The rate of aldehyde fixation of the exocytotic machinery in ultured hippocampal synapses

1997 ◽  
Vol 76 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Christian Rosenmund ◽  
Charles F. Stevens
Keyword(s):  
Aldehyde Fixation ◽  
Hippocampal Synapses

Cellular Contacts Within the Interhemal Membrane of the Rat Placenta at Term

1974 ◽  
Vol 32 ◽  
pp. 146-147
Author(s):  
William P. Jollie
Keyword(s):  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Capillary Endothelium ◽  
En Bloc ◽  
Aldehyde Fixation ◽  
Chorioallantoic Placenta

By routine EM preparative techniques, the tissues which, collectively, separate maternal and fetal bloods in the fully formed chorioallantoic placenta of the rat have been shown to consist of three chorionic layers, or trophoblast, and a layer of allantoic capillary endothelium [Fig. 1]. Relationships between these layers are best demonstrated by special techniques, viz., cacodylate-buffered aldehyde fixation, collidine-buffered osmium tetroxide postfixation, and en bloc staining with uranyl acetate. By using this method on placentas at term, the cells of the outermost chorionic layer (Trophoblast 1) appear to be attached to each other by means of maculae adherentes which sometimes occur in clusters [Fig. 2].

Complex Vesicles, Microtubules, and Cytoplasmic Filaments in the Endothelial Cells of Normal Dog Aorta

1968 ◽  
Vol 26 ◽  
pp. 172-173
Author(s):  
C. N. Sun ◽  
J. J. Ghidoni
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Cross Sections ◽  
Functional Significance ◽  
Tubular Structures ◽  
Aldehyde Fixation ◽  
Cytoplasmic Filaments

Endothelial cells in longitudinal and cross sections of aortas from 3 randomly selected “normal” mongrel dogs were studied by electron microscopy. Segments of aorta were distended with cold cacodylate buffered 5% glutaraldehyde for 10 minutes prior to being cut into small, well oriented tissue blocks. After an additional 1-1/2 hour period in glutaraldehyde, the tissue blocks were well rinsed in buffer and post-fixed in OsO4. After dehydration they were embedded in a mixture of Maraglas, D.E.R. 732, and DDSA.Aldehyde fixation preserves the filamentous and tubular structures (300 Å and less) for adequate demonstration and study. The functional significance of filaments and microtubules has been recently discussed by Buckley and Porter; the precise roles of these cytoplasmic components remains problematic. Endothelial cells in canine aortas contained an abundance of both types of structures.

scholarly journals Author Correction: Presynaptic endoplasmic reticulum regulates short-term plasticity in hippocampal synapses

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Nishant Singh ◽  
Thomas Bartol ◽  
Herbert Levine ◽  
Terrence Sejnowski ◽  
Suhita Nadkarni
Keyword(s):  
Endoplasmic Reticulum ◽  
Short Term ◽  
Short Term Plasticity ◽  
Hippocampal Synapses

scholarly journals Shrinkage of Dendritic Spines Associated with Long-Term Depression of Hippocampal Synapses

Neuron ◽  
2004 ◽  
Vol 44 (5) ◽  
pp. 749-757 ◽  
Author(s):  
Qiang Zhou ◽  
Koichi J. Homma ◽  
Mu-ming Poo
Keyword(s):  
Dendritic Spines ◽  
Long Term Depression ◽  
Hippocampal Synapses

Asynchronic transmission in the CA3–CA1 hippocampal synapses in the neurological mutanttaiep rat

2006 ◽  
Vol 85 (1) ◽  
pp. 223-229 ◽  
Author(s):  
Christian Bonansco ◽  
Marco Fuenzalida ◽  
Virginia Olivares ◽  
Carolina Molina ◽  
Manuel Roncagliolo
Keyword(s):  
Hippocampal Synapses
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