Role of salt bridge(s) in the binding and photoconversion of bilirubin bound to high affinity site on human serum albumin

2000 ◽  
Vol 1479 (1-2) ◽  
pp. 103-113 ◽  
Author(s):  
Mohd.Mushahid Khan ◽  
Salman Muzammil ◽  
Saad Tayyab
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Salt Bridge ◽  
High Affinity ◽  
High Affinity Site

scholarly journals Lysine residue 240 of human serum albumin is involved in high-affinity binding of bilirubin

1978 ◽  
Vol 171 (2) ◽  
pp. 453-459 ◽  
Author(s):  
C Jacobsen
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Lysine Residue ◽  
Water Soluble ◽  
High Affinity ◽  
Coupling Reagent ◽  
Peptide Fraction ◽  
Short Period ◽  
High Affinity Site

Bilirubin can be coupled covalently to albumin by using water-soluble carbodi-imide as coupling reagent. The optimal specificity in the attachment of bilirubin to the high-affinity site on the albumin molecule was obtained by treating an albumin-bilirubin complex with carbodi-imide in low concentrations and for a short period. The product was reduced, carboxymethylated and digested with trypsin. By fractionation on Sephadex G-50 (superfine grade) a peptide fraction containing most of the bilirubin label was isolated. Further purification by paper chromatography gave one peptide, consisting of residues 240-258. The peptide containined a single lysine residue, 240, and had an intact disulphide bridge. The results indicate that bilirubin is bound to lysine residue 240 at its high-affinity site on human serum albumin.

scholarly journals Role of cationic carbosilane dendrons and metallic core of functionalized gold nanoparticles in their interaction with human serum albumin

2018 ◽  
Vol 118 ◽  
pp. 1773-1780 ◽  
Author(s):  
Dzmitry Shcharbin ◽  
Elzbieta Pedziwiatr-Werbicka ◽  
Tatyana Serchenya ◽  
Sylwia Cyboran-Mikolajczyk ◽  
Lena Prakhira ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Metallic Core ◽  
Functionalized Gold Nanoparticles

scholarly journals Biological characteristics of two lysines on human serum albumin in the high-affinity binding of 4Z,15Z-bilirubin-IXα revealed by phage display

FEBS Journal ◽  
2011 ◽  
Vol 278 (21) ◽  
pp. 4100-4111 ◽  
Author(s):  
Ai Minomo ◽  
Yu Ishima ◽  
Ulrich Kragh-Hansen ◽  
Victor T. G. Chuang ◽  
Makiyo Uchida ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Phage Display ◽  
Human Serum ◽  
Biological Characteristics ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
High Affinity

Role of surface charge on the interaction between carbon nanodots and human serum albumin

2018 ◽  
Vol 204 ◽  
pp. 484-494 ◽  
Author(s):  
Meng-Ying Li ◽  
Chang-Qing Xiao ◽  
Zi-Qiang Xu ◽  
Miao-Miao Yin ◽  
Qi-Qi Yang ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Surface Charge ◽  
Carbon Nanodots

Binding of cationic Cm-E2O-Cm gemini surfactants with human serum albumin and the role of β-cyclodextrin

2020 ◽  
Vol 312 ◽  
pp. 113365 ◽  
Author(s):  
Mohd. Akram ◽  
Farah Ansari ◽  
Faizan Abul Qais ◽  
Kabir-ud-Din
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Gemini Surfactants

The role of human serum albumin and neurotoxin associated proteins in the formulation of BoNT/A products

Toxicon ◽  
2019 ◽  
Vol 168 ◽  
pp. 158-163 ◽  
Author(s):  
Anna Kutschenko ◽  
Hans Bigalke ◽  
Florian Wegner ◽  
Kai Wohlfarth
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Associated Proteins
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