A differential scanning calorimetric study of the effects of metal ions, substrate/product, substrate analogues and chaotropic anions on the thermal denaturation of yeast enolase 1

2001 ◽  
Vol 28 (3) ◽  
pp. 213-218 ◽  
Author(s):  
John M. Brewer ◽  
John E. Wampler
Keyword(s):  
Metal Ions ◽  
Thermal Denaturation ◽  
Differential Scanning Calorimetric ◽  
Calorimetric Study ◽  
Substrate Analogues ◽  
Chaotropic Anions ◽  
Yeast Enolase

scholarly journals Differential-scanning-calorimetric study of the irreversible thermal denaturation of 8 kDa cytotoxin from the sea anemone Radianthus macrodactylus

1994 ◽  
Vol 299 (3) ◽  
pp. 731-733 ◽  
Author(s):  
G G Zhadan ◽  
V L Shnyrov
Keyword(s):  
Thermal Denaturation ◽  
Kinetic Constant ◽  
Sea Anemone ◽  
Differential Scanning Calorimetric ◽  
Calorimetric Study ◽  
Order Kinetic ◽  
Experimental Conditions ◽  
Rate Dependent ◽  
Scan Rate ◽  
State Kinetic

A differential-scanning-calorimetric study of the thermal denaturation of a sea-anemone (Radianthus macrodactylus) 8 kDa cytolytic toxin was carried out. The calorimetric traces were found to be irreversible and scan-rate-dependent under the experimental conditions employed. Scan-rate-dependent thermograms were explained in terms of a two-state kinetic model N k -->D, where k is a first-order kinetic constant that changes with temperature as given by the Arrhenius equation, N is the native state of the toxin, and D the denatured one.

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