High frequency of constitutive alkali-labile sites in mouse major satellite DNA, detected by DNA breakage detection-fluorescence in situ hybridization

Variation in the location of the 15p region D15Z1 is recognized as a polymorphism in several human populations. We used high-stringency Fluorescence In Situ Hybridization (FISH) to detect D15Z1 in a Mexican cohort. Here, we report the presence of extra D15Z1 sequences on the p-arm of acrocentric chromosomes other than 15 in two groups of Mexican couples, one with healthy offspring (n = 75) and the other with aneuploid offspring (n = 87), mainly trisomy 21. The additional D15Z1 polymorphism was significantly increased in individuals with aneuploid offspring (26.4%), in comparison to individuals with healthy offspring (14%). The most frequent acceptor chromosome of D15Z1 was chromosome 13p, followed by 14p, and finally, 21p. Our results show an overall frequency of 21.6% of this polymorphism in the Mexican population and suggest that its presence might be associated with the mis-segregation of other acrocentric chromosomes and aneuploid offspring. The high frequency of the polymorphism of the D15Z1 sequence on acrocentric chromosomes other than 15 suggests a sequence homogenization of the acrocentric p arms, related to the important function of the centromere and the nucleolar organization region, which flank satellite III DNA.

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Molecular cytogenetics of α satellite DNA from chromosome 12: fluorescence in situ hybridization and description of DNA and array length polymorphisms

Cytogenetic and Genome Research ◽

10.1159/000133071 ◽

1991 ◽

Vol 56 (3-4) ◽

pp. 144-148 ◽

Cited By ~ 12

Author(s):

G.M. Greig ◽

S. Parikh ◽

J. George ◽

V.E. Powers ◽

H.F. Willard

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Satellite Dna ◽

Molecular Cytogenetics ◽

Chromosome 12 ◽

Alpha Satellite ◽

Alpha Satellite Dna ◽

Length Polymorphisms

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Fluorescence in situ hybridization (FISH) of a whole-arm translocation involving chromosomes 18 and 20 with α-satellite DNA probes: Detection of a centromeric DNA break?

American Journal of Medical Genetics ◽

10.1002/ajmg.1320440314 ◽

1992 ◽

Vol 44 (3) ◽

pp. 340-344 ◽

Cited By ~ 9

Author(s):

Eduardo S. Cantú ◽

Tasneem A. Khan ◽

G. Shashidhar Pai

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Satellite Dna ◽

Dna Probes ◽

Centromeric Dna ◽

Dna Break

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Isolation and characterization of a satellite DNA family in the Saccharum complex

Genome ◽

10.1139/g98-076 ◽

1998 ◽

Vol 41 (6) ◽

pp. 854-864 ◽

Cited By ~ 25

Author(s):

Karine Alix ◽

Franc-Christophe Baurens ◽

Florence Paulet ◽

Jean-Christophe Glaszmann ◽

Angélique D'Hont

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Satellite Dna ◽

Repetitive Sequences ◽

Repeat Sequence ◽

Miscanthus Sinensis ◽

Saccharum Complex ◽

Satellite Dnas ◽

Isolation And Characterization

EaCIR1, a 371-bp Erianthus-specific satellite DNA sequence, was cloned from TaqI restricted genomic DNA after agarose-gel electrophoresis. This sequence has 77% homology with a 365-bp satellite of Helictotrichon convolutum and 72% homology with a 353-bp tandem repeat sequence from Oryza sativa. PCR primers defined in the conserved regions of these repetitive sequences were used to isolate other satellite DNAs in different representatives of the Saccharum complex: SoCIR1 in Saccharum officinarum, SrCIR1 in Saccharum robustum, SsCIR1 and SsCIR2 in Saccharum spontaneum, and MsCIR1 in Miscanthus sinensis. EaCIR1 and SoCIR1 were localized to subtelomeric regions of the chromosomes by fluorescence in situ hybridization. Southern hybridization experiments, using two representatives of this repeat sequence family as probes, illustrated contrasting species-specificity and demonstrated the existence of similar repetitive elements in sorghum and maize.Key words: satellite DNA, sugarcane, Saccharum complex, Gramineae, fluorescence in situ hybridization, FISH.

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