On the role of D-glucuronic acid in the aggregation of cells from the marine sponge geodia cydonium

Plasma and red blood cell (RBC) concentrations of normetanephrine (NMN), in both free and glucuronide-conjugated forms, were measured before, during, and after forced immobilization, an intense stressor of the sympathoadrenal system of rats. In this study NMN glucuronide was deconjugated by enzymatic hydrolysis; free and total NMN were assayed by radioenzymatic, thin-layer chromatographic procedures. In plasma, free NMN and NMN glucuronide are 777 +/- 99 and 792 +/- 74 pg/ml, respectively, when rats are at rest. Both free NMN and NMN glucuronide increased about 200% after 15 min of stress; in absolute amounts, increases were equivalent to that of the simultaneous increase in norepinephrine (NE). At 2 h of stress, NMN glucuronide, but not free NMN, increased further and significantly. The mean concentration of RBC-free NMN is about 50 times higher than that of plasma-free NMN, and it did not change significantly during stress; RBCs do not contain conjugated NMN. RBC NMN levels showed a strong correlation with RBC catechol methyltransferase activity. The latter seems to operate under conditions of substrate saturation; an acute release of NE leads to temporary storage of NE in RBCs but not conversion to NMN. The results indicate that conjugation of NMN with glucuronic acid is an important route for inactivation of plasma NMN formed during forced immobilization stress, whereas free NMN does not accumulate in RBCs during stress.

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Role of UDP-Glucuronic Acid Decarboxylase in Xylan Biosynthesis in Arabidopsis

Molecular Plant ◽

10.1016/j.molp.2016.04.013 ◽

2016 ◽

Vol 9 (8) ◽

pp. 1119-1131 ◽

Cited By ~ 31

Author(s):

Beiqing Kuang ◽

Xianhai Zhao ◽

Chun Zhou ◽

Wei Zeng ◽

Junli Ren ◽

...

Keyword(s):

Glucuronic Acid ◽

Acid Decarboxylase ◽

Xylan Biosynthesis

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THE ACID-SOLUBLE NUCLEOTIDES OF SALMON LIVER

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o58-052 ◽

1958 ◽

Vol 36 (5) ◽

pp. 465-473 ◽

Cited By ~ 12

Author(s):

H. Tsuyuki ◽

Violet M. Chang ◽

D. R. Idler

Keyword(s):

Low Temperature ◽

Anion Exchange ◽

Rat Liver ◽

Glucuronic Acid ◽

Anion Exchange Chromatography ◽

Exchange Chromatography ◽

Uridine Diphosphate ◽

Carbohydrate Storage ◽

Diphosphate Glucose

The acid-soluble nucleotides of spring salmon liver have been separated by anion-exchange chromatography at low temperature and characterized. Under these conditions the relatively labile uridine-5′-diphosphate nucleotides of acetylglucosamine, galactose, and glucuronic acid were obtained intact, a fact that is further substantiated by the complete absence of uridine-5′-diphosphate. The occurrence of these uridine diphosphate compounds and the absence of uridine diphosphate glucose is discussed in relation to the previously postulated role of inositol as a carbohydrate storage product. A new peptide-containing nucleotide, succinoadenosine-5′-phosphosulphate (peptide), was found in the fraction which immediately follows adenosine-5′-diphosphate. The parent base of this nucleotide, succinoadenine, was also isolated. The nucleotide pattern is simpler than that reported by other investigators for rat liver and wheat.

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THE METABOLISM OF INOSITOL IN SALMON: II. THE ROLE OF NUCLEOTIDES IN RELATION TO THE METABOLISM OF 2-C14-MYOINOSITOL IN COHO LIVER

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y60-147 ◽

1960 ◽

Vol 38 (1) ◽

pp. 1177-1183 ◽

Cited By ~ 1

Author(s):

H. Tsuyuki ◽

D. R. Idler

Keyword(s):

Uronic Acid ◽

Glucuronic Acid ◽

Acid Fraction ◽

Biochemical Reactions ◽

Entire Spectrum ◽

Sugar Nucleotide ◽

Measurable Activity

Though radioactivity is distributed throughout the entire spectrum of coho liver nucleotide fractions, the UDP-uronic acid fraction was found to be one of the more active. Most of the radioactivity was present in the glucuronic acid and glucuronolactone formed when UDP-uronic acid was hydrolyzed with acid. UDP-glucose, UDP-galactose, and UDP-N-acetylhexosamine did not contain any measurable activity. In C14-coho II another major concentration of activity was found in fraction A5, which has not yet been investigated. These results are discussed in relation to the known biochemical reactions of inositol and sugar nucleotide interconversions in plants.

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