Comparison of ethidium bromide-stained agarose gel electrophoresis and automated fragment analysis for evaluation of IgH gene products

2001 ◽  
Vol 25 (9) ◽  
pp. 769-774 ◽  
Author(s):  
Beate Gleissner ◽  
Juergen Maurer ◽  
Annette Sindram ◽  
Richard Reinhard ◽  
Eckhard Thiel
Keyword(s):  
Ethidium Bromide ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Gene Products ◽  
Agarose Gel Electrophoresis ◽  
Fragment Analysis

Comparative analysis of the DNA staining efficiencies of different fluorescent dyes in preparative agarose gel electrophoresis

2005 ◽  
Vol 43 (8) ◽  
Author(s):  
Qing Huang ◽  
Wei-Ling Fu
Keyword(s):  
Comparative Analysis ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ethidium Bromide ◽  
Gel Electrophoresis ◽  
Fluorescent Dyes ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Dna Staining

AbstractEthidium bromide (EB) is a mutagen and toxin that is widely used in the laboratory for visualization of nucleic acids. Safer nucleic acid stains, such as SYBR

Genotyping microsatellite polymorphisms by agarose gel electrophoresis with ethidium bromide staining

1998 ◽  
Vol 8 (4) ◽  
pp. 227-233 ◽  
Author(s):  
T. N. Ferraro ◽  
J. F. Schill ◽  
C. Ballas ◽  
N. Mulholland ◽  
G. T. Golden ◽  
...  
Keyword(s):  
Ethidium Bromide ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Ethidium Bromide Staining

scholarly journals PATTERNS OF MITOCHONDRIAL DNA VARIATION IN INDIGENOUS MAIZE RACES OF LATIN AMERICA

Genetics ◽  
1983 ◽  
Vol 104 (2) ◽  
pp. 365-379
Author(s):  
A K Weissinger ◽  
D H Timothy ◽  
C S Levings ◽  
M M Goodman
Keyword(s):  
Latin America ◽  
Gel Electrophoresis ◽  
South American ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Male Sterile ◽  
Fragment Analysis ◽  
Dna Variation ◽  
Mitochondrial Dnas ◽  
Ecori Digestion

ABSTRACT Mitochondrial DNAs (mtDNAs) were isolated from 93 diverse races of maize from Latin America. DNAs were examined by agarose gel electrophoresis of undigested DNA and by BamHI and EcoRI cleavage fragment analysis. Eighteen races contained plasmid-like mtDNAs. One race contained the S-1 and S-2 molecules associated with the S cytoplasmic male-sterile, and 17 were found to have the R-1 and R-2 plasmid-like DNAs. BamHI digestion of mtDNAs generated ten distinct electrophoretograms, and EcoRI digestion produced eight different fragment patterns. Races were assigned to one of 18 groups according to EcoRI and BamHI fragment patterns and whether or not they contained plasmid-like DNAs. Eight races produced restriction patterns similar to one of the characterized cytoplasmic male-steriles C, T, or S. Races from Meso-America and some from South America with Meso-American affinities were separated from other South American races. South American races were placed in three general classes of related groups. There was considerable agreement among the groupings here and those based on morphological and cytological affinities.

scholarly journals A comparison of DNA stains and staining methods for Agarose Gel Electrophoresis

2019 ◽  
Author(s):  
Andie C. Hall
Keyword(s):  
Nucleic Acid ◽  
Ethidium Bromide ◽  
Gel Electrophoresis ◽  
Careful Consideration ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Cost Increase ◽  
Dna Migration ◽  
Discernible Effect ◽  
Staining Methods

ABSTRACTNucleic acid stains are necessary for Agarose Gel Electrophoresis (AGE). The commonly used but mutagenic Ethidium Bromide is being usurped by a range of safer but more expensive alternatives. These safe stains vary in cost, sensitivity and the impedance of DNA as it migrates through the gel. Modified protocols developed to reduce cost increase this variability. In this study, five Gel stains (GelRed™, GelGreen™, SYBR™ safe, SafeView and EZ-Vision®In-Gel Solution) two premixed loading dyes (SafeWhite, EZ-Vision®One) and four methods (pre-loading at 100x, pre-loading at 10x, precasting and post-staining) are evaluated for sensitivity and effect on DNA migration. GelRed™ was found to be the most sensitive while the EZ-Vision® dyes and SafeWhite had no discernible effect on DNA migration. Homemade loading dyes were as effective as readymade ones at less than 4% of the price. This method used less than 1% of the dye needed for the manufacturer recommended protocols. Thus, with careful consideration of stain and method, Gel stain expenditure can be reduced by over 99%.

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

1988 ◽  
Vol 60 (02) ◽  
pp. 133-136 ◽  
Author(s):  
R Schneppenheim ◽  
H Plendl ◽  
U Budde
Keyword(s):  
Von Willebrand Factor ◽  
Gel Electrophoresis ◽  
Detection Methods ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Luminescence Assay ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

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