Radiation-induced polymerization monitored in situ by time-resolved fluorescence of probe molecules in methyl methacrylate

2003 ◽  
Vol 208 ◽  
pp. 405-410 ◽  
Author(s):  
Mark S. Frahn ◽  
Ruben D. Abellon ◽  
Leonard H. Luthjens ◽  
Martien J.W. Vermeulen ◽  
John M. Warman
Keyword(s):  
Methyl Methacrylate ◽  
Probe Molecules ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Radiation Induced

scholarly journals The use of peroxidase-mediated deposition of biotin-tyramide in combination with time-resolved fluorescence imaging of europium chelate label in immunohistochemistry and in situ hybridization.

1996 ◽  
Vol 44 (10) ◽  
pp. 1091-1099 ◽  
Author(s):  
R R de Haas ◽  
N P Verwoerd ◽  
M P van der Corput ◽  
R P van Gijlswijk ◽  
H Siitari ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Elongation Factor ◽  
Delayed Fluorescence ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Amplification System ◽  
Luciferase Mrna ◽  
Europium Chelate ◽  
Europium Chelates

The application of europium chelates as delayed fluorescent labels in FISH and immunocytochemistry is hampered by their relatively low quantum yield. To increase the intensity of the delayed fluorescence, we have used a recently introduced peroxidase-mediated amplification system. This system results in a large accumulation of biotin-tyramide, which is detected using streptavidin-europium chelate as label. Optimal staining conditions were evaluated for the immunocytochemical detection of vimentin in cryosections of rat liver, for DNA in situ hybridization (alphoid type probes and 40-KB cosmid probes), and for RNA in situ hybridization (detection of 28S ribosomal RNA, human elongation factor mRNA, and luciferase mRNA). Using a time-resolved fluorescence microscope, intense europium fluorescence was obtained in all these applications when the tyramide amplification system was applied. The signals were strong enough to be observed by eye using the microscope in the time-delayed mode. The routine application of this technique for localization and quantization of antigens or nucleic acid sequences in tissue exhibiting strong autofluorescence is discussed.

scholarly journals Time-resolved fluorescence imaging of europium chelate label in immunohistochemistry and in situ hybridization

Cytometry ◽  
1992 ◽  
Vol 13 (4) ◽  
pp. 329-338 ◽  
Author(s):  
Lahja Seveus ◽  
Mikko Väisälä ◽  
Stina Syrjänen ◽  
Minna Sandberg ◽  
Ari Kuusisto ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence Imaging ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Europium Chelate
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