Distribution of the hyaluronate lyase encoding gene hylB and the insertion element is1548 in streptococci of serological group B isolated from animals and humans

2002 ◽  
Vol 73 (2) ◽  
pp. 131-135 ◽  
Author(s):  
A.Ö Yildirim ◽  
K Fink ◽  
Ch Lämmler
Keyword(s):  
Insertion Element ◽  
Serological Group ◽  
Hyaluronate Lyase ◽  
Encoding Gene ◽  
Group B

scholarly journals A unique serine-rich repeat protein (Srr-2) and novel surface antigen (ε) associated with a virulent lineage of serotype III Streptococcus agalactiae

Microbiology ◽  
2006 ◽  
Vol 152 (4) ◽  
pp. 1029-1040 ◽  
Author(s):  
Kyle N. Seifert ◽  
Elisabeth E. Adderson ◽  
April A. Whiting ◽  
John F. Bohnsack ◽  
Paula J. Crowley ◽  
...  
Keyword(s):  
Surface Antigen ◽  
Invasive Disease ◽  
Group B Streptococci ◽  
Gene Encoding ◽  
Repeat Protein ◽  
Encoding Gene ◽  
Group B ◽  
Mouse Model Of Infection ◽  
Highly Virulent

Group B streptococci (GBS) are pathogens of both neonates and adults, with serotype III strains in particular being associated with invasive disease and meningitis. In this study, a novel GBS surface antigen, ε, was found to be co-expressed with the previously reported δ antigen on an identical subset of serotype III GBS. Expression of δ/ε on the surface of serotype III GBS was shown to distinguish the restriction digest pattern (RDP) III-3 and multilocus sequence typing (ST)-17 lineage. ε-Specific antibodies were reactive with a unique, high-molecular-mass, serine-rich repeat protein (Srr-2) found exclusively in RDP III-3 strains. The gene encoding Srr-2 was located within a putative accessory secretory locus that included secY2 and secA2 homologues and had a genetic organization similar to that of the secY2/A2 locus of staphylococci. In contrast, serotype III δ/ε-negative strains and strains representative of serotypes Ia, Ib, Ic and II shared a common Srr-encoding gene, srr-1, and an organization of the secY2/A2 locus similar to that of previously reported serotype Ic, δ/ε-negative serotype III and serotype V GBS strains. Representative serotype III δ/ε-positive strains had LD90 values 3–4 logs less than those of serotype III δ/ε-negative strains in a neonatal mouse model of infection. These results indicate that the RDP III-3/ST-17 lineage expresses Srr-2 and is highly virulent in an in vivo model of neonatal sepsis.

Characterization of the Group B Streptococcal Hyaluronate Lyase

1994 ◽  
Vol 315 (2) ◽  
pp. 431-437 ◽  
Author(s):  
D.G. Pritchard ◽  
B. Lin ◽  
T.R. Willingham ◽  
J.R. Baker
Keyword(s):  
Hyaluronate Lyase ◽  
Group B

Phase variation in streptococci of serological group B

Apmis ◽  
1994 ◽  
Vol 102 (7-12) ◽  
pp. 925-930 ◽  
Author(s):  
S. ISRINA OCTAVIA SALASIA ◽  
I. WAYAN TEGUH WIBAWAN ◽  
CHRISTOPH LÄMMLER ◽  
MATS SELLIN
Keyword(s):  
Phase Variation ◽  
Serological Group ◽  
Group B

scholarly journals Cloning and expression of the gene for group B streptococcal hyaluronate lyase.

1994 ◽  
Vol 269 (48) ◽  
pp. 30113-30116
Author(s):  
B Lin ◽  
S K Hollingshead ◽  
J E Coligan ◽  
M L Egan ◽  
J R Baker ◽  
...  
Keyword(s):  
Cloning And Expression ◽  
Hyaluronate Lyase ◽  
Group B

scholarly journals The Putative Glycosyltransferase-Encoding Gene cylJ and the Group B Streptococcus (GBS)-Specific Gene cylK Modulate Hemolysin Production and Virulence of GBS

2007 ◽  
Vol 75 (4) ◽  
pp. 2063-2066 ◽  
Author(s):  
Marie-Pierre Forquin ◽  
Asmae Tazi ◽  
Manuel Rosa-Fraile ◽  
Claire Poyart ◽  
Patrick Trieu-Cuot ◽  
...  
Keyword(s):  
Group B Streptococcus ◽  
Transposon Mutagenesis ◽  
Cytolytic Activity ◽  
Specific Gene ◽  
Pigment Formation ◽  
System A ◽  
Encoding Gene ◽  
Group B

ABSTRACT Group B streptococcus (GBS) expresses a hemolysin/cytolysin that plays an important role in pathogenesis. Using the Himar1 transposon mutagenesis system, a hypohemolytic mutant carrying an interrupted cylJ gene was characterized. cylJ, encoding a putative glycosyltransferase, and cylK, whose product is unknown, are both required for the full hemolytic/cytolytic activity, pigment formation, and virulence of GBS.

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