuna, D. Muy, G. López, y J. Labavitch, “Control de la maduración en frutos de papaya (Carica papaya L.) con 1-metilciclopropenoy ácido 2- cloroetil fosfónico,” Rev. Fitotec. Mex., vol. 31, no. 2, pp. 141–147, 2008. [12]A. Almeida-Castro, J. Reis-Pimentel, D. Santos-Souza, y T. Vieira, “Estudio de la conservación de la papaya (Carica papaya L.) asociado a la aplicación de películas comestibles,” Rev. Venez. Cienc. y Tecnol. Aliment., vol. 2, no. 1, pp. 49–060, 2011. [13]A. Jimenes-Trujillo, “Recubrimiento Comestible a Base de Aloe Vera (Aloe barbadensis miller) para Papaya (Carica papaya) Y Guayaba (Psidium guajava)Como Alternativa de Alimentos de IV Gama,” Universidad Técnica del Norte, 2017. [14]Instituto Ecuatoriano de Normalización, “NTE INEN-ISO 750:2013 Productos Vegetales y de Frutas - Determinación de la Acidez Titulable (IDT),” 2013. [15]Instituto Ecuatoriano de Normalización., “NTE INEN 1756. Frutas Frescas. Papaya. Requisitos,” Ecuador, 1990. [16]A. International, “AOAC: Official Methods of Analysis.”. [17]Instituto Ecuatoriano de Normalización, “Norma Técnica Ecuatoriana NTE INEN-ISO 2173:2013,” 2013. [18]A. Miranda, A. Alvis, y G. Arrazola, “Efectos de dos recubrimientos sobre la calidad de la papaya (Carica papaya) variedad tainung,” Temas Agrar., vol. 19,no. 1, pp. 7–18, 2014. [19]L. Konda et al., “InfluêncIa da atmosfera modIfIcada por fIlmes plástIcos sobre a qualIdade do mamão armazenado sob refrIgeração 1,” 2006. [20]A. Castricini, “Aplicação de Revestimentos Comestíveis para Conservação de Mamões (Carica papaya L.) ‘Golden,’” UNIVERSIDADE FEDERAL RURALDO RIO DE JANEIRO, 2009. [21]M. Mata, M. del C. Vásquez, N. Higinio, y D.Hernandéz, “Estudio comparativo de bio-recubrimientos a partir de Manihot esculenta y Phaseolus vulgarisempleadas como recubrimiento en uvas moradas,” Rev. Ciencias Ambient. y Recur. Nat., vol. 2, no. 5, pp. 11–25, 2016. [22]M. Maskan, “Microwave/air and microwave finish drying of banana,” J. Food Eng., vol. 44, no. 2, pp. 71–78, 2000, doi: 10.1016/S0260-8774(99)00167-3. [23]R. Torres, E. Montes, O. Pérez, y R. Andrade,“Relación del color y del estado de madurez con las propiedades fisicoquímicas de frutas tropicales,” Inf. Tecnológica, vol. 24, no. 3, pp. 51–56, 2013, doi: 10.4067/S0718-07642013000300007.

Natural Occurrence of Ochratoxin A in Blood and Milk Samples from Jennies and Their Foals after Delivery

An assessment of the natural ochratoxin A (OTA) exposure of seven Martina Franca jennies was carried out by analyzing blood and milk samples collected close to and after delivery. A total of 41 and 34 blood samples were collected from jennies and foals, respectively, and analyzed by ELISA. A total of 33 milk samples were collected from jennies and analyzed by the HPLC/FLD method based on IAC clean-up. Furthermore, 53 feed samples were collected from January to September and analyzed by a reference method (AOAC Official Method No. 2000.03) for OTA content. Feed samples showed OTA levels up to 2.7 ng/g with an incidence of 32%, while the OTA incidence rate in jennies’ blood samples was 73%, with a median value of 97 ng/L and concentrations ranging from <LOD to 6000 ng/L. A seasonal effect on OTA levels in positive blood samples was observed, with increases in the 53% of the positive ones from April to June. Concerning foals, the incidence rate of blood samples was 50%, with a median value of 52 ng/L, and concentrations ranged from <LOD to 4034 ng/L. The incidence of milk samples was 36%, with levels ranging from <LOD to 82 ng/L. In conclusion, the results showed a natural exposure of jennies and foals to OTA, and its presence in jenny milk could pose a risk for human newborns, considering its well-known nutritional and health properties.

Caracterización nutricional y funcional de la harina de mashua

La presente investigación propone una caracterización de la harina de mashua (Tropaeolum tuberosum) desde el punto de vista nutricional y funcional para establecer la utilidad de su aplicación. Se inició con la obtención de la harina luego de un proceso de selección, limpieza, lavado y troceado, secado, molienda y tamizado se procedió a analizar las muestras tomando en cuenta los métodos establecidos por las Normas Técnicas Ecuatorianas INEN y la AOAC (Official Methods of Analysis of the Association). El análisis proximal realizado a las muestras de harina de mashua reflejan un contenido de humedad promedio de 10,46±0,09%, el aporte de proteína se encuentra en un rango de 12,34±0,08%, mientras que el contenido de cenizas presenta un valor promedio de 4,66±0,10%. En cuanto al contenido de grasa se obtuvo un resultado promedio de 0,81±0,24% y con respecto a la fibra un promedio de 7,07±0,009%. Para carbohidratos el resultado promedio fue de 64,67±0,14%. Del análisis funcional de la harina de mashua se obtiene un valor de pH igual a 5,5±0,02 y un porcentaje de acidez de 1,08 ±0,025%, además la capacidad de retención de agua CRA mostró un porcentaje que varía entre 5,05 y 8,63% para un rango de temperaturas de 50 a 80°C, demostrándose con este resultado que la temperatura incide significativamente en la CRA. Las muestras de harina de mashua analizadas cumplen con los parámetros establecidos y son de calidad físico-química y funcional aceptable.

Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions

Different batches of biomass/feed quality maize contaminated by aflatoxins were processed at the industrial scale (a continuous process and separate discontinuous steps) to evaluate the effect of different cleaning solutions on toxin reduction. The investigated cleaning solutions included: (i) mechanical size separation of coarse, small and broken kernels, (ii) removal of dust/fine particles through an aspiration channel, (iii) separation of kernels based on gravity and (iv) optical sorting of spatial and spectral kernel defects. Depending on the sampled fraction, dynamic or static sampling was performed according to the Commission Regulation No. 401/2006 along the entire cleaning process lines. Aflatoxin analyses of the water–slurry aggregate samples were performed according to the AOAC Official Method No. 2005.008 based on high-performance liquid chromatography and immunoaffinity column cleanup of the extracts. A significant reduction in aflatoxin content in the cleaned products, ranging from 65% to 84% with respect to the uncleaned products, was observed when continuous cleaning lines were used. Additionally, an overall aflatoxin reduction from 55% to 94% was obtained by combining results from separate cleaning steps. High levels of aflatoxins (up to 490 µg/kg) were found in the rejected fractions, with the highest levels in dust and in the rejected fractions from the aspirator and optical sorting. This study shows that a cleaning line combining both mechanical and optical sorting technologies provides an efficient solution for reducing aflatoxin contamination in maize.

Determination of Lutein, β-Carotene, and Lycopene in Infant Formula and Adult Nutritionals by Ultra-High Performance Liquid Chromatography: Collaborative Study, Final Action 2016.13 for β-Carotene and Lycopene Only

Background Lutein, β-carotene, and lycopene are among the most common carotenoids present in human milk and are frequently added to infant formula and adult nutritionals. Objective A collaborative study was conducted to assess the interlaboratory performance of AOAC Official MethodSM2016.13 for the determination of lutein, β-carotene, and lycopene in infant formula and adult nutritionals. Methods Thirteen laboratories agreed to participate in the study and 10 laboratories from seven different countries reported results. The study samples included blind duplicates of 6 matrices fortified with lutein, 7 matrices fortified with β-carotene, and 1 fortified with lycopene. NIST SRM 1869 was included in the sample set as a reference material. Results After the removal of outliers and invalid data, the repeatability (RSDr) data was ≤10.0% for all-trans-lutein, ≤12.0% for total lutein, ≤4.2% for all-trans-β-carotene, ≤6.0% for total β-carotene, and 1.6% for total lycopene. Reproducibility (RSDR) was ≤14.8% for all-trans-lutein, ≤19.9% for total lutein, ≤15.3% for all-trans-β-carotene, ≤13.7% for total β-carotene, and 7.4% for total lycopene. Conclusions The repeatability and reproducibility values met the criteria in the Standard Method Performance Requirements (SMPRs) for β-carotene and lycopene and it was recommended that the method be approved as a Final Action for these analytes. Since the method did not meet the SMPR for lutein, it was recommended that it remain a First Action method for this analyte. Highlights AOAC Official MethodSM2016.13 was validated through a collaborative study to be accurate and reproducible for the determination of β-carotene and lycopene in infant formula and adult nutritionals.

Single-Laboratory Validation of AOAC Official Method 2011.10 for Vitamin B12 in ‘Indian’ Infant and Pediatric Formulas and Adult Nutritionals

Background: Ensuring the quality of infant and pediatric formulas and adult nutritionals is of utmost importance for the health and safety of rapidly urbanizing Indian population. B12 is an important water-soluble vitamin, which is fortified externally in such nutritional formulations. The Bureau of Indian Standards (BIS) has a recommended microbiological assay–based method for determination of vitamin B12 that is not precise and accurate enough to meet the label claim requirements of infant, adult, and/or pediatric nutritionals. The AOAC Official Method 2011.10 was originally developed under the AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) for the determination of vitamin B12 in infant and pediatric formulas and adult nutritionals. However, those SPIFAN matrixes did not contain malt and other indigenous cereal and legume flour (with or without cocoa powder), which are commonly found in Indian formulations. Thus, there is a need to replace this method with a more precise and accurate method. Objective: This study was undertaken to validate the AOAC Official Method 2011.10 on vitamin B12 in ‘Indian’ infant and pediatric formulas and adult nutritionals. Methods: The single-laboratory validation (SLV) of AOAC Method 2011.10 was carried out as per the AOAC Guidelines in six Indian pediatric and adult nutritional formulas to verify its fitness for purpose. Cobalamin in the sample was converted to cyanocobalamin on treatment with potassium cyanide. The sample was then subjected to clean up through a C18 cartridge. Vitamin B12 in the eluted extract was separated from other components using size-exclusion column chromatography followed by a C18 column. The HPLC analysis was carried out at 550 nm. Results: Diastase treatment and C18 solid-phase extraction cleanup satisfactorily removed the matrix interference. The relative standard deviation of the determined values in 30 samples each from 6 selected Indian products and NIST SRM 1849a was &lt;20%. The average recoveries for the spiked recovery samples ranged from 91.75 to 101.14%. Conclusions: Method 2011.10 met the standard method performance requirements set forth by the AOAC SPIFAN. Therefore, we recommend the Method 2011.10 for adoption as the BIS official method for the analysis of vitamin B12 in ‘Indian’ infant and pediatric formulas and adult nutritionals. Highlights: This was the first SLV project that the AOAC India section undertook to extend the scope of the AOAC Method 2011.10 for vitamin B12 analysis by validating it in ‘Indian’ infant and pediatric formulas and adult nutritionals.

Determination of Total Folates in Complex Nutritional Drinks and Supplements Using a Tri-Enzyme Microbiological Method and Michaelis-Menten Kinetics

Background: Recent development of LC methods for the determination of total folates (vitamin B9) in complex matrixes have been hindered by vitamer interconversion and yield variability. The official microbiological method (AOAC Official Methods of Analysis 944.12 and 960.46) uses an end point turbidity reading to determine folate concentration. However, when measuring complex matrixes, shifts are observed in the growth curves of the microorganism and inaccuracies are introduced to this quantification method. Objective/Methods: In addition to the tri-enzyme digestion of the standard microbiological method, we have applied enzyme modeling of the initial velocity of bacterial growth using Michaelis-Menten kinetics to achieve more accurate and reproducible determinations of total folates. Results/Conclusions: Accuracy determined through spike recovery in Infant/Adult Nutritional Drink and a complex vitamin matrix gave values acceptable to AOAC standards of 85–110%. Repeatability of the low mass fraction analyte measured at micrograms per 100 g yielded relative standard deviations &lt;15% for all matrixes tested, including three standard reference materials.

Physico-Chemical Analysis of Protein Fortified Papaya Jam

<p><em>Abstract</em> - <strong>Vegetarian food products content less protein source compare to non-vegetarian. Protein fortification is one of the option can be chosen to fulfil protein needs of the vegetarian consumer. Papaya Jam was fortified with protein isolate from soy bean with amount of 5%. The analysis of physico-chemical analysis was done using Association of Official Agricultural Chemist (AOAC) official methods of analysis. The physico-chemical analysis of protein fortified papaya jam showed that the product had 31.07</strong><strong>±</strong><strong> </strong><strong>0.20</strong><strong> % moisture contents, 68.00 </strong><strong>± 0.54 </strong><strong>% Total Soluble Solid, 0.49 </strong><strong>± 0.02</strong><strong> % of acidity, 3.27</strong><strong>± 0.52</strong><strong> in pH, 31.30</strong><strong>± 0.10</strong><strong> mg/100g ascorbic acid content, 53.60 </strong><strong>± 0.18</strong><strong> % total sugar amount, 33.60</strong><strong>± 0.78 </strong><strong>% reducing sugar, 2.81</strong><strong>± 0.08</strong><strong> mg/100 total carotenoid, 0.540.54 </strong><strong>± 0.02</strong><strong> mg/100g total phenol, and 5.43 </strong><strong>± 0.06</strong><strong> % dwb of crude protein content. The physico-chemical of protein fortified papaya jam found still in the range of jam standard standardized by FAO and previous research results for physico-chemical analysis of jam.</strong><strong>ntuk naskah berbahasa Indonesia, abstrak ditulis dalam bahasa Indonesia dan bahasa Inggris.</strong></p><p><strong><em>Keywords</em></strong> - <em>Physico-chemical, protein, fortification, papaya, jam </em></p>

Validation of the Biofish-300 SUL Enzymatic Biosensor for the Detection of Sulfite in Crustacean

Background: Sulfites are some of the oldest and most widespread preservatives in our food supply. They are food additives that have antioxidant properties, but they are also recorded as allergens by the main international regulatory bodies on food safety because of their adverse healtheffect. Hence, sulfites maximum concentration in foodstuff is regulated and they must be ensured by the agro-food processing industries. The most widely used technique for the quantification of sulfites is the Modified Monier-Williams (AOAC Official Method 990.28). Objective:In this method, SO2 is released from sulfites and some bound compounds when the sample is mixed with an acid (normally hydrochloric acid, but sometimes phosphoric acid) and heated. The SO2 is distilled using a stream of nitrogen gas,which carries the gaseous SO2 into an absorbing solution of hydrogen peroxide (H2O2) where it is oxidised to sulphuric acid. The amount of SO2 distilled into the H2O2 is determined by titration with 0.1M sodium hydroxide. Apart from being time consuming (at least 2 h) and the usage of toxicsolvents, the method presents some other disadvantages that make it inappropriate as a routine-controltechnique for the agro-food industry. Hence, the industry demands simple, fast and accurate methods for sulfite level monitoring. Methods: BIOLAN is a SME that develops and commercializesbiosensors for quantitative analysis of food quality and safety parameters, based on its proprietary enzyme-based electrochemical biosensor technology platform. This technology enables high accurate and robust analysis with a compact device that help the users to control the quality in an easy and safety manner. Biofish-300 SUL method is a highly specificenzimatic biosensor for the rapid quantification ofsulfite, measured as SO2 content, in crustaceans. It consists on the extraction of sulfitein an aqueous based solution, by the aid of an Ultra-turrax or similar, and its subsequent quantification by the biosensor after previous calibration (3 min). Results: Sulfite in raw shrimp head-on, raw shrimp head-off, and boiled shrimp was analyzed, and performance was examined using naturally contaminated and spiked samples by comparisons with AOAC Official Methods of AnalysisSM (OMA) 990.28. Linearity, selectivity, matrix, consistency, and robustness were evaluated. All results were within acceptable ranges except robustness, which reflected deviation in the sample volume and ultraturrax time compared with the standard assay procedures describedin the Biofish 300 SUL Instruction Manual. Accuracy, assessed as a comparison of the Biofish results with the OMA results, ranged from 82 to 115% in all samples except for fortified raw shrimp head-on, inwhich the low level yielded an accuracy of 138%. The method bias was in general negative in both incurred and fortified high levels, and slightly positive in incurred low levels. Repeatability was very good as shown by the low RSDr values, demonstrating acceptable repeatability precision with results &lt;10% in most of the evaluated values. Regression analyses showed a good correlation between the Biofish and OMA methods with R2 = 0.99 in all cases. Conclusions: As awhole, accuracy, recovery and bias within range results indicate that the kit provides accurate and precise sulfite quantification for all the evaluated matrices, confirming that sample preparation and assay procedures produce acceptable results. Biofish 300 SUL has proved to be a suitable tool for monitoring sulfite levels in quality control routines dueto its high accuracy, precision, rapid response andease of use. Highlights: With a simple sample preparation, results are obtained in approximately 3 min, making a big difference with other technologies that require specific skills or tedious sample pretreatments and analysis procedures.