scholarly journals Characterizations of Pneumocystis carinii and rat lung lipids: glyceryl ethers and fatty alcohols

1998 ◽  
Vol 39 (10) ◽  
pp. 1907-1917 ◽  
Author(s):  
Edna S. Kaneshiro ◽  
Zengkui Guo ◽  
Donggeun Sul ◽  
Kathryn A. Kallam ◽  
Koka Jayasimhulu ◽  
...  
Keyword(s):  
Pneumocystis Carinii ◽  
Fatty Alcohols ◽  
Rat Lung

scholarly journals Down-Regulation of GATA-2 Transcription duringPneumocystis carinii Infection

2000 ◽  
Vol 68 (8) ◽  
pp. 4720-4724 ◽  
Author(s):  
Xing Tang ◽  
Mark E. Lasbury ◽  
Darrell D. Davidson ◽  
Marilyn S. Bartlett ◽  
James W. Smith ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Pneumocystis Carinii ◽  
Pcr Primers ◽  
Northern Hybridization ◽  
Rat Lung ◽  
Bronchial Epithelial ◽  
Rat Lungs ◽  
Rna Probes

ABSTRACT Differences in gene expression between Pneumocystis carinii-infected and noninfected rats were examined. Total RNA was isolated from homogenized rat lungs and then subjected to differential display with combinations of oligo(dT) and various arbitrary PCR primers. Approximately 50 differentially expressed bands were observed. Several of these DNA bands were isolated, reamplified, and cloned. The cloned DNA fragments were used as probes to perform Northern hybridization on RNA from P. carinii-infected and noninfected rat lungs. One clone was found to react with a 3-kb mRNA from noninfected but not from P. carinii-infected rat lung, suggesting that the gene represented by this clone was down-regulated during P. carinii infection. The nucleotide sequence of this clone was determined and found to be 97% homologous to the mouse GATA-2 transcription factor. In situ hybridization using RNA probes derived from this clone revealed that alveolar macrophages, resident lung monocytes, and bronchial epithelial cells express the GATA-2 gene in the lung.

Effect of Proteolytic Enzymes on Pneumocystis carinii in Rat Lung Tissue

1981 ◽  
Vol 67 (2) ◽  
pp. 196 ◽  
Author(s):  
Benjamin J. Stahr ◽  
Peter D. Walzer ◽  
Kokichi Yoneda
Keyword(s):  
Lung Tissue ◽  
Proteolytic Enzymes ◽  
Pneumocystis Carinii ◽  
Rat Lung

Ultrastructural Observations of Pneumocystis carinii Attachment to Rat Lung

1985 ◽  
Vol 151 (1) ◽  
pp. 181-186 ◽  
Author(s):  
N. G. Henshaw ◽  
J. L. Carson ◽  
A. M. Collier
Keyword(s):  
Pneumocystis Carinii ◽  
Rat Lung

Immunogold labeling of Pneumocystis carinii for Electron microscopy

1991 ◽  
Vol 49 ◽  
pp. 270-271
Author(s):  
Michael P. Goheen ◽  
Marilyn S. Bartlett ◽  
James W. Smith
Keyword(s):  
Electron Microscopy ◽  
Pneumocystis Carinii ◽  
Severe Pneumonia ◽  
Culture Fluid ◽  
Immunogold Labeling ◽  
Antigenic Sites ◽  
Transmission Electron ◽  
Response To Chemotherapy ◽  
Acquired Immunodeficiency ◽  
Immunodeficiency Syndrome

Studies of the biology of Pneumocystis carinii (PC) are of increasing importance because this extracellular pathogen is a frequent source of severe pneumonia in patients with acquired immunodeficiency syndrome (AIDS) and is a leading cause of mortality in these patients. Immunoelectron microscopic localization of antigenic sites on the surface of PC would improve the understanding of these sites and their role in pathenogenisis of the disease and response to chemotherapy. The purpose of this study was to develop a methodology for visualizing immunoreactive sites on PC with transmission electron microscopy (TEM) using immunogold labeled probes.Trophozoites of PC were added to spinner flask cultures and allowed to grow for 7 days, then aliquots of tissue culture fluid were centrifuged at 12,000 RPM for 30 sec. Pellets of organisims were fixed in either 1% glutaraldehyde, 0.1% glutaraldehyde-4% paraformaldehyde, or 4% paraformaldehyde for 4h. All fixatives were buffered with 0.1M Na cacodylate and the pH adjusted to 7.1. After fixation the pellets were rinsed in 0.1M Na cacodylate (3X), dehydrated with ethanol, and immersed in a 1:1 mixture of 95% ethanol and LR White resin.

Replication of Internal Biological Surfaces

1972 ◽  
Vol 30 ◽  
pp. 308-309
Author(s):  
J. A. Nowell ◽  
J. Pangborn ◽  
W. S. Tyler
Keyword(s):  
Da Vinci ◽  
Leonardo Da Vinci ◽  
Rat Lung ◽  
Tubular Structures ◽  
Cerebral Ventricles ◽  
Internal Surfaces ◽  
Biological Surfaces ◽  
Dog Kidney ◽  
Lung And Kidney ◽  
Scanning Electron

Leonardo da Vinci in the 16th century, used injection replica techniques to study internal surfaces of the cerebral ventricles. Developments in replicating media have made it possible for modern morphologists to examine injection replicas of lung and kidney with the scanning electron microscope (SEM). Deeply concave surfaces and interrelationships to tubular structures are difficult to examine with the SEM. Injection replicas convert concavities to convexities and tubes to rods, overcoming these difficulties.Batson's plastic was injected into the renal artery of a horse kidney. Latex was injected into the pulmonary artery and cementex in the trachea of a cat. Following polymerization the tissues were removed by digestion in concentrated HCl. Slices of dog kidney were aldehyde fixed by immersion. Rat lung was aldehyde fixed by perfusion via the trachea at 30 cm H2O. Pieces of tissue 10 x 10 x 2 mm were critical point dried using CO2. Selected areas of replicas and tissues were coated with silver and gold and examined with the SEM.

Comparative morphology of Pneumocystis Carinii in Situ and after bronchoalveolar lavage

1986 ◽  
Vol 44 ◽  
pp. 244-245
Author(s):  
C. A. Itatani ◽  
A. Hing ◽  
W. Jackson ◽  
G.J. Marshall
Keyword(s):  
Bronchoalveolar Lavage ◽  
Pneumocystis Carinii ◽  
Comparative Morphology

Pneumocystis carinii (PC) is an organism capable of causing fatal pneumonia in immune suppressed individuals and has recently gained prominence because of its association with AIDS. A similar organism occurs in rats and infection may be induced with cortisone injections. In order to isolate PC for further study bronchoal veol ar lavage (BAL) was performed. Differences in the ul trastructure of BAL-obtained organisms and PC in situ were observed and are herein reported.

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