Kinetic modeling of lactose hydrolysis with an immobilized β-galactosidase from Kluyveromyces fragilis

2000 ◽  
Vol 27 (8) ◽  
pp. 583-592 ◽  
Author(s):  
M Ladero ◽  
A Santos ◽  
F Garcı́a-Ochoa
1998 ◽  
Vol 22 (7) ◽  
pp. 558-567 ◽  
Author(s):  
A. Santos ◽  
M. Ladero ◽  
F. Garcı́a-Ochoa

2009 ◽  
Vol 5 (2) ◽  
Author(s):  
Alak Kumar Singh ◽  
Shishir Sinha ◽  
Karunakar Singh

Four yeast cultures, Kluyveromyces marxianus, Kluyveromyces fragilis, Saccharomyces marxianus and Saccharomyces fragilis, were selected for isolation of ?-galactosidase. Enzyme activity was measured for all four strains and found Kluyveromyces marxianus (177 ONPG unit/ml) to have the highest activity. Experimental whey was hydrolyzed with the enzyme extracted from K. marxianus, and the concentrations of enzyme used were 0.5, 1.0, 1.5, 2.0 and 2.5 ml of enzyme per litre of whey. The percentage of lactose hydrolysis was found to be a maximum of 80% in 60 minutes time for the enzyme concentration of 2.0 ml/ litre of whey.


1990 ◽  
Vol 87 ◽  
pp. 1159-1172 ◽  
Author(s):  
P Dagaut ◽  
M Cathonnet ◽  
B Aboussi ◽  
JC Boettner

2016 ◽  
Vol 136 (4) ◽  
pp. 254-262 ◽  
Author(s):  
Takahiro Yamazaki ◽  
Sho Sakaino ◽  
Toshiaki Tsuji

2017 ◽  
Author(s):  
Belinda Slakman ◽  
Richard West

<div> <div> <div> <p>This article reviews prior work studying reaction kinetics in solution, with the goal of using this information to improve detailed kinetic modeling in the solvent phase. Both experimental and computational methods for calculating reaction rates in liquids are reviewed. Previous studies, which used such methods to determine solvent effects, are then analyzed based on reaction family. Many of these studies correlate kinetic solvent effect with one or more solvent parameters or properties of reacting species, but it is not always possible, and investigations are usually done on too few reactions and solvents to truly generalize. From these studies, we present suggestions on how best to use data to generalize solvent effects for many different reaction types in a high throughput manner. </p> </div> </div> </div>


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