Quantitative analysis of vitamin D using m/MALDI-TOF mass spectrometry based on a parylene matrix chip

Vitamin D deficiency is associated with various disorders and is diagnosed based on the concentration of 25-hydroxy vitamin D3 (25(OH)D3) in serum. The parylene matrix chip was fabricated to reduce the matrix background noise, and the homogenous distribution of the matrix was retained for the quantitative analysis of 25(OH)D3. The Amplex Red assay was performed to confirm that the sample-matrix mixing zone of the parylene matrix chip was formed below the surface of the parylene-N film. The homogeneous distribution of the matrix was verified from the fluorescence image. For effective analysis using a parylene matrix chip, 25(OH)D3 was modified through the nucleophilic addition of betaine aldehyde (BA) to form a hemiacetal salt. Such modified 25(OH)D3 with a positive charge from BA could be effectively analyzed using MALDI-TOF mass spectrometry. Serum 25(OH)D3 was extracted by liquid–liquid extraction (LLE) and quantified using MALDI-TOF mass spectrometry based on the parylene matrix chip. The intensity of the mass peak of 25(OH)D3 was linearly correlated (r2 = 0.992) with the concentration of 25(OH)D3 spiked in serum, and the LOD was 0.0056 pmol/μL. Energy drinks and vitamin D3 tablets were also employed for the real sample analysis. Finally, the results of the chemiluminescence binding assay and MALDI-TOF mass spectrometry were statistically analyzed to determine the applicability of the method using the Bland–Altman test and Passing–Bablok regression.

Biotite supports long-range diffusive transport in dissolution–precipitation creep in halite through small porosity fluctuations

Phyllosilicates are generally regarded to have a reinforcing effect on chemical compaction by dissolution–precipitation creep (DPC) and thereby influence the evolution of hydraulic rock properties relevant to groundwater resources and geological repositories as well as fossil fuel reservoirs. We conducted oedometric compaction experiments on layered NaCl–biotite samples to test this assumption. In particular, we aim to analyse slow chemical compaction processes in the presence of biotite on the grain scale and determine the effects of chemical and mechanical feedbacks. We used time-resolved (4-D) microtomographic data to capture the dynamic evolution of the porosity in layered NaCl–NaCl/biotite samples over 1619 and 1932 h of compaction. Percolation analysis in combination with advanced digital volume correlation techniques showed that biotite grains influence the dynamic evolution of porosity in the sample by promoting a reduction of porosity in their vicinity. However, the lack of preferential strain localisation around phyllosilicates and a homogeneous distribution of axial shortening across the sample suggests that the porosity reduction is not achieved by pore collapse but by the precipitation of NaCl sourced from outside the NaCl–biotite layer. Our observations invite a renewed discussion of the effect of phyllosilicates on DPC, with a particular emphasis on the length scales of the processes involved. We propose that, in our experiments, the diffusive transport processes invoked in classical theoretical models of DPC are complemented by chemo-mechanical feedbacks that arise on longer length scales. These feedbacks drive NaCl diffusion from the marginal pure NaCl layers into the central NaCl–biotite mixture over distances of several hundred micrometres and several grain diameters. Such a mechanism was first postulated by Merino et al. (1983).

A Two-Step Approach to Tune the Micro and Nanoscale Morphology of Porous Niobium Oxide to Promote Osteointegration

We present a two-step surface modification process to tailor the micro and nano morphology of niobium oxide layers. Niobium was firstly anodized in spark regime in a Ca- and P-containing solution and subsequently treated by acid etching. The effects of anodizing time and applied potential on the surface morphology is investigated with SEM and AFM, complemented by XPS compositional analysis. Anodizing with a limiting potential of 250 V results in the fast growth of oxide layers with a homogeneous distribution of micro-sized pores. Cracks are, however, observed on 250 V grown layers. Limiting the anodizing potential to 200 V slows down the oxide growth, increasing the anodizing time needed to achieve a uniform pore coverage but produces fracture-free oxide layers. The surface nano morphology is further tuned by a subsequent acid etching process that leads to the formation of nano-sized pits on the anodically grown oxide surface. In vitro tests show that the etching-induced nanostructure effectively promotes cell adhesion and spreading onto the niobium oxide surface.

Immunophenotype of the inflammatory response in the central and enteric nervous systems of cockatiels (Nymphicus hollandicus) experimentally infected with parrot bornavirus 2

Proventricular dilatation disease is a lethal disease of psittacine birds. In this study, we characterized the local cellular immune response in the brain, proventriculus, and small intestine of 27 cockatiels ( Nymphicus hollandicus) experimentally infected with parrot bornavirus 2 (PaBV-2). Perivascular cuffs in the brain were composed of CD3+ T-lymphocytes and Iba1+ macrophages/microglia in most cockatiels (n = 26). In the ganglia of the proventriculus, CD3+ T-lymphocytes (n = 17) and Iba1+ macrophages (n = 13) prevailed. The ganglia of the small intestine had a more homogeneous distribution of these leukocytes, including PAX5+ B-lymphocytes (n = 9), CD3+ T-lymphocytes (n = 8), and Iba1+ macrophages (n = 8). Our results indicate that perivascular cuffs in the brain and the inflammatory infiltrate in the proventriculus of PaBV-2-infected cockatiels is predominately composed of T-lymphocytes, while the inflammatory infiltrates in the ganglia of the small intestine are characterized by a mixed infiltrate composed of T-lymphocytes, B-lymphocytes, and macrophages.

Effect of Cerium and Magnesium on Surface Microcracks of Al–20Si Alloys Induced by High-Current Pulsed Electron Beam

The effect of Ce and Mg on surface microcracks of Al–20Si alloys induced via high-current pulsed electron beam (HCPEB) was studied. Mg was revealed to refine the primary Si phase in the pristine microstructure by forming a Mg2Si phase, leading to the suppression of microcrack propagation within the brittle phase after HCPEB irradiation. The incorporation of Ce into the Al–Si–Mg alloys further refined the primary Si phase and reduced the local stress concentration in the brittle phase induced by HCPEB irradiation. Ultimately, the surface microcracks were observed to be eliminated by the synergistic effects between the two elements. For Al–20Si–5Mg–0.7Ce alloys, Ce demonstrated a homogeneous distribution in the Al matrix on the HCPEB-irradiated alloy surface, while the Mg and Si exhibited a certain degree of aggregation in the Mg2Si phase. Metastable structures were formed on the HCPEB-irradiated alloy surface, including the nano-primary silicon phase, nano-cellular aluminium structure, and nano-Mg2Si phase. Compared with alloy specimens containing Mg, the Al–20Si–5Mg–0.7Ce alloy specimens exhibited an excellent anticorrosion property after HCPEB irradiation mainly due to the combined effects of the grain refinement and microcrack elimination.

Source Apportionment and Geographic Distribution of Heavy Metals and as in Soils and Vegetables Using Kriging Interpolation and Positive Matrix Factorization Analysis

Food security and cultivated land utilization can be seriously affected by heavy metal (HM) pollution of the soil. Therefore, identifying the pollution sources of farmland is the way to control soil pollution and enhance soil quality effectively. In this research, 95 surface soil samples, 34 vegetable samples, 27 irrigation water samples, and 20 fertilizer samples were collected from the Wuqing District of Tianjin City, China and was used to determine their HMs accumulation and potential ecological risks. Then, kriging interpolation and positive matrix factorization (PMF) were utilized to identify the sources of soil HMs. The results indicated that soil HMs in the study area were contaminated at a medium level, but that the pollution of Cd was more severe, and the Cd content in vegetables was slightly higher than the permissible threshold (0.02 mg·kg−1). Furthermore, a non-homogeneous distribution was observed, with higher concentrations of HM contaminants concentrated in the southwest of the study area, where many metal manufacturing industries are located. Our results suggest that the Cd originated from industrial activity; As and Pb from agricultural practices; Ni, Cu, Cr, and As mainly from natural sources; Zn and Cu from organic fertilizer; Pb and Cd mainly from traffic discharge; and Cr, Ni, and Pb from sewage irrigation. Obviously, the accumulation of soil HMs in the study area could be mainly attributed to industrial activities, implying the need for implementation of government strategies to reduce industrial point-source pollution.

The Effect of Vanadium Content Coupling with Heat Treatment Process on the Properties of Low-Vanadium Wear-Resistant Alloy

The development of wear-resistant materials with excellent properties is of great research value in the manufacturing industry. In this paper, a new kind of low-vanadium wear-resistant alloy was designed and characterized to unveil the influence of vanadium content coupling with heat treatment on the microstructure, hardness, and abrasive wear property. The performances of commercial high chromium cast iron (HCCI) and the new low-vanadium wear-resistant alloy are compared. The alloy with 3 wt.% vanadium quenched at 900 °C and tempered at 250 °C, possessing VC, Mo2C, and M7C3 distributed in the martensite matrix, displayed a wear resistance two times better than the HCCI. The results showed that the increase of vanadium content from 0.98 wt.% to 3.00 wt.% resulted in a decrease in the size of M7C3 and a more homogeneous distribution of M7C3. VC with a bimodal distribution is effective for impeding grooving or indenting by abrasives because of their high hardness, which plays a vital role in improving the wear resistance of low-vanadium wear-resistant alloy.

Mixed Magnetic Behavior in Gadolinium and Ruthenium Co-doped Nickel Oxide Nanoparticles

Pure and different concentrations from (Gd, Ru) co-doped NiO nanoparticles, capped with Polyvinylpyrrolidone (PVP), were fabricated by the co-precipitation method. The nanoparticles were characterized by different techniques. The Rietveld refinements of X-Ray Diffraction (XRD) patterns confirmed the formation of the pure face-centered-cubic NiO phase. The X-ray Photo-induced Spectroscopy (XPS) assured the trivalent oxidation state of the doped ions Gd3+ and Ru3+ and unveiled the multiple oxidation states of nickel ions (Ni2+ and Ni3+), emerging from the vacancies in the samples. The Transmission Electron Microscope (TEM) images showed the pseudospherical morphology of the samples and the Energy Dispersive X-ray permitted the quantitative analysis of the presented elements and their homogeneous distribution. The Raman and Fourier Transform Infra-Red (FTIR) spectra depicted the fundamental vibrational bands of NiO nanoparticles, confirming their purity. The UV-visible spectroscopy enabled the absorption measurements and the energy gap calculations. The co-dopants increased the energy bandgap of NiO nanoparticles from 3.15 eV for pure NiO to 3.62 eV with the highest concentration of the co-dopants (x = 0.02) The photoluminescence (PL) spectra gave insights into the possible defects present in the samples, such as nickel vacancies, single and double oxygen vacancies, and oxygen interstitials. The Vibrating Sample Magnetometer (VSM) studied the room temperature M-H loops of the co-doped samples. A combination of ferromagnetic, antiferromagnetic, and paramagnetic contributions was noticed and treated according to the law of approach to saturation and bound magnetic polaron (BMP) model. The magnetic parameters, such as the saturation magnetization, exchange and anisotropy field, and the BMP concentration were extracted from the fitted models and discussed in terms of the co-dopants’ concentration. The co-doped samples showed a softer magnetic behavior, which is recommended for data storage applications.

Cyclodextrins in Polymer-Based Active Food Packaging: A Fresh Look at Nontoxic, Biodegradable, and Sustainable Technology Trends

Using cyclodextrins (CDs) in packaging technologies helps volatile or bioactive molecules improve their solubility, to guarantee the homogeneous distribution of the complexed molecules, protecting them from volatilization, oxidation, and temperature fluctuations when they are associated with polymeric matrices. This technology is also suitable for the controlled release of active substances and allows the exploration of their association with biodegradable polymer targeting to reduce the negative environmental impacts of food packaging. Here, we present a fresh look at the current status of and future prospects regarding the different strategies used to associate cyclodextrins and their derivatives with polymeric matrices to fabricate sustainable and biodegradable active food packaging (AFP). Particular attention is paid to the materials and the fabrication technologies available to date. In addition, the use of cutting-edge strategies, including the trend of nanotechnologies in active food packaging, is emphasized. Furthermore, a critical view on the risks to human health and the associated updated legislation is provided. Some of the more representative patents and commercial products that currently use AFP are also listed. Finally, the current and future research challenges which must be addressed are discussed.

Effectiveness of a Nanohydroxyapatite-Based Hydrogel on Alveolar Bone Regeneration in Post-Extraction Sockets of Dogs with Naturally Occurring Periodontitis

Pathological mandibular fracture after dental extraction usually occurs in dogs with moderate to severe periodontitis. A nanohydroxyapatite-based hydrogel (HAP hydrogel) was developed to diminish the limitations of hydroxyapatite for post-extraction socket preservation (PSP). However, the effect of the HAP hydrogel in dogs has still not been widely investigated. Moreover, there are few studies on PSP in dogs suffering from clinical periodontitis. The purpose of this study was to evaluate the effectiveness of the HAP hydrogel for PSP in dogs with periodontitis. In five dogs with periodontitis, the first molar (309 and 409) of each hemimandible was extracted. Consequently, all the ten sockets were filled with HAP-hydrogel. Intraoral radiography was performed on the day of operation and 2, 4, 8 and 12 weeks post operation. The Kruskal–Wallis test and paired t-test were adopted for alveolar bone regeneration analysis. The results demonstrated that the radiographic grading, bone height measurement, and bone regeneration analysis were positively significant at all follow-up times compared to the day of operation. Moreover, the scanning electron microscopy with energy-dispersive X-ray spectroscopy imaging after immersion showed a homogeneous distribution of apatite formation on the hydrogel surface. Our investigation suggested that the HAP hydrogel effectively enhances socket regeneration in dogs with periodontitis and can be applied as a bone substitute for PSP in veterinary dentistry.