Chapter 30 Flow Cytometric Cell-Kinetic Analysis by Simultaneously Staining Nuclei with Propidium Iodide and Fluorescein Isothiocyanate

1990 ◽  
pp. 315-323 ◽  
Author(s):  
Alan Pollack
Keyword(s):  
Kinetic Analysis ◽  
Propidium Iodide ◽  
Fluorescein Isothiocyanate ◽  
Cell Kinetic ◽  
Flow Cytometric ◽  
Flow Cytometric Cell

scholarly journals Solid KHT tumor dispersal for flow cytometric cell kinetic analysis

Cytometry ◽  
1981 ◽  
Vol 2 (2) ◽  
pp. 54-58 ◽  
Author(s):  
M. G. Pallavicini ◽  
L. J. Folstad ◽  
C. Dunbar
Keyword(s):  
Kinetic Analysis ◽  
Cell Kinetic ◽  
Flow Cytometric ◽  
Flow Cytometric Cell

scholarly journals Flow cytometric evaluation of DNA stainability with propidium iodide after histone H1 extraction

Cytometry ◽  
1989 ◽  
Vol 10 (6) ◽  
pp. 726-730 ◽  
Author(s):  
Maria Chiara Giangarè ◽  
Ennio Prosperi ◽  
Guido Pedrali-Noy ◽  
Giovanni Bottiroli
Keyword(s):  
Propidium Iodide ◽  
Histone H1 ◽  
Flow Cytometric ◽  
Flow Cytometric Evaluation

Multi-parametric flow cytometric cell cycle analysis using TO-PRO-3 iodide (TP3): Detailed protocols

2008 ◽  
Vol 110 (3) ◽  
pp. 232-244 ◽  
Author(s):  
Michele Tavecchio ◽  
Matteo Simone ◽  
Sergio Bernasconi ◽  
Gianluca Tognon ◽  
Giuliano Mazzini ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Analysis ◽  
Flow Cytometric ◽  
Flow Cytometric Cell

Flow-cytometric cell counting and DNA estimation for the study of plant cell population dynamics

1994 ◽  
Vol 39 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Fernando T. Nicoloso ◽  
John Val ◽  
Maarten van der Keur ◽  
Frank van Iren ◽  
Jan W. Kijne
Keyword(s):  
Population Dynamics ◽  
Cell Population ◽  
Plant Cell ◽  
Cell Counting ◽  
Cell Population Dynamics ◽  
Flow Cytometric ◽  
Flow Cytometric Cell

Cell kinetic analysis of intact rat colonic crypts by confocal microscopy and immunofluorescence

1996 ◽  
Vol 111 (6) ◽  
pp. 1493-1500 ◽  
Author(s):  
H Konishi ◽  
G Steinbach ◽  
WN Hittelman ◽  
K Fujita ◽  
JJ Lee ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Kinetic Analysis ◽  
Cell Kinetic ◽  
Colonic Crypts

Cell kinetic analysis of human tumor xenografts using anti-bromodeoxyuridine monoclonal antibody

1987 ◽  
Vol 17 (1) ◽  
pp. 28-32 ◽  
Author(s):  
Shigehiro Kikuyama ◽  
Tetsuro Kubota ◽  
Masahiko Watanabe ◽  
Yoh Isobe ◽  
Takashi Fukutomi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Kinetic Analysis ◽  
Human Tumor ◽  
Cell Kinetic ◽  
Human Tumor Xenografts ◽  
Tumor Xenografts

Enrichment of tumor cells for cell kinetic analysis in human tumor biopsies using cytokeratin gating

1996 ◽  
Vol 41 (3) ◽  
pp. 237-248 ◽  
Author(s):  
K. Haustermans ◽  
I. Hofland ◽  
M. Ramaekers ◽  
D. Ivanyi ◽  
A.J.M. Balm ◽  
...  
Keyword(s):  
Kinetic Analysis ◽  
Tumor Cells ◽  
Human Tumor ◽  
Cell Kinetic ◽  
Tumor Biopsies

scholarly journals Chromosome doubling in Cattleya tigrina A. Rich

2019 ◽  
Vol 15 (11) ◽  
Author(s):  
Thays Saynara Alves Menezes-Sá ◽  
Maria de Fátima Arrigoni-Blank ◽  
Andréa Santos da Costa ◽  
Janay De Almeida Santos-Serejo ◽  
Arie Fitzgerald Blank ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Propidium Iodide ◽  
Chromosome Doubling ◽  
Flow Cytometric Analysis ◽  
Leaf Tissue ◽  
Stomatal Index ◽  
Chromosome Duplication ◽  
Flow Cytometric ◽  
Young Leaves ◽  
Commercial Value

Chromosome doubling induction in orchids may benefit their production for resulting in flowers of higher commercial value, larger size and higher content of substances that intensify the color and fragrance when compared with diploid orchids. This work aimed to induce and confirm artificial polyploidization, using flow cytometry and stomatal analysis. Explants were treated with colchicine at concentrations of 0, 2.5, 7.5, and 12.5 mM, for 24 and 48 hours and with oryzalin, at concentrations of 0, 10, 30, and 50 μM, for three and six days. For the flow cytometric analysis, a sample of leaf tissue was removed from each plant, crushed to release the nuclei and stained with propidium iodide. In addition to flow cytometry, the ploidy of the antimitotic treated plants was evaluated by stomata analysis. Young leaves were used where the density, functionality and stomatal index were evaluated. Colchicine provided induction of satisfactory polyploidy in C. tigrina at all concentrations and times of exposure, obtaining a greater number of polyploid individuals in the concentration of 12.5 mM for 48 hours. Oryzalin did not induce chromosome duplication at the tested concentrations.

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