Chapter 46: Cholinergic receptor subtypes and REM sleep in animals and normal controls

Microinjecting cholinomimetics into the pontine reticular formation produces a state that resembles natural rapid eye movement (REM) sleep. Evocation of this REM sleeplike states is anatomically site dependent within the pons and is mediated by muscarinic receptors. The cellular and molecular mechanisms underlying cholinergic REM sleep generation and muscarinic receptor subtype involvement remain to be specified. This study tested the hypothesis that muscarinic receptor subtypes are differentially distributed within the oral and caudal divisions of rat pontine reticular nucleus. In vitro receptor autoradiography was used to localize and quantify M1, M2, and M3 binding sites in the pontine reticular formation and in pontine brain stem regions known to regulate REM sleep. M1-M3 binding sites were present in some REM sleep-related nuclei, such as dorsal raphe and locus ceruleus. The pontine reticular formation was found to have a homogeneous distribution of M2 binding sites across its rostral to caudal extent, indicating that anatomic specificity of cholinergic REM sleep induction cannot be accounted for by a differential density of muscarinic receptors.

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Muscarinic cholinergic receptor subtypes in the hippocampus of aged rats

Mechanisms of Ageing and Development ◽

10.1016/s0047-6374(01)00353-0 ◽

2002 ◽

Vol 123 (5) ◽

pp. 521-528 ◽

Cited By ~ 15

Author(s):

Seyed Khosrow Tayebati ◽

Francesco Amenta ◽

Dahuk El-Assouad ◽

Damiano Zaccheo

Keyword(s):

Cholinergic Receptor ◽

Receptor Subtypes ◽

Aged Rats ◽

Muscarinic Cholinergic Receptor ◽

Muscarinic Cholinergic

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A STUDY OF MUSCARINIC CHOLINERGIC RECEPTOR SUBTYPES IN HUMAN DETRUSOR MUSCLE USING RADIOLIGAND BINDING TECHNIQUES

The Japanese Journal of Urology ◽

10.5980/jpnjurol1989.84.1255 ◽

1993 ◽

Vol 84 (7) ◽

pp. 1255-1261 ◽

Cited By ~ 6

Author(s):

Shun Kondo ◽

Takashi Morita

Keyword(s):

Radioligand Binding ◽

Cholinergic Receptor ◽

Receptor Subtypes ◽

Detrusor Muscle ◽

Muscarinic Cholinergic Receptor ◽

Muscarinic Cholinergic ◽

Human Detrusor

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Localization of muscarinic cholinergic receptor subtypes in human epidermis and keratinocyte cell culture

Journal of Dermatological Science ◽

10.1016/s0923-1811(98)83637-2 ◽

1998 ◽

Vol 16 ◽

pp. S107

Author(s):

Assane Ndoye ◽

Rico Buchli ◽

Brandon M. Greenberg ◽

Vu Thuong Nguyen ◽

Monica A. Lawry ◽

...

Keyword(s):

Cell Culture ◽

Cholinergic Receptor ◽

Human Epidermis ◽

Receptor Subtypes ◽

Muscarinic Cholinergic Receptor ◽

Keratinocyte Cell ◽

Muscarinic Cholinergic

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RT-PCR reveals muscarinic acetylcholine receptor mRNA in the pre-Bötzinger complex

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.2001.281.6.l1420 ◽

2001 ◽

Vol 281 (6) ◽

pp. L1420-L1424 ◽

Cited By ~ 14

Author(s):

Jiunu Lai ◽

Xuesi M. Shao ◽

Richard W. Pan ◽

Edward Dy ◽

Cindy H. Huang ◽

...

Keyword(s):

Muscarinic Receptor ◽

Muscarinic Receptors ◽

Respiratory Rhythm ◽

Pcr Amplification ◽

Cholinergic Receptor ◽

Receptor Subtype ◽

Receptor Subtypes ◽

Muscarinic Receptor Subtypes ◽

Rt Pcr ◽

Bötzinger Complex

Muscarinic receptors mediate the postsynaptic excitatory effects of acetylcholine (ACh) on inspiratory neurons in the pre-Bötzinger complex (pre-BötC), the hypothesized site for respiratory rhythm generation. Because pharmacological tools for identifying the subtypes of the muscarinic receptors that underlie these effects are limited, we probed for mRNA for these receptors in the pre-BötC. We used RT-PCR to determine the expression of muscarinic receptor subtypes in tissue punches of the pre-BötC taken from rat medullary slices. Cholinergic receptor subtype M2 and M3 mRNAs were observed in the first round of PCR amplification. All five subtypes, M1–M5, were observed in the second round of amplification. Our results suggest that the majority of muscarinic receptor subtypes in the pre-BötC are M2 and M3, with minor expression of M1, M4, and M5.

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