Effects of Binding of Ligand (FVIIa) to Induced Tissue Factor in Human Endothelial Cells

2000 ◽  
Vol 98 (4) ◽  
pp. 311-321 ◽  
Author(s):  
Merete T. Wiiger ◽  
Serena Pringle ◽  
Kjell Sverre Pettersen ◽  
Nobuhiro Narahara ◽  
Hans Prydz
Keyword(s):  
Endothelial Cells ◽  
Tissue Factor ◽  
Human Endothelial Cells

Uremic Medium Disturbs the Hemostatic Balance of Cultured Human Endothelial Cells

2001 ◽  
Vol 86 (10) ◽  
pp. 1099-1105 ◽  
Author(s):  
Mireia Serradell ◽  
Aleix Cases ◽  
María Zurbano ◽  
José Aznar-Salatti ◽  
José López-Pedret ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Tissue Factor ◽  
Northern Blot Analysis ◽  
Human Endothelial Cells ◽  
Perfusion Chamber ◽  
Uremic Serum ◽  
Incubation Periods ◽  
Platelet Deposition ◽  
Accelerated Growth ◽  
Chamber Exposure

SummaryWe have investigated the ability of serum from uremic patients to modify the thrombogenic properties of the endothelium. The effects of uremic medium on the morphology of endothelial cells (ECs), and their resistance to flow was analyzed. The influence of uremic media on the reactivity of the extracellular matrix (ECM) generated by ECs towards normal platelets was evaluated in a parallel-plate perfusion chamber. Exposure of ECs to uremic medium resulted in abnormal cell morphology and signs of an accelerated growth. Detachment of ECs exposed to circulating blood was increased when cells had been grown with media supplemented with uremic serum (21% vs. 14% non exposed). Platelet deposition was significantly elevated on ECMs generated in the presence of uremic media (uremicECMs) (p<0.01 vs. control studies). Effects of uremic serum were not observed at short incubation periods (5 h) but were evident after 24 or 72 h of incubation. Northern blot analysis revealed increased expression of tissue factor (TF) mRNA in ECs exposed to uremic conditions. Immunocytochemical methods detected an augmented expression of TF antigen on uremic ECMs. Incubation of ECMs with an antibody to human tissue factor prevented the increase in platelet deposition observed in uremic ECMs, suggesting that the presence of TF in ECM could be responsible for the enhanced platelet deposition. Results from our study indicate that uremic medium impairs the antithrombotic functions of cultured endothelial cells.

scholarly journals Statin Prevents Tissue Factor Expression in Human Endothelial Cells

Circulation ◽  
2002 ◽  
Vol 105 (15) ◽  
pp. 1756-1759 ◽  
Author(s):  
Masato Eto ◽  
Toshiyuki Kozai ◽  
Francesco Cosentino ◽  
Hana Joch ◽  
Thomas F. Lüscher
Keyword(s):  
Endothelial Cells ◽  
Tissue Factor ◽  
Tissue Factor Expression ◽  
Human Endothelial Cells ◽  
Factor Expression

Malformin-a1 inhibits the binding of interleukin-1β (il1β) and suppresses the expression of tissue factor in human endothelial cells and monocytes

1994 ◽  
Vol 48 (6) ◽  
pp. 1211-1217 ◽  
Author(s):  
Jean-Marc Herbert ◽  
Pierre Savi ◽  
Alain Lalé ◽  
Marie-Claude Laplace ◽  
Nicole Baudry ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Tissue Factor ◽  
Interleukin 1Β ◽  
Human Endothelial Cells ◽  
Malformin A1

Modification of Tissue-Factor mRNA and Protein Response to Thrombin and Interleukin 1 by High Glucose in Cultured Human Endothelial Cells

Diabetes ◽  
1989 ◽  
Vol 38 (2) ◽  
pp. 212-218 ◽  
Author(s):  
D. Boeri ◽  
F. E. Almus ◽  
M. Maiello ◽  
E. Cagliero ◽  
L. V. M. Rao ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Tissue Factor ◽  
High Glucose ◽  
Interleukin 1 ◽  
Human Endothelial Cells ◽  
Protein Response

Evaluation of tissue-factor production by human endothelial cells incubated with three acrylic bone cements

2001 ◽  
Vol 55 (1) ◽  
pp. 131-136 ◽  
Author(s):  
Elisabetta Cenni ◽  
Gabriela Ciapetti ◽  
Donatella Granchi ◽  
Lucia Savarino ◽  
Susanna Stea ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Tissue Factor ◽  
Bone Cements ◽  
Human Endothelial Cells ◽  
Factor Production

scholarly journals Induction of tissue factor activity in endothelial cells and monocytes by a modified form of albumin present in normal human plasma

Blood ◽  
1992 ◽  
Vol 79 (11) ◽  
pp. 2888-2895
Author(s):  
KJ Faucette ◽  
CJ Parker ◽  
T McCluskey ◽  
NJ Bernshaw ◽  
GM Rodgers
Keyword(s):  
Endothelial Cells ◽  
Human Plasma ◽  
Tissue Factor ◽  
Tissue Factor Expression ◽  
Factor Activity ◽  
Normal Human Plasma ◽  
Human Endothelial Cells ◽  
Tissue Factor Activity ◽  
Factor Expression ◽  
Normal Human

Molecules that induce tissue factor expression by responsive cells such as endothelial cells and monocytes may be important in the regulation of hemostasis and, perhaps, in mediating certain hemostatic disorders. A constituent of normal human plasma capable of inducing tissue factor activity in human endothelial cells and monocytes has been isolated and identified as a derivative of, or modification associated with albumin. Procoagulant albumin caused a concentration-dependent induction of tissue factor expression by human endothelial cells, but bovine endothelial cells were unresponsive. The dose-response curve developed a plateau phase, indicating that the capacity of endothelial cells to respond to the stimulus was finite. The maximum response induced by the procoagulant albumin was similar to that observed for maximally effective concentrations of endotoxin, interleukin-1, and tumor necrosis factor. Time-course studies showed that procoagulant albumin produced peak activity in 4 to 6 hours. Identification of a procoagulant form of albumin in normal human plasma suggests a potential role for this constituent in regulation of hemostasis.

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