Enantioseparations in normal- and reversed-phase nano-high-performance liquid chromatography and capillary electrochromatography using polyacrylamide and polysaccharide derivatives as chiral stationary phases

1999 ◽  
Vol 837 (1-2) ◽  
pp. 51-63 ◽  
Author(s):  
Kerstin Krause ◽  
Marco Girod ◽  
Bezhan Chankvetadze ◽  
Gottfried Blaschke
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Capillary Electrochromatography ◽  
High Performance ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Reversed Phase ◽  
Polysaccharide Derivatives

scholarly journals The Separation of Cannabinoids on Sub-2 µm Immobilized Polysaccharide Chiral Stationary Phases

2021 ◽  
Vol 14 (12) ◽  
pp. 1250
Author(s):  
Takafumi Onishi ◽  
Weston J. Umstead
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Reversed Phase ◽  
Normal Phase ◽  
Elution Order ◽  
Testing Methods ◽  
Structural Isomers

The increased use and applicability of Cannabis and Cannabis-derived products has skyrocketed over the last 5 years. With more and more governing bodies moving toward medical and recreational legalization, the need for robust and reliable analytical testing methods is also growing. While many stationary phases and methods have been developed for this sort of analysis, chiral stationary phases (CSPs) are unique in this area; not only can they serve their traditional chiral separation role, but they can also be used to perform achiral separations. Given that mixtures of cannabinoids routinely contain enantiomers, diastereomers, and structural isomers, this offers an advantage over the strictly achiral-only analyses. This work presents the separation of a 10-cannabinoid mixture on several polysaccharide-based sub-2 µm CSPs with both normal-phase and reversed-phase ultra-high-performance liquid chromatography (UHPLC) conditions. Along with the separation of the mixture, appropriate single-peak identification was performed to determine the elution order and reported where applicable.

Polysaccharide derivatives as useful chiral stationary phases in high-performance liquid chromatography

2007 ◽  
Vol 79 (9) ◽  
pp. 1561-1573 ◽  
Author(s):  
Xiaoming Chen ◽  
Chiyo Yamamoto ◽  
Yoshio Okamoto
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Silica Gel ◽  
Chromatographic Separation ◽  
High Performance ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Separation Of Enantiomers ◽  
Polysaccharide Derivatives ◽  
New Generation

The chromatographic separation of enantiomers using chiral stationary phases (CSPs) has significantly advanced. The esters and carbamates of polysaccharides coated on silica gel have been extensively studied and widely used as CSPs for high-performance liquid chromatography (HPLC). In order to overcome the strict solvent limitation on these coated CSPs, the preparation of a new generation of CSPs consisting of immobilized polysaccharide derivatives has become increasingly important. The universal solvent compatibility of the new CSPs provides flexibility in both analytical and preparative chromatographies.

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