In Vivo and in Vitro Properties of a Temperature Sensitive Mutant of Infectious Bovine Rhinotracheitis Virus

1974 ◽  
Vol 16 (3) ◽  
pp. 328-335 ◽  
Author(s):  
N. Zygraich ◽  
Michèle Lobmann ◽  
E. Vascoboinic ◽  
E. Berge ◽  
C. Huygelen
Keyword(s):  
Temperature Sensitive ◽  
Infectious Bovine Rhinotracheitis ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Infectious Bovine Rhinotracheitis Virus

Directionality of microtubule assembly: an in vivo study with the ciliate Tetrahymena

1978 ◽  
Vol 33 (1) ◽  
pp. 227-234
Author(s):  
S.F. Ng
Keyword(s):  
Tetrahymena Thermophila ◽  
Recessive Gene ◽  
Microtubule Assembly ◽  
In Vivo Study ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Posterior Portion ◽  
Temperature Sensitive Mutant

A temperature-sensitive mutant homozygous for the recessive gene molb in Tetrahymena thermophila offers opportunity for studying the direction of microtubule assembly in vivo. At 39 degrees C the mutant fails to divide properly; the 2 daughter animals remain attached and bend over each other. As revealed by protargol staining, the bending results in acute turning and breaking of some of the longitudinal microtubular bands close and parallel to the surface. Hence, 2 broken microtubular ends are available for study of the problem of directionality of microtubule assembly, by assessing which of the 2 ends regenerates. In most cases the posterior portion of the longitudinal microtubular band regenerates. The present study hence supports the conclusion based on in vitro observation in other systems that microtubule assembly is predominantly unidirectional.

Sign in / Sign up

Export Citation Format

Share Document