Self-association versus interassociation in hydrogen bonded polymer blends: 1. Determination of equilibrium constants from miscible poly(2,6-dialkyl-4-vinyl phenol) blends

This article reviews the progress of a personal endeavour to develop chromatography as a quantitative procedure for the determination of reaction stoichiometries and equilibrium constants governing protein interactions. As well as affording insight into an aspect of chromatography with which many protein chemists are unfamiliar, it shows the way in which minor adaptations of conventional chromatographic practices have rendered the technique one of the most powerful methods available for the characterization of interactions. That pathway towards quantification is followed from the introduction of frontal gel filtration for the study of protein self-association to the characterization of ligand binding by the biosensor variant of quantitative affinity chromatography.

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Thermodynamic studies on subunit assembly in human hemoglobin. Self-association of oxygenated chains (alphaSH and betaSH): determination of stoichiometries and equilibrium constants as a function of temperature.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)32800-4 ◽

1977 ◽

Vol 252 (1) ◽

pp. 74-81 ◽

Cited By ~ 5

Author(s):

R Valdes ◽

G K Ackers

Keyword(s):

Equilibrium Constants ◽

Human Hemoglobin ◽

Thermodynamic Studies ◽

Subunit Assembly ◽

Self Association

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Determination of Self-Association Equilibrium Constants of Ethanol by FTIR Spectroscopy

The Journal of Physical Chemistry ◽

10.1021/jp9613448 ◽

1996 ◽

Vol 100 (50) ◽

pp. 19268-19272 ◽

Cited By ~ 26

Author(s):

Fanny Schwager ◽

Eva Marand ◽

Richey M. Davis

Keyword(s):

Ftir Spectroscopy ◽

Equilibrium Constants ◽

Self Association ◽

Association Equilibrium

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Prediction of the Phase Behaviour of Hydrogen-Bonded Polymer Blends

Australian Journal of Chemistry ◽

10.1071/ch06132 ◽

2006 ◽

Vol 59 (8) ◽

pp. 499 ◽

Cited By ~ 3

Author(s):

Michael M. Coleman ◽

Paul C. Painter

Keyword(s):

Polymer Blends ◽

Functional Group ◽

Equilibrium Constants ◽

Phase Behaviour ◽

Low Molecular Weight ◽

Full Circle ◽

Energy Of Mixing ◽

The Subject ◽

Free Energy Of Mixing ◽

Hydrogen Bonded

In the early 1990s your authors believed that they had essentially solved the problem of predicting the phase behaviour of hydrogen-bonded polymer blends. A text devoted to this subject, Specific Interactions and the Miscibility of Polymer Blends, was published and we thought that it was now time to look around for something else to do. This was before a colleague, Boris Veytsman, pointed out that there was a flaw in our derivation of the free energy of mixing equation. It has taken us some 15 years to correct the theory and match the predictions of the phase behaviour of hydrogen-bonded blends that we presented in our 1991 book. So we have come full circle. The subject has become far more complicated, but at the same time far more interesting. Along the way we have discovered that the phase behaviour of hydrogen-bonded polymer blends can be successfully predicted using equilibrium constants determined from appropriate low molecular weight analogues, if chain connectivity effects such as intermolecular screening and functional group accessibility are included.

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