Functional selection of phage displayed peptides for facilitated design of fusion tags improving aqueous two-phase partitioning of recombinant proteins

2002 ◽  
Vol 93 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Nina Bandmann ◽  
James Van Alstine ◽  
Andres Veide ◽  
Per-Åke Nygren
Keyword(s):  
Recombinant Proteins ◽  
Two Phase ◽  
Phase Partitioning ◽  
Selection Of

scholarly journals Preparative Purification of Recombinant Proteins: Current Status and Future Trends

2013 ◽  
Vol 2013 ◽  
pp. 1-18 ◽  
Author(s):  
Mayank Saraswat ◽  
Luca Musante ◽  
Alessandra Ravidá ◽  
Brian Shortt ◽  
Barry Byrne ◽  
...  
Keyword(s):  
High Performance ◽  
Cost Effective ◽  
Downstream Processing ◽  
Current Status ◽  
Comprehensive Overview ◽  
Two Phase ◽  
Phase Partitioning ◽  
Absolute Requirement ◽  
Flow Filtration ◽  
Selection Of

Advances in fermentation technologies have resulted in the production of increased yields of proteins of economic, biopharmaceutical, and medicinal importance. Consequently, there is an absolute requirement for the development of rapid, cost-effective methodologies which facilitate the purification of such products in the absence of contaminants, such as superfluous proteins and endotoxins. Here, we provide a comprehensive overview of a selection of key purification methodologies currently being applied in both academic and industrial settings and discuss how innovative and effective protocols such as aqueous two-phase partitioning, membrane chromatography, and high-performance tangential flow filtration may be applied independently of or in conjunction with more traditional protocols for downstream processing applications.

Apparatus for aqueous two-phase partitioning for terrestrial and space applications

1995 ◽  
Author(s):  
Mark Deuser ◽  
John Vellinger ◽  
Robert Naumann ◽  
Martin Guinn ◽  
Paul Todd
Keyword(s):  
Two Phase ◽  
Space Applications ◽  
Phase Partitioning

High absorption of endocrine disruptors by Hytrel: towards the development of a two-phase partitioning bioreactor

2012 ◽  
Vol 88 (1) ◽  
pp. 119-125 ◽  
Author(s):  
Julianne Ouellette ◽  
Silvia Cristina Cunha dos Santos ◽  
François Lépine ◽  
Pierre Juteau ◽  
Eric Déziel ◽  
...  
Keyword(s):  
Endocrine Disruptors ◽  
Two Phase ◽  
Phase Partitioning ◽  
Partitioning Bioreactor ◽  
High Absorption ◽  
Two Phase Partitioning Bioreactor
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